EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:12876

Il33 interleukin 33 ( MGI:1924375)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011591. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011591_01	euxassay_011591_02	euxassay_011591_03	euxassay_011591_04

euxassay_011591_05	euxassay_011591_06	euxassay_011591_07	euxassay_011591_08	euxassay_011591_09

euxassay_011591_10	euxassay_011591_11	euxassay_011591_12	euxassay_011591_13	euxassay_011591_14

euxassay_011591_15	euxassay_011591_16	euxassay_011591_17	euxassay_011591_18	euxassay_011591_19

euxassay_011591_20	euxassay_011591_21	euxassay_011591_22	euxassay_011591_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12876_wholemount_strong.wlz

12876_wholemount_moderate.wlz

12876_wholemount_weak.wlz

12876_wholemount_possible.wlz

12876_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12876_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

adrenal cortex

strong

regional

strong expression: see section 10 11 12 13 19 20 21

endolymphatic duct

strong

regional

strong expression: see section 06 07 22 23 moderate expression: see section 02 03 04 05

utricle

strong

regional

strong expression: see section 06 22 moderate expression: see section 05

eye skeletal muscle

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 11 19 20 21 22 23

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 09 10 11 12 13 14 15 16 17 18 19 20

midgut

moderate

regional

moderate expression: see section 15 16 17 18 19 20 21 22

lower lip

moderate

regional

moderate expression: see section 13 14 15 16 17 18 19

urinary system mesentery

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 19 20 21 22 23

renal capsule

strong

regional

strong expression: see section 09 10 11 12 13 14 18 19 20 21 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T31653
Entity Detected:	Il33, interleukin 33 ( MGI:1924375)
Sequence:	sense strand is shown >T31653 TGTGCGTTCTGGGTTGAATGACTTAATGCTTCCAACTGAAGAAAGGGTAACAGAGAGAAAGAAAGCCATT CTTGGCTTACGATGTTGTGGAATGTTATTATGTAGAAAACTTCTTTTATTTCCTTTTCTTCAGCTACATG TTCAATAGACTCACAGATATATGACTTACGGCGTTGGTAAAGAAACTGAAGGAGATTCAGCCTTGCTCTT TCCTTTTCTCTGCCTTGAGTCCTGTATGAAATCACACTCACGGACTTCAGAAGAGCAGGCACCACAGTGC ATGGTTTGCTTTAGAGAGGGTCCATTTCAAAAACCTTCATAAAAACAATGCAAAACAAGAAAACAACCGA ACAAAAAAACCACCTATTTCCTGGTTCTAAACAAATGATTGTAATACTAGAGCAGTTAGTGGGAGGACCA GCTAGGGGGAGGATCACCTAGGGGAGGACCAGCTAGGGGGAGGACCAGCTGCTGCAAAGACTGACTGTTT CTCACTTATAATAAAATGCCAAATGCCTCCGCAGATGCCCCAGGCAACCCTCAGATCAGCCCTTTCTGTG AAGAGTGGCGTTACCTGTGCTTGTTTCCTTCTTAAACTTCCAATTTTTCTCTTTTAACACATTTAACATT TAACTTTAAGCAAGCCAGCTTACATTAGGAAGTGAAAGACATTTTAGTTCCCACCCGTGATTGAAATCAT TGACTATATCTAACAAGCTTAAAGTCTCCTGTAAGAACTGATCAGGATATACACTAGGACATGCCAATAG AATGGGATCTCATGGTGCAGTCTGAAGCCCTCC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:3593927), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 12039. Forward Primer - name:012039_F_IRAV08-11_K10_9230117N10Rik, sequence:TGTGCGTTCTGGGTTGAA; Reverse Primer - name:012039_R_SP6_IRAV08-11_K10_9230117N10Rik, sequence:TGGAGGGCTTCAGACTGC. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12872 same embryo
	EMAGE:12874 same embryo
	EMAGE:12873 same embryo
	EMAGE:12875 same embryo
	EMAGE:12871 same embryo
	EurExpress:euxassay_011591 same experiment
	MGI:4825580 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source