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EMAGE:12914

Thrsp thyroid hormone responsive SPOT14 homolog (Rattus) ( MGI:109126)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:12914 EMAGE:12914 EMAGE:12914 EMAGE:12914 EMAGE:12914
"Pseudo-wholemount" of euxassay_013671. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_013671_01 euxassay_013671_02 euxassay_013671_03 euxassay_013671_04
EMAGE:12914 EMAGE:12914 EMAGE:12914 EMAGE:12914 EMAGE:12914
euxassay_013671_05 euxassay_013671_06 euxassay_013671_07 euxassay_013671_08 euxassay_013671_09
EMAGE:12914 EMAGE:12914 EMAGE:12914 EMAGE:12914 EMAGE:12914
euxassay_013671_10 euxassay_013671_11 euxassay_013671_12 euxassay_013671_13 euxassay_013671_14
EMAGE:12914 EMAGE:12914 EMAGE:12914 EMAGE:12914 EMAGE:12914
euxassay_013671_15 euxassay_013671_16 euxassay_013671_17 euxassay_013671_18 euxassay_013671_19
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euxassay_013671_20 euxassay_013671_21 euxassay_013671_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:12914Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
12914_wholemount_strong.wlz
12914_wholemount_moderate.wlz
12914_wholemount_weak.wlz
12914_wholemount_possible.wlz
12914_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:12914_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T31657
Entity Detected:Thrsp, thyroid hormone responsive SPOT14 homolog (Rattus) ( MGI:109126)
Sequence:sense strand is shown

>T31657
CATGGATCGGTACTCCGCGGTGGTGCGGAACATGGAGCAGGTGGTGATGATCCCCAGCCTTCTGAGGGAT
GTGCAGCTGAGTGGGCCTGGGGGCTCGGTCCAGGACGGAGCCCCTGATCTCTATACCTACTTCACCATGC
TCAAGAGCATCTGTGTAGAAGTGGACCACGGGCTGCTGCCAAGGGAGGAATGGCAGGCCAAGGTGGCTGG
CAACGAAACCAGCGAGGCTGAGAACGACGCTGCTGAAACGGAGGAGGCCGAAGAAGACAGGATCTCGGAG
GAGCTGGACCTAGAAGCCCAGTTCCACCTGCACTTCTGCAGCCTCCATCACATCCTTACCCACCTGACCC
GGAAAGCACAGGAGGTGACGCGGAAATACCAGGAAATGACAGGGCAGGTCCTGTAGGTCTTTGACTCTTC
TGAAGATCGCTTTACACGAGAGAAGACTGATGAAGACCCCGAGGTGCATCAACCCGAGACCTGGACAGAG
GGCCTGGCCCCAGGTGGACGCGAAGGCGGCTTCCGTCTCCCTCTGAGTGCATCTGTGGACTTGGTTGCAG
GCTCACCTTTAAGATATTTGTGTGACTCCCAACTTCTCTTCCGTATCTTAGCAGTTTGAGGTTCACGAGG
CAGCAAGGATCAGTAGGAAGCACAGGTATTAACAAGTTGGCCAAAGTTCACGTCCCACCTCTGGGATGTC
GTTTAGTGCCTGGTGTCATGTCGCAGTTACTCTTTCATGTATAAAGCAGGGATGCTGTATCTACCTCACA
CGGTTACTAAAAGCAAACACGGAATCCAAAGCCCTGGGCACATGCCGACATCTGAAAAGCTGGTGTTTCT
CCCACCTCCCTCCGAGTGCATGCTGTAC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:2811774), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 12464. Forward Primer - name:012464_F_IRAV08-11_L18_Thrsp, sequence:CATGGATCGGTACTCCGC; Reverse Primer - name:012464_R_SP6_IRAV08-11_L18_Thrsp, sequence:CGTACAGCATGCACTCGG. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:12916 same embryo
 EMAGE:12913 same embryo
 EMAGE:12915 same embryo
 EurExpress:euxassay_013671 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS