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EMAGE:12938

Ubiad1 UbiA prenyltransferase domain containing 1 ( MGI:1918957)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:12938 EMAGE:12938 EMAGE:12938 EMAGE:12938 EMAGE:12938
"Pseudo-wholemount" of euxassay_013673. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_013673_01 euxassay_013673_02 euxassay_013673_03 euxassay_013673_04
EMAGE:12938 EMAGE:12938 EMAGE:12938 EMAGE:12938 EMAGE:12938
euxassay_013673_05 euxassay_013673_06 euxassay_013673_07 euxassay_013673_08 euxassay_013673_09
EMAGE:12938 EMAGE:12938 EMAGE:12938 EMAGE:12938 EMAGE:12938
euxassay_013673_10 euxassay_013673_11 euxassay_013673_12 euxassay_013673_13 euxassay_013673_14
EMAGE:12938 EMAGE:12938 EMAGE:12938 EMAGE:12938 EMAGE:12938
euxassay_013673_15 euxassay_013673_16 euxassay_013673_17 euxassay_013673_18 euxassay_013673_19
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euxassay_013673_20 euxassay_013673_21 euxassay_013673_22 euxassay_013673_23 euxassay_013673_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:12938Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
12938_wholemount_strong.wlz
12938_wholemount_moderate.wlz
12938_wholemount_weak.wlz
12938_wholemount_possible.wlz
12938_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:12938_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T31992
Entity Detected:Ubiad1, UbiA prenyltransferase domain containing 1 ( MGI:1918957)
Sequence:sense strand is shown

>T31992
CTTTGGCATCATCCTGGCACCAGCAGGCAGCCTGCCCAGACTCTGAGGAGACCAGCAACTCCCACCACAG
CACAGCCCCTCCTCAGGCAGTGCAGAAGCCAGAGACTGAGAAGGGGTGCCACTGGCCAGGGTGGGTGCAC
AGCATAGGCTGCAAACTCCTGAACTTGTGTGTTGGGATTATCTTCCTAAAGATAACCTGCTCGTGATTTG
TGTTCCATGTTACAGGGAAACCCTGAAGCCACTCTAGTGTCGTTCCTGTGTTATTTATAATCTCCACTAG
AGGGTGTCATCAGGCCACTTTAGGATGGAAAGTCCATCTCCCCCTTGTCCTAGACAGAATGCCCTGACAG
TTTGTGAAGGAGCTGTGTCCCAAAAGAGCCATAGTAACTAGGCATGGTACTGACCCGTTACCTTGGCACG
TAGGAAATTGTACTAATTGGTGCCAGGCCCCATTTATACAGAGCGCTTGTCCCCTGAATGTGCTGCCTGC
TAGAGAAGGGCCCTCATTAGCAAGGAGTGCTCCTGGCTTTTTGTGTAGTTGGTGGTTCAGATGTTTCTGT
CCTTTATGTCTCCCTTCGATCCTCGCCACTGTGGAGGAAAGTGTCAGATCACCCTGTAGCAAGACAGTTA
AGCCTGGGGCGGCTGAGAAAAGCACTCTGCTTTCAGTAAAACATGGCATGGTGGCTGAGGGAGCTGCTTC
CCTCTGTAGACTCCACTAGGAAGGCAGTGATTCTGGGACCTGAAGTGGCAGTGGGATGGGCGTTTAAAGC
CCAAAGGGCTTTGTCCCCTCCCAGCTGCCTCCCCTGGTAT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4241204), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 19477. Forward Primer - name:019477_F_IRAV28-31_N05_1200002M06Rik, sequence:CTTTGGCATCATCCTGGC; Reverse Primer - name:019477_R_SP6_IRAV28-31_N05_1200002M0, sequence:AATACCAGGGGAGGCAGC. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:12942 same embryo
 EMAGE:12940 same embryo
 EMAGE:12939 same embryo
 EMAGE:12941 same embryo
 EurExpress:euxassay_013673 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS