EMAGE mouse gene expression spatial database

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EMAGE:12966

Rrp8 ribosomal RNA processing 8, methyltransferase, homolog (yeast) ( MGI:1914251)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_013676. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_013676_01	euxassay_013676_02	euxassay_013676_03	euxassay_013676_04

euxassay_013676_05	euxassay_013676_06	euxassay_013676_07	euxassay_013676_08	euxassay_013676_09

euxassay_013676_10	euxassay_013676_11	euxassay_013676_12	euxassay_013676_13	euxassay_013676_14

euxassay_013676_15	euxassay_013676_16	euxassay_013676_17	euxassay_013676_18	euxassay_013676_19

euxassay_013676_20	euxassay_013676_21	euxassay_013676_22	euxassay_013676_23	euxassay_013676_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12966_wholemount_strong.wlz

12966_wholemount_moderate.wlz

12966_wholemount_weak.wlz

12966_wholemount_possible.wlz

12966_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12966_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

midbrain ventricular layer

moderate

regional

moderate expression: see section 08 10 12

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T2314
Entity Detected:	Rrp8, ribosomal RNA processing 8, methyltransferase, homolog (yeast) ( MGI:1914251)
Sequence:	sense strand is shown >T2314 TCGAGGNCAGATTCGGATCCTTGGGCTCCTCCGGCCCTGCGGGCTACGGGTGGTATGTTCGAAGAGCCCG AATGGGTGGAGGCGGCCCCAGCCATCGTGGGCCTAGGGGCCGCGACAGCACAGGTTAGGCCGGCGACTGC CCCGCCAGTCAAGGGACGCAAGCGCCGCCATCTCTTGGCCACATTACGGGCTCTGGAAGCAGCATCTCTC TCCCAACAAACCCCCAGCCTACCTGGCAGTGACTCTGAGGAGGAGGAGGAGGTAGGAAGGAAGAAGAGAC ACCTCCAAAGGCCCTCACTTGCCAGCGTCTCAAAGGAAGTAGGGAAGAAAAGAAAAGGGAAATGTCAGAA ACAGGCGCCATCCATCAGTGACTCTGAGGGGAAAGAAATAAGAAGAAAATGCCACAGACAAGCTCCTCCT CTTGGTGGGGTCTCTGCTGGAGAAGAAAAAGGAAAGAGAAAATGCCAGGAATATTCCTCTTTACACCTAA CCCAGCCCCTGGACAGTGTTGACCAAACAGTTCACAATTCCAGGACGAGTACTGCTACAATTGACCCATC CAA
Notes:	The probe template was PCR amplified from IMAGE:1139372 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1139372 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12967 same embryo
	EMAGE:12968 same embryo
	EMAGE:12969 same embryo
	EMAGE:12970 same embryo
	EurExpress:euxassay_013676 same experiment
	MGI:4827818 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source