Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:12985

Arhgef18 rho/rac guanine nucleotide exchange factor (GEF) 18 ( MGI:2142567)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:12985 EMAGE:12985 EMAGE:12985 EMAGE:12985 EMAGE:12985
"Pseudo-wholemount" of euxassay_013639. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_013639_01 euxassay_013639_02 euxassay_013639_03 euxassay_013639_04
EMAGE:12985 EMAGE:12985 EMAGE:12985 EMAGE:12985 EMAGE:12985
euxassay_013639_05 euxassay_013639_06 euxassay_013639_07 euxassay_013639_08 euxassay_013639_09
EMAGE:12985 EMAGE:12985 EMAGE:12985 EMAGE:12985 EMAGE:12985
euxassay_013639_10 euxassay_013639_11 euxassay_013639_12 euxassay_013639_13 euxassay_013639_14
EMAGE:12985 EMAGE:12985 EMAGE:12985 EMAGE:12985 EMAGE:12985
euxassay_013639_15 euxassay_013639_16 euxassay_013639_17 euxassay_013639_18 euxassay_013639_19
EMAGE:12985 EMAGE:12985 EMAGE:12985 EMAGE:12985 EMAGE:12985
euxassay_013639_20 euxassay_013639_21 euxassay_013639_22 euxassay_013639_23 euxassay_013639_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:12985Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
12985_wholemount_strong.wlz
12985_wholemount_moderate.wlz
12985_wholemount_weak.wlz
12985_wholemount_possible.wlz
12985_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:12985_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
metanephros
moderate moderate
regionalmoderate expression: see section 08 09 19 20 weak expression: see section 10 18
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T31646
Entity Detected:Arhgef18, rho/rac guanine nucleotide exchange factor (GEF) 18 ( MGI:2142567)
Sequence:sense strand is shown

>T31646
CCCCACCTCTGTGGAACAGGGAAGTGTCAGAGGCTGCTGGCATGAGCAAGAGCCCTCCAGAGCCAAGACC
TAAGCACTCATGAGCTCAGGGAGCGACTGTGGGCGTAGTTACCAAGAATCCAACCGCGAGCTAGTAAGAC
AACAGGGTGCTGAAAGTGGTGGTCTCTGACGACTTCCGGGTGCCAGGAATGGAGCAGGGTCCCCCTTGTC
CTCATCCCTAGGCCTATGGACCCCAGCACCCGAATAGCTCTTACTCTTGTCCTTCTGTTTCTATCTTGTG
TCCGGAGTGTGTCTATATCTTCCAGACCTCATTGTGCCATGTTGCTAATCAGTGTTTTGTGGTTTGTTTT
GTTGGTTTGGGTTTTGTTGTTGTTGTTGTTTTGTTTCTGTTTTTGTACTGAGATGTATCTGAGCACTTTA
GAAAAGCTGACTTTTAAGTTCCCACTCTGTGAAGAGAACATTCCAGTACCTAACAAGAGCTGGGTGGGCT
CACCACCAGACACCATGACTTTTTGGATTTAGAAGTTCAAATGGTTTCCACTTGGCCCTGGGAAGGATTC
AGTGTATCCCCCACCAGGTGGCCAGGTGGTTCTCAGCTGTGTACTGTAAACAGTAGGACAGTGTCATCAG
TTACCTGTCACCAGCAGCCATGGGTCCCTTCCTGGCAGATCTCATTTGTGAAGATGGTGAGCCAGCAGCA
TTCAATCTCCCTTGTCAATCGTGTCCATGATCTTCGCCTACCCCTGATTATTTTCACAGCGATAGCAGAG
CATCCTGGCAACAG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:2648223), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 13074. Forward Primer - name:013074_F_IRAV08-11_H16_Arhgef18, sequence:CCCCACCTCTGTGGAACA; Reverse Primer - name:013074_R_SP6_IRAV08-11_H16_Arhgef18, sequence:CCTGTTGCCAGGATGCTC. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:12986 same embryo
 EMAGE:12984 same embryo
 EMAGE:12988 same embryo
 EMAGE:12989 same embryo
 EMAGE:12987 same embryo
 EurExpress:euxassay_013639 same experiment
 MGI:4823231 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS