Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:12995

Pom121l2 POM121 membrane glycoprotein-like 2 (rat) ( MGI:2684870)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:12995 EMAGE:12995 EMAGE:12995 EMAGE:12995 EMAGE:12995
"Pseudo-wholemount" of euxassay_013647. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_013647_01 euxassay_013647_02 euxassay_013647_03 euxassay_013647_04
EMAGE:12995 EMAGE:12995 EMAGE:12995 EMAGE:12995 EMAGE:12995
euxassay_013647_05 euxassay_013647_06 euxassay_013647_07 euxassay_013647_08 euxassay_013647_09
EMAGE:12995 EMAGE:12995 EMAGE:12995 EMAGE:12995 EMAGE:12995
euxassay_013647_10 euxassay_013647_11 euxassay_013647_12 euxassay_013647_13 euxassay_013647_14
EMAGE:12995 EMAGE:12995 EMAGE:12995 EMAGE:12995 EMAGE:12995
euxassay_013647_15 euxassay_013647_16 euxassay_013647_17 euxassay_013647_18 euxassay_013647_19
EMAGE:12995 EMAGE:12995 EMAGE:12995 EMAGE:12995 EMAGE:12995
euxassay_013647_20 euxassay_013647_21 euxassay_013647_22 euxassay_013647_23 euxassay_013647_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:12995Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
12995_wholemount_strong.wlz
12995_wholemount_moderate.wlz
12995_wholemount_weak.wlz
12995_wholemount_possible.wlz
12995_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:12995_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T37789
Entity Detected:Pom121l2, POM121 membrane glycoprotein-like 2 (rat) ( MGI:2684870)
Sequence:sense strand is shown

>T37789
CTGGTGGGAAAGTTACCTGAAGCGTAATATTTGGTCCCTTCGTCACCCTCGGGCCACATGGAGTCCGGTG
ACAGTCAAGATCTCGCCTCCTGAGCGGAGACCACGGCCCTTGTTCCCGGTCTCAAGAGTGGTGAACTCCG
CGGGACCTTCAGAGGAGCCACCAGGGCCCTGTGGAAAGGACCCGGTGCTGAGGGCCCTCCTCCAGTGCAG
GAAGGGCAGAGCCAGGTGGGAAGAGCCACTGTTCCCGGACACCTCCGACAGCCAAAGGCATTCGGCCTCC
GTATGGTCCGCCTTTAAGCCCCTGTTGAAAAGTGGGGCCACTGTTTCGTTTGTGGCCAGGCCTGGGTCTC
TGAAAAGGAGACCCCATGTCCAGAGCTCAGATGATAAATGTAAGAGATCCGGTTGCTTCTCTGGGGCCCT
TGTGGCCAGCATACATACCCAAGGCCCCCCCAGCACAAAAAGAAATGCTATTACCAGTTCTTACAGTTCT
TCTAGAGAGCTCTCAGGATCTTGGAAGAGACATTTTCTCAGGACATTAGTCCAGACTCCAGAATGGCCAT
TGAAAAAAGCAGGAGAGAGTCCTAATTCTCACCTCTCAGTCACACCCAGCAGCTCTGGACAGCTAAATGG
AGAGATTCCTCTACTGCAGTCTGGCCCTAGGGACCTGCTAACTATACCTTGCCAGCAAGGTCCTGTTGTC
ACTGAAGAGGACCCAACATTAGAGGGGCAAGCTGTGCCGAGCAACCAAACCACAGAAGCTACAACCGGGA
CAGCCGGAGACTCCATTCCAGAGATGAGGGCTGGAATTCAGC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 84081. Forward Primer - name:084081_F_cDNA_LOC195236, sequence:CTGGTGGGAAAGTTACCTGAAG; Reverse Primer - name:084081_N_SP6_cDNA_LOC195236, sequence:GCTGAATTCCAGCCCTCATCT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:12991 same embryo
 EMAGE:12994 same embryo
 EMAGE:12993 same embryo
 EMAGE:12990 same embryo
 EMAGE:12992 same embryo
 EurExpress:euxassay_013647 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS