Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:13057

Alox5ap arachidonate 5-lipoxygenase activating protein ( MGI:107505)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:13057 EMAGE:13057 EMAGE:13057 EMAGE:13057 EMAGE:13057
"Pseudo-wholemount" of euxassay_017220. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_017220_01 euxassay_017220_02 euxassay_017220_03 euxassay_017220_04
EMAGE:13057 EMAGE:13057 EMAGE:13057 EMAGE:13057 EMAGE:13057
euxassay_017220_05 euxassay_017220_06 euxassay_017220_07 euxassay_017220_08 euxassay_017220_09
EMAGE:13057 EMAGE:13057 EMAGE:13057 EMAGE:13057 EMAGE:13057
euxassay_017220_10 euxassay_017220_11 euxassay_017220_12 euxassay_017220_13 euxassay_017220_14
EMAGE:13057 EMAGE:13057 EMAGE:13057 EMAGE:13057 EMAGE:13057
euxassay_017220_15 euxassay_017220_16 euxassay_017220_17 euxassay_017220_18 euxassay_017220_19
EMAGE:13057 EMAGE:13057 EMAGE:13057 EMAGE:13057
euxassay_017220_20 euxassay_017220_21 euxassay_017220_22 euxassay_017220_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:13057Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
13057_wholemount_strong.wlz
13057_wholemount_moderate.wlz
13057_wholemount_weak.wlz
13057_wholemount_possible.wlz
13057_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:13057_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T8253
Entity Detected:Alox5ap, arachidonate 5-lipoxygenase activating protein ( MGI:107505)
Sequence:sense strand is shown

>T8253
GGAAGGCTGAGGTGCTAGCACCAGCCTGGTTGTCTCTGGCGGGCCTGAAGCAAGCATGGATCAAGAGGCT
GTGGGCAACGTTGTGCTCCTGGCCCTTGTCACCCTCATCAGCGTGGTCCAGAATGCGTTCTTTGCCCACA
AGGTGGAGCATGAAAGCAAGGCGCATAATGGGAGAAGCTTCCAGAGGACCGGGACTCTTGCCTTTGAGCG
GGTCTACACTGCCAACCAGAACTGCGTAGATGCGTACCCCACTTTCCTTGTGGTACTCTGGACTGCAGGA
CTACTTTGCAGCCAAGTCCCTGCCGCCTTCGCCGGACTGATGTACCTGTTTGTGAGGCAAAAATACTTTG
TCGGCTATCTGGGAGAGAGAACTCAGAGCACCCCTGGCTACATCTTCGGCAAGCGGATCATCCTGTTTCT
GTTCCTCATGTCCTTCGCCGGGATACTCAACCATTACCTCATCTTCTTCTTCGGAAGCGACTTTGAGAAC
TACATCAGAACGGTAAGCACGACGATCTCCCCGCTGCTTCTCATCCCCTGATTGCTGGAGACAGAGAAGG
ACGCTCACCAGATCAATAGAGACGCATCATAACGCAATGCCGCGAAGGCTTCTGCTCCTCTTCAAGCTGT
AGATGCTGTCAATCTTGCTGCCCTCGGGGCTCTGTGGCATCCGTTAACTTTGCTTTTCCGGGAAGAAAAA
TGTCTTGTGCTAGCTCCACCCCTCGAATGCGGCGGTGGCCCAGGATTTATTGTCTACATCCAGCCTATAC
TTCTCCTGGCTTATCCTGCTTTCTGAAGATGTCTTGTAATCAGACACGTGTTTTCCTAAAATAAAGGGTA
TAGACAAAATTTAAAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:1349819 using vector (pT7T3D-PacI) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:1349819 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:13056 same embryo
 EMAGE:13059 same embryo
 EMAGE:13058 same embryo
 EMAGE:13061 same embryo
 EMAGE:13060 same embryo
 EurExpress:euxassay_017220 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS