EMAGE mouse gene expression spatial database

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EMAGE:13129

Lamb2 laminin, beta 2 ( MGI:99916)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_017324. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_017324_01	euxassay_017324_02	euxassay_017324_03	euxassay_017324_04

euxassay_017324_05	euxassay_017324_06	euxassay_017324_07	euxassay_017324_08	euxassay_017324_09

euxassay_017324_10	euxassay_017324_11	euxassay_017324_12	euxassay_017324_13	euxassay_017324_14

euxassay_017324_15	euxassay_017324_16	euxassay_017324_17	euxassay_017324_18	euxassay_017324_19

euxassay_017324_20	euxassay_017324_21	euxassay_017324_22	euxassay_017324_23	euxassay_017324_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13129_wholemount_strong.wlz

13129_wholemount_moderate.wlz

13129_wholemount_weak.wlz

13129_wholemount_possible.wlz

13129_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13129_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7185
Entity Detected:	Lamb2, laminin, beta 2 ( MGI:99916)
Sequence:	sense strand is shown >T7185 CCACGCGTCCGGGACACCAGCCCAGTACCCACACGGTCGGGATGGAGTGGGCCTCAGGAGAACCAGGGAG GGGCAGGCAGGGACAGCCTTTGCCATGGGAACTTCGCTTGGGCCTACTTCTAAGTGTGCTGGCTGCCACA TTGGCCCAGGCCCCGTCCTTGGATGTACCTGGCTGTTCTCGAGGAAGCTGCTATCCAGCCACCGGTGACC TGTTGGTGGGCCGTGCGGACAGACTGACGGCCTCATCCACGTGTGGCTTGCATAGCCCTCAACCCTACTG TATTGTCAGTCACCTGCAGGACGAAAAGAAGTGTTTCCTGTGTGACTCCCGACGTCCCTTCTCTGCTCGA GACAACCCAAATAGTCATCGGATCCAGAATGTAGTCACCAGCTTTGCGCCACAACGCCGGACGGCCTGGT GGCAATCGGAGAACGGGGTTCCAATGGTCACCATCCAACTGGACCTGGAAGCTGAGTTTCATTTCACCCA CCTCATTATGACGTTCAAGACGTTCCGGCCTGCTGCTATGCTGGTGGAGCGTTCTGCAGACTTTGGCCGC ACCTGGCACGTGTACCGATATTTTTCCTATGACTGCGGGGCTGACTTCCCGGGAATCCCACTGGCCCCGC CACGTCGCTGGGATGATGTAGTGTGTGAGTCCCGCTACTCAGAAATCGAGCCGTCTACGGAAGGCGAGGT CATCTATCGTGTGCTGGACCCTGCTATTCCTATCCCAGACCCCTACAGCTCACGGATTCAGAACCTGTTG AAGATCACCAACCTACGAGTGAACTTAACCCGGCTTCACACACTGGGAGACAACTTGCTTGACCCACGGA GGGAGATCCGGGAAAAATACTATTATGCTCTCTATGAACTTGTCATCCGTGGCAACTGCTTCTGCTATGG CCACGCCTCACAGTGTGCGCCTGCACCAGGGGCGCCGGCCCATGCTGAGGGCATGGTACACGGAGCCTGT ATCTGCAAGCACAATACTCGTGGACTCAACTGTGAGCAGTGTCAGGATTTCTATCAGGACCTTCCCTGGC ACCCTGCAGAGGACGGCCATACTCACGCCTGTCGGAAGTGTGAGTGCAACGGGCATACTCATAGCTGCCA CTTTGACATGGCTGTCTACCTGGCATCTGGAAATGTAAGTGGAGGCGTATGCGATGGGTGTCAGCACAAC ACAGCTGGGCGCCATTGTGAGTTCTGCCGGCCCTTCTTCTACCGTGACCCCACCAAGGACATGCGGGACC CAGCTGTGTGCCGTCCTTGTGACTGTGACCCTATGGGTTCTCAAGATGGTGGTCGCTGTGATTCTCATGA TGACCCTGTGCTAGGACTGGTCTCAGGCCAGTGTCGCTGCAAAGAACACGTGGTTGGCACTCGCTGCCAG CAATGCCGTGATGGCTTCTTTGGACTTAGTGCCAGTGACCCTCGAGGGTGCCAGCGTTGCCAGTGTAATT CACGGGGCACAGTGCCTGGGAGCTCCCCTTGTGACTCCAGTAGTGGAACCTGTTTCTGCAAGCGTCTGGT GACCGGACATGGCTGTGACCGCTGTCTGCCTGGCCACTGGGGCCTGAGCCATGACCTGCTGGGCTGCCGT CCCTGTGACTGTGATGTGGGCGGTGCCTTGGATCCTCAGTGTGATGAGGCCACCGGTCAGTGCCGCTGCC GCCAACACATGATTGGGCGGCGCTGCGAACAAGTGCAGCCTGGCTACTTCCGGCCTTTTCTGGACCATTT AACCTGGGAGGCTGAGGCTGCCCAAGGGCAGGGGCTTGAGGTGGTAGAGCGGCTGGTGACCAACCGAGAG ACTCCGTCCTGGACTGGCCCAGGCTTTGTGCGGCTGCGAGAAGGTCAGGAAGTGGAGTTCCTGGTGACCT CTTTGCCTAGGGCCATGGACTATGACCTGCTACTGCGCTGGGAGCCCCAGGTCCCTGAGCAATGGGCAGA GCTGGAACTGATGGTGCAGCGTCCGGGGCCTGTGTCTGCTCACAGTCCGTGCGGGCATGTGCTGCCTAAG GATGACCGCATTCAGGGGATGCTTCACCCAAACACCAGGTTTTTGGTGTTTCCCAGACCTGTCTGCCTTG AGCCTGGCATCTCCTACAAGCTGAAGCTGAAACTGATCGGAACAGGGGGACGAGCCCAGCCTGAAACCTC CTACTCTGGATTACTCATTGACTCGCTGGTCCTGCAGCCCCACGTCTTGGTGCTAGAGATGTTTAGTGGG GGCGATGCTGCTGCTCTGGAGCGCCGTACCACCTTTGAACGCTACCGCTGCCATGAGGAAGGTCTGATGC CCAGCAAGGCCCCTCTATCTGAGACCTGTGCCCCCCTCCTCATCAGCGTGTCCGCCTTGATCTACAATGG CGCCTTGCCATGTCAGTGTGACCCTCAAGGCTCACTGAGTTCTGAATGCAGTCCTCACGGTGGCCAGTGC CGGTGCAAACCTGGAGTGGTTGGACGCCGTTGTGATGTCTGTGCTACTGGCTACTATGGCTTTGGCCCTG CAGGCTGTCAAGCCTGCCAGTGTAGTCCTGATGGAGCACTCAGTGCCCTCTGTGAAGGGACTAGTGGACA GTGCCCCTGCCGACCTGGTGCCTTTGGTCTTCGCTGTGACCACTGTCAACGTGGCCAGTGGGGATTCCCT AATTGCCGGCCGTGTGTCTGCAATGGGCGTGCGGATGAGTGTGATACCCACACAGGCGCTTGCCTGGGCT GCCGTGATTACACGGGGGGCGAGCACTGTGAAAGGTGCATTGCTGGTTTTCATGGGGACCCACGGCTGCC ATATGGGGGCCAGTGCCGGCCTTGTCCCTGCCCTGAAGGCCCTGGGAGCCAGCGACACTTTGCTACTTCT TGCCACCGGGATGGATATTCCCAGCAAATTGTGTGCCAGTGTCGAGAAGGCTACACAGGGCTTCGGTGTG AAGCTTGTGCCCCCGGGCACTTTGGGGACCCATCAAAGCCAGGTGGCAGGTGCCAACTGTGTGAGTGCAG TGGAAACATTGATCCCATGGACCCTGATGCCTGTGATCCCCACACGGGGCAATGCTTGCGTTGTTTACAC AACACAGAGGGGCCCCACTGTGGCTATTGCAAGCCTGGCTTCCATGGGCAAGCTGCCCGACAGAGCTGTC ACCGCTGTACCTGCAACCTTCTGGGCACAGATCCCAGGCGGTGCCCATCTACCGACCTGTGCCATTGTGA CCCAAGCACTGGGCAGTGCCCATGCCTTCCCCATGTCCAAGGCCTCAACTGTGACCATTGTGCCCCCAAC TTTTGGAACTTCACCAGTGGCCGTGGCTGCCAGCCTTGTGCTTGTCACCCAAGCCGGGCCAGAGGCCCTA CCTGCAATGAGTTCACAGGGCAGTGTCACTGTCATGCTGGCTTTGGTGGGAGGACTTGTTCTGAGTGCCA AGAGCTCTACTGGGGAGACCCTGGTCTGCAGTGCCGTGCCTGTGACTGTGATCCTAGAGGAATAGACAAA CCTCAGTGTCATCGTTCCACAGGCCACTGTAGCTGCCGCCCAGGCGTGTCTGGTGTGCGCTGTGACCAGT GTGCTCGTGGCTTCTCAGGGGTTTTTCCTGCTTGTCACCCCTGCCACGCTTGCTTTGGAGACTGGGATCG TGTGGTACAGGACCTGGCTGCTCGGACGCGGCGCCTGGAGCAGTGGGCTCAGGAGTTGCAGCAAACAGGA GTGCTGGGTGCCTTTGAGAGCAGCTTTTTGAACATGCAGGGGAAGCTAGGCATGGTGCAGGCCATTATGA GTGCCCGCAATGCCTCAGCCGCCTCTACGGCGAAGCTTGTAGAGGCCACAGAGGGACTACGTCATGAAAT CGGGAAGACCACCGAGCGCCTGACTCAGTTAGAAGCAGAGCTAACAGCTGTGCAGGATGAGAACTTCAAT GCCAACCATGCACTCAGTGGTCTGGAGAGAGACGGGCTTGCGCTTAATCTCACCCTGAGGCAGCTGGATC AGCATCTGGAGATCCTCAAACATTCAAATTTCTTAGGTGCCTATGACAGCATCCGACATGCCCACAGCCA GTCCACAGAGGCAGAGCGCCGTGCCAACGCCTCCACCTTTGCAGTACCCAGCCCTGTGAGCAACTCAGCA GATACCCGGCGTCGGACGGAAGTGCTAATGGGTGCCCAAAAAGAAAACTTCAACCGCCAACATTTGGCCA ACCAGCAGGCACTGGGACGGCTCTCTGCACATGCCCACACCCTGAGCCTGACGGGCATAAATGAGTTGGT GTGTGGGGCACCAGGGGACGCACCCTGTGCCACCAGCCCTTGTGGGGGTGCCGGATGTCGGGATGAAGAT GGGCAGCCCCGTTGTGGTGGCCTCGGTTGCAGTGGGGCAGCAGCCACGGCAGATCTAGCGCTGGGCCGGG CTCGGCACACGCAGGCAGAGCTGCAGCGGGCACTGGTAGAAGGTGGCGGCATCCTCAGCCGGGTGTCTGA GACTCGTCGGCAGGCAGAAGAGGCACAGCAGCGAGCACAGGCAGCCCTGGACAAGGCTAATGCTTCCAGG GGCCAGGTGGAACAGGCCAATCAGGAGCTTCGAGAACTTATCCAGAATGTGAAAGACTTCCTCAGCCAGG AGGGAGCCGATCCTGACAGTATTGAAATGGTAGCGACTCGGGTGCTAGACATCTCCATCCCGGCCTCACC CGAGCAGATCCAGCGCCTAGCCAGTGAGATTGCAGAACGCGTCCGAAGCCTGGCCGACGTGGACACAATC CTGGCCCATACCATGGGCGACGTGCGTCGGGCTGAACAGCTACTGCAAGATGCGCACCGGGCACGGAGCC GGGCCGAGGGTGAGAGACAGAAGGCAGAGACAGTCCAAGCGGCACTGGAGGAGGCTCAGAGGGCACAAGG AGCTGCTCAGGGTGCCATCTGGGGAGCAGTGGTTGACACACAAAACACAGAGCAGACCCTGCAGCGGGTC CAGGAGAGGATGGCAGGTGCAGAGAAGTCTCTGAACTCTGCCGGTGAGCGGGCTCGGCAATTAGACGCCC TCCTGGAGGCCCTGAAACTGAAACGGGCAGGAAATAGCCTGGCAGCATCTACAGCGGAAGAAACAGCAGG CAGTGCCCAGAGCCGTGCCAGGGAGGCTGAGAAACAACTACGGGAACAAGTAGGTGACCAATACCAAACA GTGAGGGCGTTGGCAGAGCGGAAGGCTGAAGGTGTTCTGGCTGCACAAGCCAGGGCAGAACAACTGCGGG ATGAGGCTCGGGACCTGTTGCAGGCCGCTCAGGATAAGCTGCAGCGGCTACAGGAGCTGGAGGGCACATA TGAGGAGAACGAGCGTGCACTGGAGGGCAAAGCGGCCCAGCTGGATGGGCTGGAAGCCAGGATGCGCAGT GTGCTCCAGGCCATCAACTTGCAGGTCCAGATCTACAACACCTGCCAGTGACCACTCCCTAGGGCCTAGC CTTGTCGCCAAGCACTGTTCTGCACACGATCGTCCGCACATTAAAGAGCTCCTGGCTAGCAAGAGCTTTC AATAAACCTGTGTGAACCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4036357 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4036357 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	BL6/SV129
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13134 same embryo
	EMAGE:13133 same embryo
	EMAGE:13132 same embryo
	EMAGE:13131 same embryo
	EMAGE:13130 same embryo
	EurExpress:euxassay_017324 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source