EMAGE mouse gene expression spatial database

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EMAGE:13151

Ltbp1 latent transforming growth factor beta binding protein 1 ( MGI:109151)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_017295. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_017295_01	euxassay_017295_02	euxassay_017295_03	euxassay_017295_04

euxassay_017295_05	euxassay_017295_06	euxassay_017295_07	euxassay_017295_08	euxassay_017295_09

euxassay_017295_10	euxassay_017295_11	euxassay_017295_12	euxassay_017295_13	euxassay_017295_14

euxassay_017295_15	euxassay_017295_16	euxassay_017295_17	euxassay_017295_18	euxassay_017295_19

euxassay_017295_20	euxassay_017295_21	euxassay_017295_22	euxassay_017295_23	euxassay_017295_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13151_wholemount_strong.wlz

13151_wholemount_moderate.wlz

13151_wholemount_weak.wlz

13151_wholemount_possible.wlz

13151_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13151_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9449
Entity Detected:	Ltbp1, latent transforming growth factor beta binding protein 1 ( MGI:109151)
Sequence:	sense strand is shown >T9449 GTTACCATTCTGATGTTGGAGCGGCCGCCGAACTGAATTTTTTTTTTTTTTTTTTCTTTTTCCTTTTCCT TTTTTTTTTTTTTTTTTTTTTTTCGGTTGAAAATTTTTCCAAGGCAAGTTCATGGATACTAAGCTGATGT GTTTGTTGTTCTTTCTCTGTCTGCCTCTACTCCTAGTGAGTAACCACACTGGTCGCATCAAGGTGGTCTT TACTCCGAGCATCTGTAAAGTGACCTGCACCAAGGGCAACTGTCAGAACAGCTGCCAGAAGGGGAATACC ACCACTCTCATTAGTGAGAACGGCCATGCAGCCGACACCCTGACAGCCACGAACTTCCGAGTGGTTATTT GCCATCTTCCGTGTATGAATGGTGGCCAGTGCAGTTCAAGGGACAAATGTCAGTGCCCTCCAAATTTCAC AGGAAAGCTTTGCCAGATCCCTGTCCTTGGTGCCAGTATGCCCAAACTCTACCAGCACGCCCAGCAGCAG GGCAAGGCATTGGGGAGTCACGTCATCCATTCCACACATACCTTGCCTCTAACCATGACTAGCCAGCAAG GGGTCAAAGTGAAATTCCCCCCCAACATAGTCAATATCCATGTGAAACATCCTCCCGAGGCTTCTGTCCA GATACACCAGGTTTCCAGGATTGACAGCCCAGGAGGCCAGAAGGTGAAGGAAGCCCAACCAGGCCAGTCC CAAGTCTCTTACCAAGGACTTCCGGTCCAGAAGACCCAGACAGTCCATTCTACGTACTCCCACCAGCAGC TTATTCCCCATGTGTATCCCGTGGCTGCTAAGACACAACTTGGCCGATGCTTCCAGGAAACCATTGGATC ACAGTGTGGCAAAGCGCTCCCTGGCCTTTCCAAGCAAGAGGACTGCTGTGGGACAGTGGGGACGTCCTGG GGCTTTAACAAATGCCAGAAGTGCCCCAAGAAACAATCCTATCATGGATATACTCAAATGATGGAATGCC TACAAGGATATAAACGGGTTAACAACACCTTTTGTCAAGATATTAATGAATGTCAGCTACAAGGTGTATG CCCTAATGGTGAGTGTTTGAATACCATGGGCAGCTACAGATGCTCCTGCAAAATGGGATTCGGGCCTGAC CCTACCTTTTCAAGTTGTGTTCCGGATCCCCCTGTGATCTCAGAAGAGAAAGGGCCCTGTTACCGCCTTG TCAGTCCTGGAAGGCATTGTATGCACCCTCTCTCTGTTCATCTCACCAAGCAGATCTGCTGTTGTAGTGT GGGCAAGGCCTGGGGCCCACATTGTGAGAAATGTCCCCTCCCAGGCACAGCTGCTTTTAAGGAAATCTGT CCTGGTGGAATGGGTTATACGGTTTCTGGCGTTCATAGACGCAGGCCAATCCATCAGCATATAGGTAAAG AAGCTGTATATGTCAAGCCAAAGAACACTCAACCTGTTGCTAAAAGTACTCATCCTCCACCTCTCCCAGC CAAGGAAGAGCCTGTGGAGGCCCTGACCTCCTCGTGGGAACACGGGCCGAGAGGGGCGGAGCCCGAAGTG GTAACAGCACCTCCTGAGAAGGAAATACCTTCACTGGATCAAGAGAAAACCAGACTTGAACCTGGGCAAC CCCAGCTCTCCCCAGGGGTTTCAACTATTCATCTGCACCCACAGTTTCCAGTAGTGGTTGAAAAGACATC CCCTCCTGTGCCTGTGGAAGTAGCTCCTGAAGCTTCCACATCCAGTGCCAGCCAAGTGATTGCTCCTACG CAAGTAACAGAAATCAACGAATGTACTGTGAACCCGGACATTTGCGGAGCAGGACACTGCATCAACCTGC CGGTGCGGTATACCTGCATATGCTACGAGGGCTACAAGTTCAGTGAACAGCTGAGGAAATGTGTCGACAT TGATGAATGTGCTCAGGTCCGGCACCTCTGCTCCCAGGGTCGCTGTGAGAACACAGAGGGAAGTTTCCTG TGTGTCTGCCCGGCAGGGTTTATGGCCAGTGAGGAGGGTACTAACTGTATAGATGTGGATGAGTGCCTGA GGCCTGATATGTGTAGGGATGGCCGCTGCATCAACACCGCTGGGGCCTTCCGGTGCGAGTACTGTGACAG TGGGTACCGGATGTCACGGAGAGGCTACTGTGAGGATATTGATGAGTGTCTGAAGCCAAGCACTTGTCCC GAGGAACAGTGTGTGAACACCCCGGGCTCTTACCAGTGTGTGCCCTGCACAGAGGGGTTCCGCGGCTGGA ATGGACAATGCCTTGATGTGGACGAGTGCCTGCAGCCAAAGGTCTGTACGAATGGTTCCTGCACCAACCT GGAAGGGTCCTACATGTGTTCCTGCCACAGGGGCTACAGCCCCACACCAGACCATAGACACTGTCAAGAT ATTGATGAATGCCAACAAGGGAATCTCTGCATGAATGGGCAGTGCAGAAATACCGATGGCTCCTTCCGGT GTACCTGTGGACAGGGCTATCAGCTGTCAGCAGCTAAAGACCAATGTGAAGATATTGATGAATGCGAGCA CCATCACCTCTGCTCTCACGGGCAGTGCAGGAACACAGAGGGCTCCTTCCAGTGTGTGTGCAACCAGGGT TACAGAGCATCTGTACTTGGAGACCACTGTGAGGATATCAATGAATGCTTGGAGGACAGCAGTGTCTGCC AGGGAGGTGACTGCATCAATACTGCGGGGTCCTATGATTGCACATGCCCGGATGGATTCCAGCTGAACGA CAATAAGGGCTGTCAAGACATTAATGAATGTGCACAGCCAGGACTCTGTGGATCTCATGGGGAGTGTCTA AACACACAAGGCTCCTTCCACTGTGTCTGTGAACAGGGGTTCTCCATCTCTGCCGATGGCCGTACATGCG AAGATATTGATGAGTGTGTTAACAACACTGTGTGTGACAGTCACGGCTTCTGTGACAACACAGCCGGCTC TTTCCGCTGCCTCTGTTATCAGGGCTTTCAAGCCCCACAGGATGGGCAAGGGTGTGTGGATGTGAACGAG TGTGAACTGCTCAGTGGTGTATGTGGGGAAGCCTTCTGTGAAAACGTGGAAGGATCCTTCCTGTGTGTGT GTGCCGATGAGAACCAGGAGTACAGCCCCATGACTGGGCAGTGTCGCTCTCGGGTCACGGAAGATTCAGG TGTGGATCGTCAGCCTAGAGAAGAGAAGAAGGAGTGCTATTATAACCTCAATGATGCTAGTCTCTGTGAT AATGTCCTGGCCCCCAACGTCACCAAACAAGAGTGCTGCTGTACGTCAGGTGCCGGCTGGGGAGACAACT GTGAGATCTTCCCTTGCCCCGTCCAGGGAACTGCTGAGTTCACGGAAATGTGCCCTAGAGGGAAAGGTTT GGTCCCGGCTGGAGAATCCTCTTACGACACTGGTGGCGAGAACTACAAAGATGCTGATGAATGCTTGCTG TTTGGAGAGGAAATCTGTAAAAATGGTTACTGTTTGAACACTCAACCTGGGTATGAATGCTACTGTAAGC AAGGGACATACTATGATCCTGTCAAATTACAGTGCTTTGATATGGATGAATGCCAAGACCCTAACAGTTG CATTGACGGCCAGTGTGTGAACACAGAGGGCTCCTACAACTGCTTTTGCACCCACCCAATGGTCCTGGAT GCCTCTGAGAAGAGATGTGTGCAACCAACTGAATCAAATGAACAAATAGAAGAGACCGATGTCTATCAAG ATCTGTGCTGGGAGCATCTGAGTGAGGAGTACGTGTGTAGCCGTCCTCTCGTAGGCAAGCAGACGACGTA CACAGAATGCTGCTGTTTGTACGGGGAGGCTTGGGGCATGCAGTGTGCTCTGTGCCCCATGAAGGACTCA GATGACTATGCCCAGCTGTGCAATATCCCTGTGACTGGGCGTCGGCGGCCATATGGACGGGATGCATTGG TGGACTTCAGTGAACAGTATGGCCCAGAAACAGACCCTTACTTCATCCAGGATCGCTTTCTAAATAGCTT TGAGGAGCTACAGGCTGAGGAGTGTGGCATCCTCAATGGTTGTGAAAACGGCCGCTGTGTGAGAGTCCAG GAAGGATACACTTGCGATTGCTTTGATGGATATCACCTGGATATGGCCAAGATGACCTGTGTCGATGTAA ATGAATGCAGCGAGCTGAATAATCGGATGTCTCTCTGCAAGAACGCCAAGTGCATTAACACAGAAGGCTC CTACAAATGCTTGTGTCTGCCAGGCTACATACCATCCGACAAGCCCAACTACTGTACACCCCTGAACAGC GCTTTGAATTTAGACAAAGAAAGTGACCTGGAGTGAAGGAGAAGAAGCTGCGTAACCTATGCCCATATAC TCTGCACTGTGTAAAGGGAAAGGGAGAGATGTATACTTGAGACACTGCACCTAACCCAGAATGTGGCAAA GACAGAGACAACTTGGAGTTGAGTGAACCCCCAAAGACAGTGAGCGGATGGAGCTGGAACCTGACAGGTG CAACAGACCAAATGGACATTTCTTCAAAAACCCAGTATATATAGTCTGTTCATATGTAAAATTCAGTGGA AAAGAGGCAGGACATGGCTGTCATTTTAAACAGAGGTTGTATTATTATCTTGTTTTGATTTTTTTTTTTT ACTATTGCTTAAATTTGGCTTTTAAATATTAGTGGAAATGTTTTATGTGATCTTTCTTTTTGCTGTGCAG ATCCTCAGCTGCCGTTTTTTTTTTCCTCCAATGATAATCTCAAGTGCAAACATCTTTGATAAAATATTGT TAAACTGTATTATAAGTAATATGACACAGGGTTGTGCTGTCCCTTGGTCTGGAGACTTTTAAAAAAACAA GTTGTCTGTCCTGTGGAGCAGGCAGTAATTGTTAAACTTTTGGAGGGGATGGAGAAACATCTTTATTTCA ATGTATTACCAGATTGCCTGTCAATTGGATCCCTTACCTGGATGGAAGTGGAAGAGCTTCATAGAGTTTA TTAATATTGTGATGTGTAAAGTCAACCGAGAAAAAATTAAAAACCTGAACATCCCCCCCCAAACATTTAC CATTGTATGTTATAAGAAAATAAACTCACCATTTTTGTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AA
Notes:	The probe template was PCR amplified from IMAGE:30356631 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30356631 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13152 same embryo
	EMAGE:13153 same embryo
	EMAGE:13154 same embryo
	EurExpress:euxassay_017295 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source