EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:13178

Dgkk diacylglycerol kinase kappa ( MGI:3580254)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_017224. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_017224_01	euxassay_017224_02	euxassay_017224_03	euxassay_017224_04

euxassay_017224_05	euxassay_017224_06	euxassay_017224_07	euxassay_017224_08	euxassay_017224_09

euxassay_017224_10	euxassay_017224_11	euxassay_017224_12	euxassay_017224_13	euxassay_017224_14

euxassay_017224_15	euxassay_017224_16	euxassay_017224_17	euxassay_017224_18	euxassay_017224_19

euxassay_017224_20	euxassay_017224_21	euxassay_017224_22	euxassay_017224_23	euxassay_017224_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13178_wholemount_strong.wlz

13178_wholemount_moderate.wlz

13178_wholemount_weak.wlz

13178_wholemount_possible.wlz

13178_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13178_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9358
Entity Detected:	Dgkk, diacylglycerol kinase kappa ( MGI:3580254)
Sequence:	sense strand is shown >T9358 GGACCCCGAACCAGACTCAGAACCGGCCCCAGAACCAGTCTCAGAGCCGGCCCCAGAACTTGTCCCAGAG CTGGCCTCGGAGCCGGCCCCAGAACCTGTCCTAGAGCCAACCCTAGAGTTGGCCTCAGAACTGGCCCTAG AACCAGCCCCAGATCCTGCCATGGAGAGGGCCCCAGAGTCCTGGCCAGAGCCAACTCCAGAGTCCTGTCT AAGAATGAGTCCAGCACTACATCTGTTGCAGGTAGTTCCTGGAGAGAAAAGTGGAACAGCATCTCCAGTG CTATTGCCATTGCCGTCGCCCAAACCGCCAATAACGTGGTCCAGAATAAAGAAAATACTGAAGGAAGGAC CCCTGCTGAAGAACTGTAACTCCTTCAGAAGATGGAAGCTTAGATACTGTCTGGTTCAAGGGCAGAAGCT CCACTTTGCACACCATCCTTCGTTTGCACACTTTGAAACAATTGATCTGTCTCAAGTTGTTTTGGCTGAA AGTAGCTGCAGAAATCTTTGCCATGGTTTTTGTGTTATCACACCACATCGAAAAGTGTCTCTAGTTGCAC CCACTCGGCAAGACATGGAAGAATGGATTAACATCATAAAAACTGTCCAACAAGGAGAAATCCGTCAGAT CCCTGCAGCCGAAAACAACCCCTTTCTTGTCGGAATGCACTACTGGTATTCCAGTAGTAATCCCCGGAGT CACTTCTGCAATGTTTGTCGAGAGAACATTCCTGCTTTATCTCGAGATGCCGTCACCTGTGAAGTCTGTC AGGTGAAATCTCATAAATTCTGTGCTTTGAGAGCAAACAAAGACTGTAAATGGAATACTTTATCCGTGAC TGATGACCTTCTTCTACCCGCTGATGAAATACAAACAATGCCGCATCAGTGGGTAGAAGGAAATATACCC GCTGGCTCTCAATGTGTCGTATGTCACAAGAGCTGCGGTAGTCATCACAGACTTCAGGATTTCCGCTGCC TCTGGTGCGGCTCTACGGTGCACGGGGCCTGCCAGAAGCGGTTTTCCAAGGAATGTTCCTTCGGAAGTCG TCGCTCGTCCATCGTGCCACCAACGGCACTGAGCGACCCTAGGGGCGATGGCCAGCTAGTAGTATCGCCT GACTTCTGGAATCTTGACTGGCCACTGACTTGTTCCTGTCCCTTGCTCATCTTCATCAACTCTAAAAGTG GAGATCATCAAGGGATCATCTTTCTCCGGAAATTCAAACAATACCTTAATCCGTCTCAAGTATTCGACCT GGCGAAGGGTGGACCTGAAGCAGGCATCGCTATGTTCAAGAACTTTGCCCGTTTTCGTGTTCTGGTTTGT GGTGGAGATGGCAGTGTGAGCTGGGTCTTGTCTACGATTGATGCCTATGGATTGCATGATAGGTGTCAGC TGGCGATCATCCCACTTGGAACTGGTAATGATCTCGCTCGTGTCCTGGGCTGGGGAGCAGTCTGGAGTAA AGGCACATCACCACTGGATATTCTCAGCAGAGTGGAGCAGGCTCACGTGAGGATTTTAGACAGATGGAGT GTGATGATCCGTGAGACCCCCAGACAAGCCCCGCTGCTGAAAGGACAAGTGGCAATGGATATACCAAGAT TTGAGGCTGCTGCCATCAAGAACGTAGAGTCTGCAACCACTGAGTTGAACAAAATTCTGAAGGCCAAATA TCCCACGGAGATGGTCATCGCCACAAGATTCCTGTGCTCAGCAGTGGAAGATTTTGTGGAAGATATTGTA AAGGCCTGGCACCAAATAAAACAGAACAGCACAGCAGTGGAGTCTGTGATTTTGAAAAGCGACTTAATGT ATGATAAACTCAGTGTCCTTATTGATCTCCTGGCTGAGGATGCAGTAGCTGCCTCCGCTGAGAGGACAGC CACAGCATATGGAAGCAGAAGCCAAGCAGATGGAAAACCCTTCGTGCCTCAACTAGACCACATAGCAAGG GCCAAACTGGAGTTGGCTGAAAGGGCTCAGAAACTCCAGCAGTCCTTGAAACTCATCATATTCCAGGTCG AACAAGTTCTGGATGAAGAAAGCAGACAGAGCTTATCAGTTAAGAACTTTACTTCATCTTTGTTCCTGGG AGACGGAGACGACGACGACTCAGATGACTATGATCAGAGCCCTAGACACCGTTCTCGTTGTGACATCTTA TGTTCTATACCTTCTCTGAGAAATGAAGACCTAGATAACCTTGACTTAGAACACTTACACATTGCACCTG AAACGATACGCTTCAAAAAAAAGTGTGTCATGAACAACTACTTTGGAATTGGACTAGACGCAAAAATCTC TCTGGAGTTTAATTCCAGAAGAGAAGAACATCCAGAACAATACAATAGCCGCCTCAAGAACAAAATATGG TATGGTCTTCTGGGAAGCAAGGAATTACTGCAACGCTCTTACAGGAAATTGGAAGAGCGAATACACCTCG AGTGTGATGGAGAAGCTGTCTCCTTGCCAAACCTTCAAGGCATCGTAGTGCTCAACATTACGAGCTACGC TGGAGGCGTCAACTTTTGGGGAAGAAACAGAGCAACCACAGAATATGATGTCCCTGCAATCAATGATGGG AAGCTAGAGGTTGTGGCAATCTTTGGTTCTGTGCAGATGGCCATGTCTCGTATCGTCAACCTGCAACAAC ATCGAATTGCGCAGTGCCATGAGGTGGTAATAACTATTGATGGTGAAGACGGTGTCCCTGTGCAAGTGGA TGGGGAAGCCTGGATTCAGAAGCCAGGCCTTATCAAGATTAAATACAAGAATGTTGCCCAGATGTTGATG AGAGATAGGGACTTTGAGAACTCAATGAAAACCTGGGAATCTAAGCATACTGAAATCCAAGCTGTCCAAC CACCCCACCTGGATTTCCAGGAATCTCAAGACAGCCTCTCTGATGGAGAGTATGCCCAGATGCAGCACTT GGCTCGGCTTGCAGAAAACCTCATTAGCAGACTTACTGACCTAAGCAAGGTCCACCAGCACGTGTCTGTC CTCATGGATTCTGTGAATGCCAGTGCTAACATACTGAATGATGTGTTCTATAGCCAAGACAGTGGTAACG AAGCAGGCGCAGCTTCCTGCATTCCCATTGAGACTCTAAGCAGAACTGATGCAGTAGATGTTACATTTAG TCTTAAAGGGCTCTACGATGACACCAAAGCTTTCCTGGATGAAAACTTGTTGAGAGATGCTGAGGATCGG GCCATGCTACAAACTGCCTTGGATGCCATGAATACAGAGTTAAGAAGGATACTGGCAATTGGCTGGCTGA GTCAAATCTTCTTTCCAGAGGAACAGGCTTCTGACACTCGCAGTTTAAGTCGTAGGTTTAGAATAAAATT CCCCAAGCTGGGGAAGAAGAAACAACGAGAAGAGGGAGAAAAGCCTAAATCAAGCCAAAGATTCCCTGGT TTTCTTGGCAAGTTCTGGCGTCGAAGAAATCGTAGCAATCGAGCAAAAGCTGATGACCCTCCAACACCTT CAAGTTCTCAACTATAGTCCTTGAAAGCTGGAAGGAGAGCTCTCAACACAGAATTCTACTAAAAACTCTC AAGACCTCAAGAAAGGCTGAACTAAATTATTTCTGAACAAACATGAGCACCACCAAGAAAGACCCTCCAA ATCCTTATACTAATATGTTTTCCCCAGACTTAATCAGATCACCTTAGAGGGTTACATTTTTCTCGTTGGC CAAAGATTCTTGTTCCGAGTTGTCTTGTTCAGTTCTTCTCGTTGTGTACCTTGGGCAACTACAGTGTTTA TTGGGGACAGGCTCTCTATGAATGGCCTGGGAGCTATGAGAATGTATGGGAGTGCATCTCGTTAGGAAAT ATTAGAATGGGGGGTCTTTCACTTGGAGAGAAACTGTTGACACCAAGGAATAAATGAGTGACCAAGTCTC AGAAAGATGAGGAAGGGGAAGGGTGTCCAGGCTTAGTTGTGTTTCTCCTCCAATCCCTGCCAATTGCAAC TTGAATTTCAAAAGATGGCTCCTAGTTGGCTTAAGAAGATGAGAACAAGGTCCTGGGGCTTGTCCACTCA TCTGTTACAATGACTATGTGAATCTTTTCTTATAGATATAGAACTCTCCTCTTCTCTCTTTCCTTTCTCC TCTCATTTCCTCTTCTCTCCTCTACCACCTCTTATCTCCTCTACATCTCTTCTCTCTCCTCTCACTTCTC TCTTTCTCCACCTCCTTCTAATTTCTTTCCCCTTTCTTTCCTTTCCATCTCTCCTTCTCTTCCACTTTCC TCTATTACTCCTTATCTCCCCATAAACTCTTCGTGTTTTTCCATGTGTACATGTATGTCTTTGCACATGT CCAGGTGGTGCTCTCGTGGCTGGGTGGGCCCACTGGTTGGAACTCACCCCTCATGGCAACCATACAGGCT CCTGTACTTTTGCCTTGATCCCAGCCTGCATGTCTCCATGGAGTGCTTGCCTGCATTCTCGCTGGATTTT TTAACTAAATGTTTTGCCGCTGTGCTGCAGTATAGATTATATACAGAGACATAAAGATGTACATATATAT ACATATATATTTTTAAGAACTGAAAATACAACTCGACCTCTAAGTGACCCTCTAATTTATTATGATAACC CAGGAATCCCTCCTCATCCCTATTACCCTATACACATATATACTCTACCAACTGCCCTAGTTCTTGGTGG AGCTTCTAATCTTTTGGTGCATTTTTTAGTGTCTTAGAAGTAGCTGGGGTCTATATATCCCAGCTAATTC TGACATCCACTTCTGAGCCAGGCCACAGAAGGGGAACAAAAACACCAATGCCCTGCCAAAAGGGCCCAGC TGGCATGCTGCCATTTAGCTCTGTTTCTCCTGGCTCTACATCAGTAACTGCCCAAGCTATATCACCCTCC TCCCCAATAAAAAGATGTCAAAGAGCTCTACCCTTTAAATGTCTAAGTCAGGGTCTACCTGACAATAAAA CCACAGCCCATGCCCCTCCCCAAAAGCTTAATCTGGGCCTGCAGCTATTAGATAAGCTGTTGGGTCACTC CTCAAATTATCTTCAAATCTCATATCTTACCCCCTCCCCCCCCCCCCAGAGTATAAGAGAGGCAGTCAGA GTGACCAGAAAGAACATTGATCTGGGAAAAGAAAGGCGAAAAGCCTCTGCTTCCCCACTGTTCTGCTTCC AAGGTGGTTTTCATGAACATAGTCGGTCTCCAATCTCAGTCAAGTAAATCTGCCGAGAGTGCAGTGCTCA GAAAGAAGGTCCCTGGTCTACCTCCTCTTCACCCTCAAGTCGCACCTTTTGGAACAAGTCCCTTCCCACT TATCAAGAAAAAGATCCAGAAAACTGCCCTTTTCTCACTGCTCAGCCAAACTTAGCGTGCTCAATTACCT TGCTCAGTCCAATGGCTGGTTCACTGAGGTGCACAATCCAGACTTCGATCACTGGTTCTTAACTTTCTAG AACACCACCAGCCTAACCCACTTAAACCAGCTGCCTGTGTTCCACCTCTTCAAGGGTGAAAAAAAGAGAC TAACAATGGGGTAGTCCCCAAACATCTGATAGCTGTGCCAAACAAAAAAACAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6852516 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6852516 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13180 same embryo
	EMAGE:13181 same embryo
	EMAGE:13177 same embryo
	EMAGE:13179 same embryo
	EurExpress:euxassay_017224 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source