EMAGE mouse gene expression spatial database

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EMAGE:13214

St6gal1 beta galactoside alpha 2,6 sialyltransferase 1 ( MGI:108470)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_017335. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_017335_01	euxassay_017335_02	euxassay_017335_03	euxassay_017335_04

euxassay_017335_05	euxassay_017335_06	euxassay_017335_07	euxassay_017335_08	euxassay_017335_09

euxassay_017335_10	euxassay_017335_11	euxassay_017335_12	euxassay_017335_13	euxassay_017335_14

euxassay_017335_15	euxassay_017335_16	euxassay_017335_17	euxassay_017335_18	euxassay_017335_19

euxassay_017335_20	euxassay_017335_21	euxassay_017335_22	euxassay_017335_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13214_wholemount_strong.wlz

13214_wholemount_moderate.wlz

13214_wholemount_weak.wlz

13214_wholemount_possible.wlz

13214_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13214_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9308
Entity Detected:	St6gal1, beta galactoside alpha 2,6 sialyltransferase 1 ( MGI:108470)
Sequence:	sense strand is shown >T9308 CAGACTGCAAGATTCCCTGCATTACGGGGAGCCCTAAGCAGGCACCTTTAACCTTGAGCCTGCTCGCTAG CGACCTCCAGAGGGCCAGGATGGTTTGGGACCGGTGTGATAGAGCACTTTTCTAGTTTTAACGACTCTAT TTGCATAACGGAGACCAAGCGTCCTCCCGCCGGTCTGCCGTGCTTTTCTTCCCTCCTTCCCGGTCCTCTG TACCCTCATCTGTGCTCCCTCTCTGCTTCCCAGAAGATCCGTTCTCAAGGGGACTCACTCGGAGCTTGAG TCCCGGGCTCTTCTCCCTCGGCCCGGCAAGATCCAGAGGCACCTGGGGGAGTGGAGTTGAGCGCCAGCCT GGGTTGGGGAGCCGCCCTGGCACTGCCCGACCCTAACAAAGGGAATCGGACTGCGCGCCGTGCGAGCGCT CGGTGGGCTCAGCGACCGCAGCCGCCTTCCCGATTGAGAGAGCTGCAAGCAGGGGTAGAGCCAGCTGTGC CAAGCTCCACAGCCAGCGCCCGAGAGAGAAGTTATCAGTGCCCCGGAAGTCAACTGAAATGAACTGGCCC CCTTGAGCCTTCCCCAAATACCTGGTACCCACTTTCCAGCCGGATGCTGAGATGGTTTTTGATCATCCTG TGAAAAATGAGCCTTGGCCTCCAGACCTAGTAAAGTAAACCTCTTTCCCATGGAGAACAGTGCTGGCTCC TGAGGACCTGGAGGGCCCAAGCCCCAGAAGGATTAGCCAGAAGCAGGCTTGGTTCCTGCTCCGATCAGTG GCTCTCCTGCCCGTACCATTCATTATGATTCATACCAACTTGAAGAGAAAGTTCAGCTGCTTTGTCCTGG TCTTTCTCCTGTTTGCCATCATCTGCGTGTGGAAGAAAGGGAGCGACTATGAGGCTCTTACATTGCAAGC CAAGGTATTCCAGATGCCGAAGAGCCAGGAGAAAGTGGCCGTGGGGCCTGCTCCCCAGGCTGTGTTCTCA AACAGCAAACAAGACCCTAAGGAAGGCGTTCAGATCCTCAGTTACCCCAGGGTCACAGCCAAGGTCAAGC CACAGCCCTCCTTGCAGGTGTGGGACAAGGACTCCACATACTCAAAACTTAACCCCAGGCTGCTGAAGAT CTGGAGGAACTATCTGAACATGAATAAATATAAAGTGTCCTACAAGGGGCCGGGACCAGGAGTCAAGTTC AGCGTAGAGGCGCTGCGCTGCCACCTTCGAGACCACGTGAATGTGTCTATGATAGAGGCCACAGATTTTC CCTTCAACACCACTGAATGGGAGGGTTACCTGCCCAAGGAGAACTTCAGAACCAAGGCTGGGCCTTGGCA TAAGTGTGCCGTCGTGTCTTCTGCAGGATCTCTGAAGAACTCCCAGCTGGGTCGAGAGATTGATAATCAT GATGCGGTCCTGAGGTTTAATGGGGCACCTACAGACAACTTCCAACAGGATGTGGGCACAAAAACTACCA TCCGCCTAGTGAACTCTCAGTTAGTCACCACAGAAAAGCGCTTCCTGAAGGACAGTTTGTACACCGAAGG AATCCTGATTCTGTGGGACCCATCTGTGTATCATGCAGACATTCCGCAGTGGTATCAGAAGCCAGACTAC AACTTCTTCGAAACCTATAAGAGTTACCGAAGGCTTCACCCCAGCCAGCCTTTTTACATCCTCAAGCCCC AGATGCCATGGGAACTATGGGACATCATTCAGGAAATCTCTCCAGATCTGATTCAGCCGAATCCCCCATC CTCCGGCATGCTGGGTATCATCATTATGATGACGCTGTGTGACCAAGTTGATATTTACGAGTTCCTCCCA TCCAAGCGCAAGACAGATGTGTGCTACTATCACCAGAAGTTCTTTGACAGCGCCTGCACGATGGGTGCCT ACCATCCGCTCCTCTTCGAGAAGAATATGGTGAAGCATCTCAATGAGGGAACAGATGAAGACATTTATTT GTTTGGGAAAGCTACCCTGTCTGGCTTCCGGAACAATCGCTGTTGAGCCAGGCATCCTCATCCTTCTCCA TAAGTCATTTTATGGCTGCTCTCCTGGTTACCACTGCTTGAAGGAGTGTTTTTATTCAACAGGCCCAGCC TGCTTCTTGCACTCTAGGGAATTTTGTTGGCGAGGGTTCTGGGGCCTCCAGTGTCTGATGGCAAGGAGGC AGACCCAGCCTTCCCTGCAGGCACAGAGTATGGGAGCCCAGATTCTTGCCGTACACATTTCTCCTGACAA CATCCTCCTCTCCTTTCACATGAGTAGGGGAAAGAGCCACATTTCTCACCAGGGTTGCCAAAACTAGACT TTGTTTTTTCCAACTGAATGATGTCATCCTAACAAAGGAGCACCTTCCGTGGCTTAAATCTCTCCCTAGG TGGTGATTTCACATCTCCCAGATCATGATTTGTGTCAGATGCAGGGTGGCTTCAGGGAGGGGAGGCAAAT AATTGGGACACGGTGGGGAAGCCTACTCAGTTACTCTAGAGTAAAGTTGTTCCCAAGGGTAGTCCCTTTT GATCCTTAATCCTGGGTGCTTATGATCTTGCAAAGGATCTTGGGTAGCTCACTTCCCACTCTCCATGGAG ACAGAAGGTACAAAACCTCCCATCTGTCCCCAACCTGATCAGAGTTGAGTAAAAGAGAGAGACACCCAAC AGGCAGCTAGAATGCAAACATTCCGTGCGCTCAGTGATTTGGGCTCCTCTCTGAGAGCGTGCTGTCTGTA CACCCAGCAGGGGCCAGTTCTACTGTGTTCTTGTTGAGCAAGAGCACTGGGGAAGCTCTTAGAGGAAGAA GAGAAACACACCGTCTTCTTCAGAAGAAGTTTTCACTTTCCCCTTTATCACTGATGTTAGAACATGGAAA CCCAAGGTATTTTTGTTAGAAAGTCCCTACCCACATTGGCACCAAGTGCTGCCCCACAGGAGACAGCAGA TTAGTCAGATAGGCTGGGGCTCTTTTATTTTATTTTATTGTTTTAGCTGTCTACTCCCTAACCTTCTCCT AATTGCTATCATTACTCTCCCTTACAGCAGGCTTCCCTCCCAAATTACTTTTAATCATTTTCTGTTTTGC AATTACCCCAACTTTCGCTGACCTTATGGGCCCCCCCGCCCCCCATGAAAGGTCTCTCAGGGCATGGGGA ATATGGTTTCTGGGACTTAGGTTAGAATCTTAATCATAACTGATTCTCACAAGGGGTGAGGTGTGTGATC ACAGATTGCCCTGCTCCCCCTGCAAGGGAGTAGCATTGGTCTCTGGGCAGGGAGGACCCACCCTGTTTGG CAAGCATACTCTCACAAGGATTTTCTGAGACCTCTCTACCTGGTGGGAAACCACAGCAGCCCACTCAGCC CCTGGTGTGGAGTGGTTCAGTGACTTACAGATCAGAATTAATGCTATTGAATCAAGGTCACTGAGACACA GTCTACTGGATTCCCAAGGCTGTGCTCACAGGGCTTCGGTGTTTCGAGCTGCAGACCCACAGTCTCAGAG CTCAGAAACTTGTTTACTGCTGACCCCCTTGCCTTATGCGGGCAATAGAAAGCCCGGAGTGAAAAGCCCT GGCTTTCCAGGTTGACCCCCACTTCCTCACTGTGCCACTTTGTTTCCATATCTGTAAAATGGGGGTGACA ATCCTACCTCACAAGGCTGTGTGGGGACCGCAGGAAAACATATATCTGGGCTGTGTGAGGACCACTGGAA AGCACGTGGCTGGGCTGTGTCCACGGTGCATAGGAAGTAGGTACCCTGATGTCTTTCCTTGTTTCCTTAT GGAGGGTCACACCCATACTGGGCCTTGCTGCAGGAGAGGGCAGTAGAGAAAAGCAGGGCATCCTTTCTGT TGTGAGGCATCTGCATCATTTGCAACGTGCAGGAGCCTACCTGTCTTGCTGCCTGCACCTCAGGGTGTGC AGAGCATTGGGCTGCTTGGCCGCTGTGGGGGTTAGGTCTAGGGAATTCCTGGCTCATTATAAGGCATCAG GACTGTGGATAGAGTGCCCAGGAAGCTGATGTTCCTTCAAGAGGTCAGCAAGAGGTACATTAGAGATGGA GACTACACTGGGTAGATTCTAGTTTTTAGTTCTTATTAATGTGGGGGAGTAAATTAATAAGTGTAATATG ATTCTGATGTCTGTTAGACTTTCTCTGTGCTCTGTAAATGTGGATACTTGAGTAAAGTGGACTACGGAGT GGATGTGGGCGTCACTGTTAGTCTGATGAGGTATTTAGTATTTGCGACAGGTAGTTTAAAATCTGTGACT GAGTGCTATAGGAAAGTGTCTTTCTACGTGGGCACTGACGTCACTAGCCATCTATGCATCCTAAATGCAG GATCCTCTTGATTCCTTCTGCCAATCAAATCTCTCATTGCTCTTGGTTTCCGGGTTTGTGTCAGAAACTT TTAAAACATACCTGTTAATTCTAAATTATCCAAAGATTATGTGCAAATTTTAAAATAAATATCTTTTTCA GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6844069 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6844069 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13218 same embryo
	EMAGE:13220 same embryo
	EMAGE:13215 same embryo
	EMAGE:13216 same embryo
	EMAGE:13217 same embryo
	EMAGE:13221 same embryo
	EMAGE:13219 same embryo
	EurExpress:euxassay_017335 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source