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EMAGE:13278

Dnajc25 DnaJ (Hsp40) homolog, subfamily C, member 25 ( MGI:1919679)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:13278 EMAGE:13278 EMAGE:13278 EMAGE:13278 EMAGE:13278
"Pseudo-wholemount" of euxassay_017459. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_017459_01 euxassay_017459_02 euxassay_017459_03 euxassay_017459_04
EMAGE:13278 EMAGE:13278 EMAGE:13278 EMAGE:13278 EMAGE:13278
euxassay_017459_05 euxassay_017459_06 euxassay_017459_07 euxassay_017459_08 euxassay_017459_09
EMAGE:13278 EMAGE:13278 EMAGE:13278 EMAGE:13278 EMAGE:13278
euxassay_017459_10 euxassay_017459_11 euxassay_017459_12 euxassay_017459_13 euxassay_017459_14
EMAGE:13278 EMAGE:13278 EMAGE:13278 EMAGE:13278 EMAGE:13278
euxassay_017459_15 euxassay_017459_16 euxassay_017459_17 euxassay_017459_18 euxassay_017459_19
EMAGE:13278 EMAGE:13278 EMAGE:13278 EMAGE:13278 EMAGE:13278
euxassay_017459_20 euxassay_017459_21 euxassay_017459_22 euxassay_017459_23 euxassay_017459_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:13278Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
13278_wholemount_strong.wlz
13278_wholemount_moderate.wlz
13278_wholemount_weak.wlz
13278_wholemount_possible.wlz
13278_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:13278_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T40259
Entity Detected:Dnajc25, DnaJ (Hsp40) homolog, subfamily C, member 25 ( MGI:1919679)
Sequence:sense strand is shown

>T40259
GCTGGATCACCCAGAAGAATACTATAGCCATTACTACCACTACTACAGCAGGCGCCTGGCCCCAAAAGTG
GACGTTAGGGTGGTGATTTTGCATTCCCAAACATCGCACGGTCCTGCATCCCCGGAGGAGCCGCTGCTCA
CATTCAAGGAGATAGAGGAACTCTTGTATTTCAGCTGGTGGAATAGCTACAATAAAGCAATCAGCTACCT
AGCCACAGTGCCCAAGTACCGCATCCAGGCAACAGAAATCGCCAAGGAGCAGGGACTGCTCAAAAAAGCC
AAAGAAAAAGGGAAAAACAAGAAGTCCAAAGAAGAAATCCGTGATGAGGAGGAGAACATCATAAAGAACA
TTATAAAAAGTAAAATAGACATAAAGGGGGGCTACCAGAAACCCCAAGTTCGGGACCTCCTCTTGTTTCA
GGTCATCCTCGCCCCTGTTCACCTGTGCTCTTATATAGCCTGGTATTGTAGGTGGATCTATAACTTTAAC
ATCAAAGGCAAAGAGTACGGGGAGGAAGAGAGACTTTACATTATCCGGAAGTCTATGAAGATGTCCCAGT
CTCAGTTTGACAGCCTGGAGGACCATCAGAAAGAAATGTTTCTCAAGCGGGAGCTTTGGATAAAGGAGAA
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 77952. Forward Primer - name:077952_F_cDNA_2010203O07Rik, sequence:GCTGGATCACCCAGAAGAATAC; Reverse Primer - name:077952_N_SP6_cDNA_2010203O07Rik, sequence:TTCTCCTTTATCCAAAGCTCC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:13277 same embryo
 EMAGE:13279 same embryo
 EMAGE:13280 same embryo
 EurExpress:euxassay_017459 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS