EMAGE mouse gene expression spatial database

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EMAGE:13319

Zfp169 zinc finger protein 169 ( MGI:1915161)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_017546. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_017546_01	euxassay_017546_02	euxassay_017546_03	euxassay_017546_04

euxassay_017546_05	euxassay_017546_06	euxassay_017546_07	euxassay_017546_08	euxassay_017546_09

euxassay_017546_10	euxassay_017546_11	euxassay_017546_12	euxassay_017546_13	euxassay_017546_14

euxassay_017546_15	euxassay_017546_16	euxassay_017546_17	euxassay_017546_18	euxassay_017546_19

euxassay_017546_20	euxassay_017546_21	euxassay_017546_22	euxassay_017546_23	euxassay_017546_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13319_wholemount_strong.wlz

13319_wholemount_moderate.wlz

13319_wholemount_weak.wlz

13319_wholemount_possible.wlz

13319_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13319_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9477
Entity Detected:	Zfp169, zinc finger protein 169 ( MGI:1915161)
Sequence:	sense strand is shown >T9477 CGGTTCTGGTGAGCGAAAGTGCAGACGTCCTCGGCTCTTGCAGATCCCTCCAGGCGGGGAAGAGTCCGGA GAGCTGGCGTGGGCCACCCACAAGGTTCAGATTGGACTCTGAAAGCAGACTCCTCTCCAGGGAGAATATT CTGGAACGTGGGAAATGGCCCCAGGGCTCCTGACAACCAGAGACAAGGCATTAATGGCCTTCCATGACGT GGCTGTGGCCTTCAGCCAGATGGAGTGGGAGCTGCTGAGTCCTGCCCAGAGGATCCTGTACAGGGATGTA ATGCTGGAGAACTATAGCCACCTTGTCTCCCTGGGAATTGCCTTTTCCAAACCAAAACTCATCACGCAGC TGGAACAAGGGGCAGAACCCTGGAGAGATGGCAGCGGATGCTTTCTGGACCTTTGTCCAGAACCCAGGAC AGAATTAGAGCCCTCTCTTTCGTCCCCGGTATCCTTTGCTAGTCCGGAAGTCCTCAGAGTGTGTGTGCTG AATGGACCTCCCTCTCAGACTTTTCCAAGCCCACCAGCAGGGAGCAACTTCCAGTCAGAAGCTCCAGGCT GTTCCAGCAATGAAGGAGGTGAAGAGAGAGACAGCAGCGATGCTCTGTTGCAGAGGACAAGTCCAACGAG AATTCTGAAGACGTTCTTTAGCCCATGTCAAGATCAGGCAGCATACTGGGTAGAAGGCGACAGTAAACAA ACAGACCTTGGCCTGGCCCCTGAGGAGGCAGTCACAGTGTTGAAGGGACCCGATGAGTCAGCATCTGGGG CCGTCCTACATGGAGCATTTCCGCTAGGGCTTGATGATGTCACAAACCTGTTCAGCCCTCAGAAGCACCA CGTGTTTCCTGACTGCGGAGGGGGCTTTTGCCAGAGTTCTGACCTCATGAAGCTCCAGAGAATGGACGGA GTGGAGAAGCCTTACTCGTGTTCGGAGTGCGGGCACCAGTTCGCTCAGAAGGCGTCTCTCACGGTCCATC AGAGGAAACACTCTGGAGAGAAGCCGCACGTGTGTGAGGAGTGTGGGCGAGGCTTCATGTATGTATCCTC CCTCAACAGCCACAAGAAGATTATCCACTCTGGAGAGAGCCCCCTTGTTTGTCCCGAGTGTGGGCGTGGC TTCCGCCAGAAGATAGACCTCCTCCTCCACCAGCGGACTCACCTGGATAAAAAGCCCTTCGTGTGTCCCG AGTGTGGGAGGGGCTTTTGCCGGAAAGCCTCGCTGCTACAGCACAGCTGGTCACACTCGGGTGACAACCC CTTCATCTGCTCGGTGTGTGGGCGTGGCTTCAGGCTGCTCTCCCGGCTCCTCACTCACCAGGTCACACAC TCTGGTGAGAAACCTCACGTTTGCCCGGACTGCGGACAACGCTTTGGCCAAAAGGGTACGCTCATGAGAC ATCAGAAGACACATACCGGGGAGAAGCCTTTTGTGTGTCGCCAGTGTGGCTGGAGTTTCACCCAGAAGGT TAACCTCATTAGGCACCAGAGGATTCACACAGAGGTAAAACCTTTCCTATGCCCTGAGTGTGGACGAGCC TTTGAGTTTAAGTCGCTCCTCACCAGACACCGTAAAACGCACATGGGGGAGAAGCCCTACGTGTGCCCCC AGTGTGGTAGGGGGTTTAGCCAGAAGTTCAACCTCATCGGGCACCAGAGGATTCATACAGGGGAGTTACC TTTTCTGTGCCCTGAGTGTGGGCGTGCGTTTGTAAAGCAGTCCATGCTCATCAGACACCAGAGGACCCAC GCAGGGTGGGAGAAACCTTACCAGTGTCCCGAGTGTGGACGCACCTTTGAGTTTAAGTCGCTCCTCACCA GACACCAGAAGATACACACGGGGGAGAAGCCTTATGTGTGTCCTCAGTGTGGCAGAGGGTTTAGCCAGAA GTTCAACCTCATTGGGCACCAGAAGACACACACGGGGGAAAAGCCCTATGCCTGTCCCCAGTGTGGCCAG CGCTTCAGGCAGAAGTTCAACCTCGCTAGGCACCAGAGGATTCACACGGGGGAGAAGCCATTCCTGTGTG CTGAGTGTGGGCGAGCATTTGGAAAGAAGGTCTCTCTCATTAGACACCAGAGGACCCACGGAGAGGAGAA GCCTTACCAGTGTACTGAGTGTGGTCGAGCCTTTGAGTTTAAGTCTCTTTTCATCAGACACCAAAAGACA CATACGGGGGAGAAGCCCTACGTGTGCCCTGAGTGTGGCAAGGCATTTAGCCAAAAGTTCAACCTTATAG GGCACCAGAGGAGCCATACAGGGGAGAAGCCTTACCTGTGCCCCCAGTGTGGGCGTGCCTTTGCTTTTAA GTCACTTCTTACTAGACACCAGAGGACACACTCCAAGTAGAAGCATTTTGTAAACAGCCTATGTAGGTAG GGACTGGATGGGATGACACCTCATCTCTGACTAGAGGACCCAGTCCACTCCAAAGAGAAGCCATTGGTTT GGGCACGGCGTTGGCTTTAACTCTGCCCTCATCAGACATCAGCAGACTTACTCAGGCAAGAAGCCCTAAG AACGGGCATTGTGGGCATGGCTTCAGCTGGAAGTTTCACTTTCATAGACATAGGAAGGCCAAGTATGGCC ATCAACTTCTAAGCCTGAGAGGTGTTTGCCTGACTCGTTTCTTCCTTTTTCCAGGAGCATGAAACTGACA GAAATCACTAGTAAATGTTTCTGGAAATGGAGTAGGGGAAAGCCCTTACTTGTTCATGAAATCATTGGTT TATAGTTTACTATCTTCAGTTAGCAGTATGCGTTAGTTTTCTAGGGTTTTCTAACAAAATACCCCAGACC ATAGAGCTCAGGCAAAAGAAGTTTACTTCGCCATATATATGAGCCTAGAAGTCCTCTATCAGGCCGGCTC AGGTGTTGCCGTGACTACACCTCCCCTTAGCAGATGGATGGCTTCCCGATGTGCTGTGCACCTACAGGTC CATCCCTCCTGTATATACTTTTCCTGGTGCCTCGAATTCTGGATAGGACCACATCCAGATGACCTTGTTT AACCTTTATTAAATGTCCTGCCTCCAAGTCACGCCACACTAGAGGTAAGGTATCGGTATTTATGTTGTGG GGCTAGGATTCCCAGAATCCTCAGCACCTTACATCCTTAACTCATTTCAGCGTCAGCTCTAAATTGAAAA TCTCTTAATACAAATATCTAATTCATGTATGGGTGAGGATATAGTTCCAGTTCACCTGTAAGCCGTATTT CTATCAGCAATCCTATGAAGTTAAACATGGAACTTGCTTCCAAAGTCCAATGACTAGACAGATACAGGTT AGATATTCCTATTCCGAAAGGGAAACATCAGAAAGACCTGAGCAGTGATGAGTCCCAGGGTCCAGCACAA ACCTAGCAGGCAAATTCAATGGGACCTTAAGTCTCAAGAGTAATATTGACTCAGTGTTCTGTCCCCTGGC TCACAGGACTGGCAGGCAGTGTTGTTTTCCTAGCCCTGAACAACAGTTTCACACGTTTGACCTCGCTCCA GACCTGACCAGGGTTTGGAACCACATTTTCCTTTTTTCTATTCTCTCTTTTCTTTTCTCTCTTCTCTTTT TCTCTGCTTTTCTTTTTTCATTTCCCTTCTCTACTCTTTTCCTTTCCTCTCCTCTCCTTTCATTTCCTTC TTCTCCTTCCTTTCTCTCCTTCCTTCCTCCTTTTTTTTTTTTCCTTTTTCTTTTTTTTTCTTTTTTATTC TTATTCTTATTCTTCTTTTTCCTTTTGTTGTTCTGTTACATACCCTGGCTATCTTGGAACTCAATGTGTA GACCAGGCTGGCCTCAAACTCAGAGATCCACTTGACTGTGTTACTGAGTGCTGGGGTTAAAGGGGTGAGC AGCCCGAGAGCTCTTCCCTTTTCCTGGAGATGAACAACAAACTGCTTGGAGCTCTACTGGTCTTCTCTTT AGAAACTCTGAAGTCCAAAAGTCTCTCTTCATTTTACCCCAGTCTCTTTAAAGCTCTCTGTCCCCCAGGG CTAGAGGAGATGGCTAATTAACTCTGGTTCACACTAATGCTAATCTCTGTTGAAAATAGTTTCCAGCCAT CTACTATTCCCTCCACACACACACACTTTTGTGTGTGTGTATATATATTTATTTTACAGTATGAATAAAG GGAGAATTTTCCAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30356624 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30356624 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13320 same embryo
	EMAGE:13322 same embryo
	EMAGE:13321 same embryo
	EurExpress:euxassay_017546 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source