EMAGE mouse gene expression spatial database

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EMAGE:13402

Gpbp1l1 GC-rich promoter binding protein 1-like 1 ( MGI:1924360)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_017401. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_017401_01	euxassay_017401_02	euxassay_017401_03	euxassay_017401_04

euxassay_017401_05	euxassay_017401_06	euxassay_017401_07	euxassay_017401_08	euxassay_017401_09

euxassay_017401_10	euxassay_017401_11	euxassay_017401_12	euxassay_017401_13	euxassay_017401_14

euxassay_017401_15	euxassay_017401_16	euxassay_017401_17	euxassay_017401_18	euxassay_017401_19

euxassay_017401_20	euxassay_017401_21	euxassay_017401_22	euxassay_017401_23	euxassay_017401_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13402_wholemount_strong.wlz

13402_wholemount_moderate.wlz

13402_wholemount_weak.wlz

13402_wholemount_possible.wlz

13402_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13402_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9255
Entity Detected:	Gpbp1l1, GC-rich promoter binding protein 1-like 1 ( MGI:1924360)
Sequence:	sense strand is shown >T9255 TTTGTTATGGTTGTGGGCTAGTCACCTCTGCTGGTCGGAGAAGCTGAGGACGCGTCCTCAGACGCCGCGA GTCGTCCTTGACCGTAGCCCCGCCGCTGCCGGAGATTGGCAGACTCAAGTGGCCTTTTTAATGTTTGAGT AATGATGGAGATGGACCCAAATTAGAAAGTGATTTCTTCAATAAAGCAATAGAAGATTCTTCCTATCTTC TGGAAGCTCAGTCCAGTTTTGGGGAAGGAGGAGAACAAGTGAAATACAGAAAATTGATAAGAAGCAAACC TCATTTTGGAATTCTCTTTTCTTCCTGACCCTTATCTTTTCTACTTATAATTTTGCTTTGAATGTATAAA TAGTAAAGACCAAAGAAATAGGGATATATATTTAGGAGCTTTATACCAAGAAATTTGCCTTGAAAGCTGC TATTCTGTGGCAGGAAAACTATATCAAGTTTCTATTGGATTCCTTTTTCAAAAAACAAAACAAGACTTCT TATATCTTTAGATTTTGAAGGTTAAAACTATAAAAGCACATCTCTCTGCACAATGCTTTAAGCAGTTGTG TGAGATAGTGAGATTTATGTTAAAAGCTTCTGTTGTGGATAGTTTGCCACTGGAATTGATTGTGCAGAAG CTCTCTTACAGTGCTTTACTATTACATTTGACCTGTCACCTTGATGACATTTCTTGCCCACATCTGAGAC CATTTGTCCTTGTAGCCATGACCTTGTTATGAACAGTGCTTGCACTCACTGTGCAGATGTCCATGTTTTG CCTATTTGAGCAACATTACAGTGCCATTACTCAAGGCAGTTGAATGAGGTGAACATAGACATAGCTTCCC CCCTTTCCCCCCTTTTTTAAATGTAACAAATAACCTTTTATGTTCCCCTTCCCCCCCTTCCCCTCCCCCT CCCCCCTTTGGAAAAGTGTTGGGAAGGAGGTATTGAGGATGAGCCGTTGAGAGGATTAAGAGGGTGGGCC ACACAGAGCTGGGCTTTTTTGTGCTTCATCACAAGGCGTGCTGCTGGCCAGGCTATTTTAGCACCCTTCT TACAAGAACACCTCGTGGAAGATACAGGCGGCAACTTTGGATACAGGACCACAGAGGTGACACTCTGAGG ATCCTGGCCATGAGGTTGGATGCCTCATCTTGCTAAAAAGACACTGGACCTAAATGGCGCAGCATGACTT TGTTCCTGCTTGGCTAAATTTCTCAACGCCACAGTCATCTAAGTCATCTACAGCCACCTTTGACAAACAC GGAGAGCACCTATCCCGTGGAGAAGGTAGATTTGGAATAAGCCGTCGTCGACATAATTCCTCTGATGGTT TTTTTAACAATGGACCTCTACGGACTACGGGAGATTCTTGGCACCAACCCTCCCTATTCCGCCATGATTC TGTTGACTCTGGTGTCTCTAAGGGAGCATATGCTGGAACCACAGGTTGGCATGGCTCTTCCCGAGGTCAT GATGGTATGAGCCAACGTAGTGGAGGTAGTAGTACAGGAAACCACCGACACTGGAATGGCAGCTTCCATT CCCGGAAAGGCTGCGCCTTTCAAGAAAAAACACCAACTGAGATTAGGGAAGAAAAGAAAGAGGATAAAGT AGAAAAGTTGCAGTTTGAAGAGGAAGACTTCCCTTCTTTGAATCCAGAAGCTGGCAAACAGAATCAGCCC TGCAGACCTGTTGGGACCCCTTCTGGAGTGTGGGAAAATCCACCTAGTGCCAAGCAACCCTCAAAGATGC TAGTCATCAAAAAAATTTCCAAAGAGGATCCTGCTGCTGCCTTTTCTGCTGCATTCACCTCCTCAGGATC CCACCATGCAAATGGGAACAAAGTGTCAACAATGGTCCCAAGTGTCTATAAGAACTTGGTGCCTAAGCCT GCACCACCTCCCTCTAAGCCCAACGCCTGGAAAGCTAACAGAACGGAACACAAACCTGGATCACTTTGCT CTAGCCGGGAGTCTGCTTTCACCAATCCAATTTCTGTTACTAAACCAGTGGGACTGGCTGCTGGTGCAGG TCTCCACTCTCCCAAAGAGAGTCCCTCCAGTACTACCCCTCCAATTGAGATCAGCTCCTCTCGTCTGACC AAATTGACCCGACGAACCACAGACAGGAAGAGTGAGTTCCTGAAGACTCTGAAGGATGAACGAAATGGAG ACTGCTCAGAAAGTAGAGACTGTGACAAGCTGGAGGGGCTAAGATTGGAGGGCAGCCACACACCCGAACC AAAGGAAAATGGAGAACAAGGTTGTCTTCAGAACGGTCTTTCTCTACCCATGGTAGAGGAAGGGGAGGTT CTGTCACACTCTCTGGAAGCAGAGCACAGGTTATTAAAAGCAATGGGGTGGCAGGAGTACCCTGAAAATG ATGAGAGCTGCCTTCCACTCACAGAGGACGAGCTCAAGGAGTTCCACCTGAGAACAGAGCAGCTACGAAG AAATGGGTTTGGAAAGAATGGCCTCTTACAAAGCCGTAGTTGCAGCCTGTCCTCCCCGTGGAGAAGCACT TGCATTGCAGAGTGTGAGGACTCAGACTCGGAAACGAGCAGCAGCCAGACGTCAGACGACGATGCCTGGA AGTAGAGATAAAATGCTCACAGTGACCTGCCCCGCTGAGCTCAGCCTTGTGTTTAGAGAATATAAAGTGT TCTTTTCCTTTTCTTATGTTGTTGAATACTTCATGCACAAGGGAAATAATCATATCCCAAAGAGAGAGCA ATTGTCTTGCTTAGCCTTTGTTTTCTTCCCTGTTACCTGCTTAATAGGCATTTGTGTGGTGGGATTTTTT TAAGCACACACACAGTACCTGTGGGGCTGTGCACAAGTGGGAGCTGTCAGGGCAGAGATGCAGGGAGGAG ATGCTGCTCTGCTCAACAGCTTCTACTACCAGCCCTTGGGGACTCACCCCTGTGCTCAAGCAATCATTGT CAATGACAAAGTGACTATTGAAGTTATAATTGTATTAAATTAATGCTAATAATTTGGATATTTTATTTTA TTTTTGGCTGCTGGGTAACTTAGCCCTTAACCAAGCATATGTGGTTGTTTTTGTTTTTTTTTTTTTTTTG TTTTGTTTTGTTTTTTATTTTCTCTTTTTTTTTTTTTTTCTTTTGGGTACAGCTGTAAAATATTTGGATA TAGGAAATGTTATTCTTGCAGCCTTGATATTCAGGGTGGATTGTAAAATATGAATTTTTGTGAGATTTCA AAGATTAAGATTATTTTGATAACATTATTTACAGATTTAAAAGATGTGGTTACCACAAGTCTGTTGAGGG GAAAAACTACTGCATAAAATAACTAACGTCGAATAAATATTTTGCATCAGTTTGGATTGTCTTGTGTAAG AAATATATTTTCTTTAAAATTAGAGAGTGGATTCTTTTTTTAAGGGAAATATTTTTAGAAAACTAGGGGA AAACTGAATTCAGCCCCACCTGTTTCTCTTTTCAGCAAAAATTCTTAATGTGGAAAAAACCTGTGCAATA AATTATTCATAATAAACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6836605 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6836605 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13400 same embryo
	EMAGE:13401 same embryo
	EurExpress:euxassay_017401 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source