EMAGE mouse gene expression spatial database

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EMAGE:13418

Mettl7a2 methyltransferase like 7A2 ( MGI:3026615)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_017543. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_017543_01	euxassay_017543_02	euxassay_017543_03	euxassay_017543_04

euxassay_017543_05	euxassay_017543_06	euxassay_017543_07	euxassay_017543_08	euxassay_017543_09

euxassay_017543_10	euxassay_017543_11	euxassay_017543_12	euxassay_017543_13	euxassay_017543_14

euxassay_017543_15	euxassay_017543_16	euxassay_017543_17	euxassay_017543_18	euxassay_017543_19

euxassay_017543_20	euxassay_017543_21	euxassay_017543_22	euxassay_017543_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13418_wholemount_strong.wlz

13418_wholemount_moderate.wlz

13418_wholemount_weak.wlz

13418_wholemount_possible.wlz

13418_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13418_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9993
Entity Detected:	Mettl7a2, methyltransferase like 7A2 ( MGI:3026615)
Sequence:	sense strand is shown >T9993 ACTCGCAAAGATGACGCTTGCCGTCCAGGCCATGCGGCTGGTGGTCTGCATCGTGGCGTTTCCCATGTTT CTGCTGAACCTTCTAGGCATGTGGAGCTGGGTATGCAAAAAGTGCTTTCCCTACTTCCTGAAGCGGTTCG CCATGATATACAATTGGAAGATGGCGAGCCTAAAGCGGGAGCTCTTCAGCAATCTGCAGGAGTTCGCCGG CCCCTCGGGGAAGCTAACTCTGCTGGAGGTGGGCTGCGGCACCGGGGCCAACTTCAAGTTCTATCCCCCC GGGTGCAGGGTCACTTGTATCGACCCCAACCCCAACTTTGAGAAGTTCTTGTTCAAGAGCGTCGCAGAGA ACCGGCAGCTGCAGTTCGAGCGCTTCGTGGTGGCAGCCGGGGAGGACATGCACCAGGTGACCGATGGCTC TGTGGACGTGGTGGTCTGCACCCTGGTGCTGTGCTCGGTGAAGAACCAGGAGAAGATTCTGCGCGAGGTG TGCCGAGTGCTGAAGCCGGGAGGGGCTTTTTACTTCATAGACCATGTGGCAGATGAACGGTCTACCTGGA ATTACTTCTGGCAGCAGGTCCTGGCTCGTGTGTGGTTCCTTGCTTTTGACGGATGCAATCTGACCAGAGA GAGCTGGAAGGCCATAGAGCAGGCCAACTTCTCCAAGCTGAACCTGCAGCACATCCAGGCCCCGCTCCCC TTGACCTTGGTGCGGCCCCACATCTATGGCTATGCTGTGAAATAGAGTGAGGTGGTGGGAGAGATCCGGT CTGCTCCACGCTGGTTCCAGGCTTCCGTTTTAGATGTAAAATTCTCACTGGGAGGGGTGAAAATCAAATT CATCCCTACAGGTTTCTGTTTAGCCATTTTCTATGTTGTTTCTTACCGAATCAAAACCAAATCAGAGCAG CTTTGAACTCTCCCTCCAAAGGGACCGAAGGTCTCTCATAGGCCTTTGAAAAGGAAGCAGTGGGGGAGAC TTACAGCTTGCAAACCGAATAAATAAATCCCAGAAAATGGAAAATCCACGGCTGACTAACACAGGAACAC CTCCTTCCCTCCCCCATCCCGGTAAGAAGGAATCAGAACCCACTTGCTATCCTGGTGTTACTAGTCTGCT TTGGCTGCCACCTGTGCTCTAGCCAGTGGCCTGTCCTTCGGCTCAGGGTTTTGCCACTGGCTGCTGCATT GTCCTGCACGCCACAGCTTGTATAAGTGCACCTGACACACCAGCGTCATCACAGAACTGCACAGAACAGA TGGGAATTCTCTGTTTAATTTCCCATCTTATCTAGCACATACTTTGCTGAATATAATTACCGTAGGCAGT AGAAGGCCACTATAGTTAGGGAAATGGGTACAGTATGTAGGGGACCCTAAAAGTAATGCACGAGAGTAGT TACCAAGGACTCGGGGTGCGGGGGGGGAGGGATTCTTCTGGTCAAATATCACTGGTCCTGAGTGGTTGGT GGCAGAGCCTCAATCCCAGCTCTCAGGTGGCAGAGGTAGGTGGATCTCTGTGAGTACAGCCTGATCTACA GATGGAGTTCCAGGACCGCCAGGGCTACACAAATAAACCCTGTATTGAAGAAGAAGAAAAATTCCACTGG TCCTGAAACAAAACCATGGATATCATTGTGTTCATACAGAGAGCAAGTCTGGGTGGGCCCAAGGGTTTCA GCTGCATTTGTATGTATGGATCTAAACAAATGTATATTTTTCAAGATGATTTTGCAGAAGTTATATTTTA AATGTTGATCAATACATTTATATTCATTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30314995 using vector (pDNR-LIB) specific primers. Forward Primer - name:M13 forward (-21), sequence:TGTAAAACGACGGCCAGT; Reverse Primer - name:T3-pDNR-LIB rv, sequence:CAAATTAACCCTCACTAAAGGGTGTTCACTTACCTACTGGGC. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30314995 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13419 same embryo
	EMAGE:13420 same embryo
	EMAGE:13417 same embryo
	EMAGE:13421 same embryo
	EMAGE:13413 same embryo
	EMAGE:13414 same embryo
	EMAGE:13415 same embryo
	EMAGE:13416 same embryo
	EurExpress:euxassay_017543 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source