EMAGE mouse gene expression spatial database

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EMAGE:13424

Fchsd1 FCH and double SH3 domains 1 ( MGI:2441771)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_017448. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_017448_01	euxassay_017448_02	euxassay_017448_03	euxassay_017448_04

euxassay_017448_05	euxassay_017448_06	euxassay_017448_07	euxassay_017448_08	euxassay_017448_09

euxassay_017448_10	euxassay_017448_11	euxassay_017448_12	euxassay_017448_13	euxassay_017448_14

euxassay_017448_15	euxassay_017448_16	euxassay_017448_17	euxassay_017448_18	euxassay_017448_19

euxassay_017448_20	euxassay_017448_21	euxassay_017448_22	euxassay_017448_23	euxassay_017448_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13424_wholemount_strong.wlz

13424_wholemount_moderate.wlz

13424_wholemount_weak.wlz

13424_wholemount_possible.wlz

13424_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13424_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9174
Entity Detected:	Fchsd1, FCH and double SH3 domains 1 ( MGI:2441771)
Sequence:	sense strand is shown >T9174 CCGCGATGCAGCCGCCGCCCCGAAAAGTGAAGCCAGCCCAGGAGGTTAAACTTCGCTTTTTGGAGCAGCT GAGCATCCTTCAGACCCGGCAGCAGAGGGAGGCGGATCTACTGGAGGACATCAGATCTTACAGCAAGCAA AGGGCAGCCATTGAACGGGAGTATGGCCAGGCCCTCCAGAAGCTGGCTGGGCCATTCCTGAAGAGAGAAG GGCAGCGGAGTGGAGAGGCAGACAGCAGGACAGTGTTTGGTGCCTGGCGCTGCCTTCTGGATGCCACTGT GGCTGGAGGCCAAACCAGACTCCAGGCGTCTGACCGATATCGCGACCTAGCAGGGGGTACTGGGAGAAGC GCCAAGGAGCAGGTGCTTAGAAAGGGGACAGAGAGCCTCCAGCAGGCACAGGCTGAGGTGTTACAGTCTG TCCGGGAGCTGAGCCGAAGTCGGAAGCTCTATGGGCAGCGACAGCGTGTATGGGCCTTGGCCCAGGAGAA GGCAGCTGATGTCCAGGCCAGGCTGAACCGAAGTGACCATGGGATCTTCCATTCTCGGACCAGCCTCCAG AAACTGAGCACCAAGCTGTCTGCCCAGTCAGCCCAGTATTCCCAGCAGCTGAGAGCAGCCCGCAATGAGT ACCTGCTAAATTTGGTGGCCACCAATGCTCACCTTGCCCACTACTACCAAGAGGAATTGCCAGCACTGCT CAAGGTCCTGGTAAGTGAGCTGTCAGAATACTTGAGGGACCCCCTGACTTTACTGGGACACACAGAGCTG GAAGCTGCGGAGATGATCCTGGAGCATGCCCGCCATGGGGGGAAGGCAACGTCTCAGGTAAACTGGGAAC AAGATGTGAAGCTGTTTCTTCAGGGGCCTGGAGTCTTTTCTCCCACCCCACCTCAGCAGTTCCAGCCGGC AGGGGCTGATCAGGTATGTGGCCTGGAGTGGGGAGCAGGAGGCATGGCTGGAGAGAGTGGCCTGGAGAAA GAGGTTCAGCGCTGGACAAGCAGGGCTGCCCGGGACTACAAGATCCAGCATCACGGGCATCGGGTCCTGC AGCGACTGGAGCAGAGGCGTCAGCAGGCCCCGGGGCGAGAAGCTCCAGGCGTAGAACAGCGGCTACAGGA AGTGAGGGAGAACATCCGACGGGCACAGGTGAGCCAGGTGAAAGGGGCCGCCCGGCTGGCCCTGTTACAG GAGGCTGGTCTCGATGTACAGCGCTGGCTGAAGCCAGCCATGACCCAAGCCCAGGATGAGGTGGAACAGG AGCGGCGACTTAGTGAAGCTCGACTGTCCCAGAGGGACCTCTCTCCCACGGCTGAGGATGCTGAGCTTTC TGACTTCGATGAATGTGAGGAAGCTGGGGAGCTCTTTGAGGAGCCTGCCCCACCAGCCCTGGCTACCAGA CCCCTCCCCTGCCCTGCACATGTAGTGTTCGGCTATCAGGCAGGACGTGAGGATGAGCTGACCATCACAG AGGGTGAGTGGCTGGAGGTCATAGAGGAAGGAGATGCTGACGAGTGGGTTAAGGCTCGGAACCAGCACGG TGAAGCGGGCTTTGTCCCTGAGCGATATCTCAACTTCCCGGATCTCTCCCTGCCTGAGAGCTGCCATGGC ATCGACAATCCCTCAGGAGGAGAGCCCACAGCATTCTTGGCACGCGCTCTGTACAGCTACACGGGACAGA GTGAAGAGGAGCTAAGTTTCCCTGAAGGGGCACTCATCCGCCTGTTGCCCAGGGCTCAAGATGGAGTGGA TGATGGTTTCTGGAGGGGAGAATTTGGGGGCCATGTTGGAGTCTTCCCATCTCTCCTGGTAGAGGAACTG CTTGGTCCCCCGGGGCCACCTGAGCTCTCTGACCCTGAACAGATGCTGCCGTCCCCTTCTCCTCCCAGCT TCTCCCCTCCTGCACCCACCTGTGCCTTGGACGGATCTACTGCGCCTGCTCTGCCTTCTGACAAAGTCCT TGACTGCCCTGGACCCCTAGACATGATGGTGCCTCGACTTAGGCCGATGCGTCCACCACCTCCCCCACCA GCCAAAGCCCCGGACCCTGGTCATCCCGATCCTCTCACTTGAAGGCCAGGAGTCATTGCCCGCCAGTGAT GCTGCTGTCCCTGTCTCCACGCTGACAGAAACCACCCCTTCAATGGTCTGGAGTGACATAGGCAAAAGCT GCAATCTTTCTCGATGTCCGTTCTCACCTCCATGGGTGGAGACTTACCCTGTTCCCATTTCTGGGATTTG GACCCATGCATCACCCCCTCCCATTGTTCTCATGTCATTTCTGGAAGTACTCTTTTGACTTCTTGCCATC ACCCCATATCTAAGGTGGGCTGGAACCCATCTCCCAAAGCCTTGCACGGCTCTGGCTCATCTCCGTCTTC CCCCTGCCTCTGTGGTCAACCCCACCCTGAATGCCAGGGTCACTAGGCCTGGGGCCAGGATAGGAGCAGA CCTTGGAAGTCAGAAGGAGCTGGAGCCAGGATGCAGAGCAACTGTAATGGTCTGAGCGGATTTATTGACA GTGAATAAAGGGTATGAAGTCCAAGCCAGGGCCTGGGCCTCTTGTGCCAAGATGGCAGGGGGCCTAAGGT GCTATTGCTTTAGGGGCCCACCGAGGGTAGGGCCTGGTCCCAGCAGCCACGCTCTAGCATGTGGAGCGAG GTTTTGGGGAAGGCAGGCCAGGCAACCTGTTGGCAGGTGGGGAAAGGGAACAGAATGAGGGGCTGGGAAT TGGGAGGGGCTCCTCCTGAGGGCTCTCAGCCGGGGACCATGTATCTCCAGATGGAAGTAGAGTTGGTCCC TCAACTGAGGGGCAGAGCTGTCCAGTGGAGGGGAGAGCCTTCCTGGCTATCCGTCCCCTGGCAATGCCTG CTGGTAACCCATCAGCACAGTGAAGACTGAGGCTTGGAAGAAAGGAAGGGAAGCAGTGTTGTTGCCTAGT CAAGCTCTGTTCTCCAGGCATTGTATTGTATCTCCTGGAGGAACCTGTGCCCGCTCCCCACACACTAAGC TTACAGTTCACCCACCTCCAGGATTCAGGGAGTTGATGGGCTTGAGTAGTTTAGATAGAGGGCAGGAGGG TGAGATTACCTGATCTGGAGCTCAGGGAGACTTGGCTCACATACCCCCTGCTTCTGGTGGTAGAGACTCC TGAGAGGAGGGGGAAGGATTCAGTGGGAACAGGAAGTGGATCATACCCACGTTGCCCAGAGTAGGGATGG TAAGATGTGTGTCCGCCACTGCCCAGTGCAGCCCTGGGCATGTCTTCATACCTGCTGACCTGAGGTGTCC AGTTTGGTTGGCTGTCCATTTGCTTCTTGACTAGACAGCCCTGGGCTTGGGTCTCTCCCTCTAGGGCCCA AGTTCAGTTCTGCAGAGGTTCCAGGGGTCCCCTCAAGTGTACAAGGGATGAGGCTGGCATCCTGGAGTCT TCCGTTCTGCACTGAGTGGGTGGCTGCGGCCTGGGAGGGTGGCGGCTCAGGGAGCAGCCTCTCAATGGCA GCTGTCCCTGTCCTAGGCTGCCCTCCTCCAGCCGCCTGACTACCCCCTGGCTCCTGCCCTCCACCAGAGC CCCAGCTTCTGTCCGTAGGGGAACCATCACGATGTTCATGTAGGCCGTAGCGCTTCTCAATGTGTGTTAC TCGGAATCTGGGTGGGAAGAGAATGTTGCAACTCAGGCTTACAGCTCAGAGGCTCCTAAACGTCCCTCCC TGGGTTTGACCTTGGGTAGGGAAGCCTTCTGAGACTGTGGGATACTGTGCCACCTGACCCCTGCTGTAGT TGCCTAGAGGAAATTCAGAGGAAAGAATTGGGGCCCAACTTTTGGGGGGTCAGGATTATTGGGGCTCCAG TTTAACCTCCCTTTTCTTGGACTACTATATCCCCACCTGCCCACTGAAAAAACAGTGGTCTCTCCAGGTC TGGGACGGCTTTTTCCTTCCTTAGAGCGACCCTGACGGACAAGTGGGGGCCTCTTGCTGCGGCTGCAGTG GATGCAAGGAGCTGCAGAGCCAAGGTGCACTGTGTGATGATGGGAGGGGGCTCCGTCCTGCAGGCTGGAG GTGGCATCCACACTGGACCACAGAAGGGAAGGGAGCAGAAACGATATCTTCAATTCCCTTCCTGCTCTTT CGTCCTGTTCTTTCAATATGCTCTTCAGACTTCCTCAAACCCCACCTTCCCCAGGTCTCAACATCCCTTT GCCTGTAATAAATTCACTGTTAAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6827858 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6827858 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13425 same embryo
	EMAGE:13423 same embryo
	EMAGE:13427 same embryo
	EMAGE:13422 same embryo
	EMAGE:13426 same embryo
	EurExpress:euxassay_017448 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source