EMAGE mouse gene expression spatial database

EMAGE:13452

Tln2 talin 2 ( MGI:1917799)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_017631. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_017631_01	euxassay_017631_02	euxassay_017631_03	euxassay_017631_04

euxassay_017631_05	euxassay_017631_06	euxassay_017631_07	euxassay_017631_08	euxassay_017631_09

euxassay_017631_10	euxassay_017631_11	euxassay_017631_12	euxassay_017631_13	euxassay_017631_14

euxassay_017631_15	euxassay_017631_16	euxassay_017631_17	euxassay_017631_18	euxassay_017631_19

euxassay_017631_20	euxassay_017631_21	euxassay_017631_22	euxassay_017631_23	euxassay_017631_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

wholemount mapping

Download individual expression domains:

Download all expression domains:

Find spatially similar wholemount expression patterns:

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T40218
Entity Detected:	Tln2, talin 2 ( MGI:1917799)
Sequence:	sense strand is shown >T40218 CCACAGACAGCTACACCAAGAGGGAGCTGATCGAGTGTGCCCGCTCTGTCACTGAGAAGCTAACAAAGAC CCCCATTGAACCCACACTTCTGTCGGTCTTTCCTTCTGCCCCTCTCTATAAGCCCCTGGCTTTGCTTCTG TATCGGAGTCCTTCCGCGGCAGCTGTTGCGGCTGGGGCTGCGGCTGCGAGTAGCACGGACTGGAGCATCC TCGCTCTGCAGTTCCTCTTCACGTCTGTGTCCTTGGTGCTGTCTGCTCTCCAGGCTGGAAACAAGGGGAC CCAGGCATGCATCACGGCCGCAACTGCAGTTTCTGGGATCATTGCCGATCTGGACACCACCATTATGTTT GCAACAGCTGGCACACTGAATGCAGAGAACGGCGAGACCTTTGCCGATCACAGGGAGAACATTCTGAAGA CAGCCAAGGCCTTGGTGGAAGACACAAAGCTCCTCGTGTC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 109307. Forward Primer - name:109307_F_cDNA_LOC434411, sequence:CCACAGACAGCTACACCAAGAG; Reverse Primer - name:109307_N_SP6_cDNA_LOC434411, sequence:GACACGAGGAGCTTTGTGTCTT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13451 same embryo
	EMAGE:13454 same embryo
	EMAGE:13455 same embryo
	EMAGE:13453 same embryo
	EurExpress:euxassay_017631 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source