EMAGE mouse gene expression spatial database

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EMAGE:13506

Med18 mediator of RNA polymerase II transcription, subunit 18 homolog (yeast) ( MGI:1914469)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_017581. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_017581_01	euxassay_017581_02	euxassay_017581_03	euxassay_017581_04

euxassay_017581_05	euxassay_017581_06	euxassay_017581_07	euxassay_017581_08	euxassay_017581_09

euxassay_017581_10	euxassay_017581_11	euxassay_017581_12	euxassay_017581_13	euxassay_017581_14

euxassay_017581_15	euxassay_017581_16	euxassay_017581_17	euxassay_017581_18	euxassay_017581_19

euxassay_017581_20	euxassay_017581_21	euxassay_017581_22	euxassay_017581_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13506_wholemount_strong.wlz

13506_wholemount_moderate.wlz

13506_wholemount_weak.wlz

13506_wholemount_possible.wlz

13506_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13506_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8136
Entity Detected:	Med18, mediator of RNA polymerase II transcription, subunit 18 homolog (yeast) ( MGI:1914469)
Sequence:	sense strand is shown >T8136 TGCGGGTGTGCACGCGTGGATTTAATTCCAGCTTTACAAGGATCTGTAGCGCGGACGGAGACGTGTTGCT AGTGTGGATCCTGTGCTTCTCCGGTTGGAAGATCTGTTTGCAGTTCTGTTGTCTTGAACCTAATTAGAGG CATCATGGAGGCGCCTCCAGTCACCATGATGCCCGTCACTGGGGGCACCATTAACATGATGGAGTACCTC CTGCAGGGAAGTGTTTTGGACCACAGTTTGGAAAGCCTCATCCATCGCCTCCGAGGTCTGTGTGACAACA TGGAGCCTGAGACCTTCCTTGATCACGAGATGGTCTTCCTGCTTAAGGGCCAGCAGGCCAGCCCGTTCGT CCTAAGGGCCCGGCGCTCCATGGACAGGGCAGGGGCACCCTGGCATCTGCGATACCTGGGACAGCCCGAA ATGGGGGACAAGAACCGCCACGCCCTGGTGCGCAACTGTGTGGACATCGCCACGTCTGAGAACCTCACGG ACTTCCTGATGGAGATGGGCTTCCGCATGGACCATGAGTTTGTCGCCAAAGGACACCTGTTTCGGAAGGG CATCATGAAGGTTGTGGTGTACAAGATTTTCCGAATCCTGGTCCCAGGGAACACGGACAGCACCGAGGCC CTGTCCCTCTCCTATCTTGTGGAATTAAGTGTTGTCGCCCCGGCTGGGCAGGACATGGTCTCTGATGACA TGAGGAACTTTGCAGAGCAGCTCAAACCTCTGGTTCACCTGGAAAAAATAGACCCCAAAAGGCTGATGTG ATGAAGAAAGTTTGTCGGCCTTTTAGGCCTGTCCCCACCTGGGAGGTGAGAGACTTCGAAAGTAGCCCAG GTACTGCTGCCAGCCGGGCCCACTTCCTTCCAGAAGATGATGGCGAGACCTGGTTCCTTTCAACTGTTTC CTTCACTTGGTAATGTCTCTTTTTTCTTGCAACAGGGTTTTACCATGGAATTCACTACATAAACTAGCTA CCCTGGAACTCAGGACCCTGTACTTCCCGCCTAAGTGCCTGCAAGGTCCTTATTAAACCTGACTGTTCAT CTTCTTGATAGGCTTTTCCAATGAAAGACCCAGGATAATATGGAGATTTGTTCAAGTCCTCCCTCCACTC AAAAAGAGGGAACTGGAATAAATTAGCTTTTCAGAGGAAAGGTCAAAAAACAACTCATAATATTTTGCTT TTTTTTCTTTTTTCTCTTCTTTTCTTTTCTTTTTTGTTTTTTTTTTTTTGTTTTTTGTTTGTTTGTTTGT TTGTTTGTTTGTTTTGTTTTTCAATACAAGACAGGGTTTCTCTGTGTTGCTTTGGCTGCCCTGGAACTCA CTTTGTAGACCAGGCTGGCCTCAAACTCAGAAATCCACCTGCCTCTGCCTCCCGAGTACTGGAATTAAAG GCGTGCGATAACACGCAAGCCCGGCTGTTTGTTTTTTTTTTGTTTTTTTTTTGTTTTTTTTAGAGAAACA TTTTTTATTCCTTTATTTATTTGTTGTTTTCTTCATATGTTTTAAATTGTGTTGTTTTGCCATTGATATA CAAGGTTTACAATGGTGGTGTGAGCCAGCATTGTGTGGTAATAAAGACTGAAAAGACAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30043405 using vector (pSPORT1) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30043405 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13507 same embryo
	EMAGE:13505 same embryo
	EMAGE:13504 same embryo
	EMAGE:13503 same embryo
	EMAGE:13502 same embryo
	EurExpress:euxassay_017581 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source