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EMAGE:13554

Sars2 seryl-aminoacyl-tRNA synthetase 2 ( MGI:1919234)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:13554 EMAGE:13554 EMAGE:13554 EMAGE:13554 EMAGE:13554
"Pseudo-wholemount" of euxassay_017843. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_017843_01 euxassay_017843_02 euxassay_017843_03 euxassay_017843_04
EMAGE:13554 EMAGE:13554 EMAGE:13554 EMAGE:13554 EMAGE:13554
euxassay_017843_05 euxassay_017843_06 euxassay_017843_07 euxassay_017843_08 euxassay_017843_09
EMAGE:13554 EMAGE:13554 EMAGE:13554 EMAGE:13554 EMAGE:13554
euxassay_017843_10 euxassay_017843_11 euxassay_017843_12 euxassay_017843_13 euxassay_017843_14
EMAGE:13554 EMAGE:13554 EMAGE:13554 EMAGE:13554 EMAGE:13554
euxassay_017843_15 euxassay_017843_16 euxassay_017843_17 euxassay_017843_18 euxassay_017843_19
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euxassay_017843_20 euxassay_017843_21 euxassay_017843_22 euxassay_017843_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:13554Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
13554_wholemount_strong.wlz
13554_wholemount_moderate.wlz
13554_wholemount_weak.wlz
13554_wholemount_possible.wlz
13554_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:13554_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T479
Entity Detected:Sars2, seryl-aminoacyl-tRNA synthetase 2 ( MGI:1919234)
Sequence:sense strand is shown

>T479
TCTCAGNCTGTTGGCCTACTGGGTTTCCACTCCAAGATGGCTGCGTCCATGGCGCGGCTTTGGTGGCCTT
TCCTGGCTCGCCAGGGGCTCCGGTCCCGAGGACGCTGCGTGTGCAGCCAGAACCCAAGGAGGAGTTTCGC
TACGGAGAAACGAATCCGGAACCTCCTGTACGAACACGCACGAGAGGGCTACAGCGAGCTTCCCTACTTG
GACATGGAGTCGGTGTGCGCATGCCCAGAAAAAGCCGCGCGCTCCCTGGAGCTCCGCAAGGGGGAGCTGC
GGCCGGCCGACCTGCCTGCGATTATCTCCACGTGGCAGGAGTTGAGGCAGCTTCGAGAGCAGATCCGGAG
CCTGGAGGCAGAGAAGGAGGCTGTGGCAGAGGCAGTGCGGGCCCTGCTGGCAAACCAAGACAGTGACCAA
GTGCAGAAGGACCCCCAATATCAGGGCCTGCGGGCCCGAGGCCGGGAGATCCGGAAGCAGCTCACACCCT
TGTACCCCCAAGAGACCCAGCTAGAGGAGCAGCTGTACCAGCAGGCACTGAGG
Notes:The probe template was PCR amplified from IMAGE:1480683 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1480683 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:13552 same embryo
 EMAGE:13555 same embryo
 EMAGE:13553 same embryo
 EMAGE:13550 same embryo
 EMAGE:13551 same embryo
 EurExpress:euxassay_017843 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS