Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:13607

Irgq immunity-related GTPase family, Q ( MGI:2667176)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:13607 EMAGE:13607 EMAGE:13607 EMAGE:13607 EMAGE:13607
"Pseudo-wholemount" of euxassay_017837. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_017837_01 euxassay_017837_02 euxassay_017837_03 euxassay_017837_04
EMAGE:13607 EMAGE:13607 EMAGE:13607 EMAGE:13607 EMAGE:13607
euxassay_017837_05 euxassay_017837_06 euxassay_017837_07 euxassay_017837_08 euxassay_017837_09
EMAGE:13607 EMAGE:13607 EMAGE:13607 EMAGE:13607 EMAGE:13607
euxassay_017837_10 euxassay_017837_11 euxassay_017837_12 euxassay_017837_13 euxassay_017837_14
EMAGE:13607 EMAGE:13607 EMAGE:13607 EMAGE:13607 EMAGE:13607
euxassay_017837_15 euxassay_017837_16 euxassay_017837_17 euxassay_017837_18 euxassay_017837_19
EMAGE:13607 EMAGE:13607 EMAGE:13607 EMAGE:13607 EMAGE:13607
euxassay_017837_20 euxassay_017837_21 euxassay_017837_22 euxassay_017837_23 euxassay_017837_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:13607Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
13607_wholemount_strong.wlz
13607_wholemount_moderate.wlz
13607_wholemount_weak.wlz
13607_wholemount_possible.wlz
13607_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:13607_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T63258
Entity Detected:Irgq, immunity-related GTPase family, Q ( MGI:2667176)
Sequence:sense strand is shown

>T63258
GTCTCACTAAGTTGCTTGGGCTGTCCTTGAACTCACTTTATTCCAGACAGGCCTTGGAATTTGGGATCCT
AACCCCTCAGCCTCCCAAGTAACTGGGATCACAGGCCTGCCTCACCTCCTGGCTTAGTAGTTTTCCCTGA
ACCTGTGATGTTCCAGTGGGTCAAGTACAAATAGAATCTGAGATGGATTTCCCCCCCCCCTCGGTGGCTT
TATCCACATACTTGGATCATAGTTCAACAATACAGTCTATTGTGAAAAATCATAACAGCATGAAAGTGAG
ACATTGCAACCACATTTGGGAAGCTGAGAAATCTTTGTTTCACCCCAGGACCACAGCGCTATGGAATAGT
GTTTCAAACAGTAGGTTTCCTCGCCTCATTTAACATAGTCTAGGCACTCCTCCACAGCCATGTCCAGGGT
GAACGTAAATTCTGTCAGTTAATATTAACCATCACAGCAGGCATCTAAGAACTTGCTCTGTTACATGTGC
ACATGTGTGGCTAAGCATGCACACACTGGCATGTGAATGTGCTCACACACATACCCAGCTCCCAGGTACT
GCAGCCCCCTCTGAGAAGCTTGACACTCAAATGCTACTCCTCTACTGCCTGGGCC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 72397. Forward Primer - name:072397_F_cDNA_AF322649, sequence:GTCTCACTAAGTTGCTTGGGCT; Reverse Primer - name:072397_N_SP6_cDNA_AF322649, sequence:GGCCCAGGCAGTAGAGGAGTA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:13606 same embryo
 EMAGE:13605 same embryo
 EMAGE:13609 same embryo
 EMAGE:13608 same embryo
 EMAGE:13604 same embryo
 EurExpress:euxassay_017837 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS