EMAGE mouse gene expression spatial database

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EMAGE:13717

Nr5a2 nuclear receptor subfamily 5, group A, member 2 ( MGI:1346834)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_019546. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_019546_01	euxassay_019546_02	euxassay_019546_03	euxassay_019546_04

euxassay_019546_05	euxassay_019546_06	euxassay_019546_07	euxassay_019546_08	euxassay_019546_09

euxassay_019546_10	euxassay_019546_11	euxassay_019546_12	euxassay_019546_13	euxassay_019546_14

euxassay_019546_15	euxassay_019546_16	euxassay_019546_17	euxassay_019546_18	euxassay_019546_19

euxassay_019546_20	euxassay_019546_21	euxassay_019546_22	euxassay_019546_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13717_wholemount_strong.wlz

13717_wholemount_moderate.wlz

13717_wholemount_weak.wlz

13717_wholemount_possible.wlz

13717_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13717_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

adrenal gland

weak

regional

weak expression: see section 10 11 12 18 19 20

pancreas

strong

regional

strong expression: see section 09 10 11 12 13 14 15 16 17 18 19

medulla oblongata basal plate mantle layer

moderate

regional

moderate expression: see section 12 13 weak expression: see section 14 15

midbrain mantle layer

moderate

regional

moderate expression: see section 09 10 12 13 14 17 18 weak expression: see section 08 16

ventral grey horn

strong

single cell

strong expression: see section 12 13 14 16 17 weak expression: see section 15

pharyngo-tympanic tube

strong

regional

strong expression: see section 04 05 moderate expression: see section 02 06 22 weak expression: see section 03 07

midgut

moderate

regional

moderate expression: see section 04 05 12 13 14 16 weak expression: see section 06 08 09 10 11 15 17 18 19 20 21

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T55133
Entity Detected:	Nr5a2, nuclear receptor subfamily 5, group A, member 2 ( MGI:1346834)
Sequence:	sense strand is shown >T55133 CTCTGAACTGCTCCAAGCAATGCTAATTATAAACTTGGTTTAAAGACACTGAATTTTAAAAGCATAATAA TTAAATACCTAATAGCAAATAAATGATATATCAGGGTATTTGTACTGCAAACTGTGAATCAAAGGCTGTA TGAATCAAAGGATTCATATGAAAGACATTGTAATGGGGTGGATTGAACTTACAGATGGAGACCAATACCA CAGCAGAATAAAAATGGACAGAACAATCCTTGTATATTTAAACTAATCTGCTATTAAGAAATTCAGAAGT TGATCTCTGTTATTAATTGGATTTGTCCTGAATTACTCCGTGGTGACGCTGAACAACTCAAGAATACATG GGCTGTGCTTGGCAGCCCCTCCCCATCCCTCCCACCACCACCACCCCCACCCCCACAAGGCCCTATACCT TCTGACCTGTGAGCCCTGAAGCTATTTTAAGGACTTCTGTTCAGCCATACCCAGTAGTAGCTCCACTAAA CCATGATTTCTGGATGTCTGTGTCTTAGACCTGCCAACAGCTAATAAGAACAATGTATAAATATGTCAGC TTGCATTTTAAATATGTGCTGAAGTTTGTTTTGTCGTGTGTTCGTAATTAAAAAGAAAACGGGCAGTAAC CCTCTTCTATATAAGCATTAGTTAATATTAAGGGAAATCAAACAAATCTAAGCCAATACTCCCAACAAGC AAGTTAGATCTTACTTCTGCTGCTGTTGCTGAAATGTGGCTTTGGCATGGTTGGGTTTCATAAAACTTTT TGGCCAAGAGGCTTGTTAGTATACATCCATCTGTTTAGTCATCAAGGTTTGTAGTTCACTTAAAAAAAAA TAAACCACTAGACATCTTTTGCTGAATGTCAAATAGTCACAGTCTAAGTAGCCAAAAAGTCAAAGCGTGT TAAACATTGCCAAATGAAGGAAAGGGTGAGCTGCAAAGGGGATGGTTCGAGGTTCATTCC
Notes:	The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:CTCTGAACTGCTCCAAGCAA; Reverse Primer - name:unspecified, sequence:GGAATGAACCTCGAACCATC. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13718 same embryo
	EMAGE:13720 same embryo
	EMAGE:13719 same embryo
	EMAGE:13716 same embryo
	EurExpress:euxassay_019546 same experiment
	MGI:4826787 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source