EMAGE mouse gene expression spatial database

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EMAGE:13762

Foxd2 forkhead box D2 ( MGI:1347471)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_019656. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_019656_01	euxassay_019656_02	euxassay_019656_03	euxassay_019656_04

euxassay_019656_05	euxassay_019656_06	euxassay_019656_07	euxassay_019656_08	euxassay_019656_09

euxassay_019656_10	euxassay_019656_11	euxassay_019656_12	euxassay_019656_13	euxassay_019656_14

euxassay_019656_15	euxassay_019656_16	euxassay_019656_17	euxassay_019656_18	euxassay_019656_19

euxassay_019656_20	euxassay_019656_21	euxassay_019656_22	euxassay_019656_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13762_wholemount_strong.wlz

13762_wholemount_moderate.wlz

13762_wholemount_weak.wlz

13762_wholemount_possible.wlz

13762_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13762_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

foot mesenchyme

moderate

regional

moderate expression: see section 19 20 21

physiological umbilical hernia

strong

regional

strong expression: see section 11

vibrissa

strong

regional

strong expression: see section 02 03 04 19 moderate expression: see section 05 18 20

hypothalamus mantle layer

moderate

regional

moderate expression: see section 08 09 10 11 13 weak expression: see section 14 15

diencephalon lateral wall mantle layer

strong

regional

strong expression: see section 10 11 12 13

naris

strong

regional

strong expression: see section 10 11 12 13 15

metanephros

strong

regional

strong expression: see section 07 09 10 11 17 18 19 moderate expression: see section 08

male reproductive system

moderate

regional

moderate expression: see section 11 weak expression: see section 14 15

genital tubercle of male

strong

regional

strong expression: see section 11 moderate expression: see section 10

tail mesenchyme

moderate

regional

moderate expression: see section 10 11 12 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T55050
Entity Detected:	Foxd2, forkhead box D2 ( MGI:1347471)
Sequence:	sense strand is shown >T55050 AAGTCTTGGCCATGCTGACTGCCCCGGCCCTGACTCCGGTGGCTGGCCACATCCGCCTCTCGCACCCAGG GGACTCACTCCTGTCTTCAGGGCCCTCGTTTGCCAGCAAAGTCGCGGGCCTCAGTGGCTGCCACTTTTGA CCGCACCGGGCTCGGGCCAGTTAGGCCCTCACTCCTTAACCTCTCTGGGGAGCTCGGCCGGTCCCAGCGG AATTCAGGGAGTCTATTTATGAAGAGTCTCCAGACCTAGGCCGTCGCGCGTTATCGGGCACTTCAAGCTG CAAGCTCAGAATGTCACCGGCAGTTGGGACGAAGCGGGCTCCCTGGCCTCCCTGATCTACGCAATAGGTT TAAAGCGGAGACCGCTTTTCTGTTTGAGCATCAAAAAGGAGAGCAGGAACCTTCTCTGTGTCTTGCCTTT GCGCCTTTCACAGTCAGAGAATGCTTTGGCAGAGATACGCCTGTCGGTTGTTGGATCGCGCCAGAATCAC CCATGGCTTGAAAAAACCCACTGCCCCATTCCTTCCTCAACTTTGAGACCCGCTCATCCAATATTATAAT TTAAAGACATCTGTTATCTGCTTTGTGCTTAAGAGAAATTTTCAAAAAAAATTTTTTTGGTTCCTTTTGC TGTTCTTCAAGGAAGCTGGTTCCCTCCAAGCCTAAGGCAGGCTCTTCACAGTCGGGAAGCTAAGAGAAGT GAAACGGGGTCTCCACAATCCTTGAAGAAACCGCAGAGCCCATTTCCCCGAGGAAAATGTTTTCTCTCTG GTGTTTGGCTGTTTTTTATTTTATTTTTTATTTTAAAAAAAGCCCTCTTTGGCTTTTCCCGTGGTCCATA GGCCCAGCCATCCAGGGCTAGCGGTCCTCTTGGCCTTCTCCTCTGCCCCAGGCCCTGTGATATTTATTGT AGTTAAAGGCAATGAGAGTGGGTGGGAGAAGAGGGTCTGGAGAGGTGGGAATT
Notes:	The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:AAGTCTTGGCCATGCTGACT; Reverse Primer - name:unspecified, sequence:AATTCCCACCTCTCCAGACC. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13761 same embryo
	EurExpress:euxassay_019656 same experiment
	MGI:4824901 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source