EMAGE mouse gene expression spatial database

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EMAGE:13799

Stat5a signal transducer and activator of transcription 5A ( MGI:103036)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_019566. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_019566_01	euxassay_019566_02	euxassay_019566_03	euxassay_019566_04

euxassay_019566_05	euxassay_019566_06	euxassay_019566_07	euxassay_019566_08	euxassay_019566_09

euxassay_019566_10	euxassay_019566_11	euxassay_019566_12	euxassay_019566_13	euxassay_019566_14

euxassay_019566_15	euxassay_019566_16	euxassay_019566_17	euxassay_019566_18	euxassay_019566_19

euxassay_019566_20	euxassay_019566_21	euxassay_019566_22	euxassay_019566_23	euxassay_019566_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13799_wholemount_strong.wlz

13799_wholemount_moderate.wlz

13799_wholemount_weak.wlz

13799_wholemount_possible.wlz

13799_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13799_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

strong

regional

strong expression: see section 17 moderate expression: see section 14 15 18 weak expression: see section 13 16

olfactory cortex ventricular layer

weak

regional

weak expression: see section 11 12 17 18

telencephalon ventricular layer

weak

regional

weak expression: see section 09 10 11 12 17 18 20

liver lobe

moderate

regional

moderate expression: see section 02 03 05 06 07 weak expression: see section 04 08 09 10 11 12 13 14 15 17 18 19 21 22 23 24

bladder

moderate

regional

moderate expression: see section 17 weak expression: see section 16

urethra of male

moderate

regional

moderate expression: see section 17 weak expression: see section 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T55192
Entity Detected:	Stat5a, signal transducer and activator of transcription 5A ( MGI:103036)
Sequence:	sense strand is shown >T55192 CACGTTTCACAGGGCTACCTTGCCCACACAGTTCTGGGTTTGTGGCTAAAGCGGTGGTGACCTTTTTGTT CAGACCTCAAGGGCCCCCAGGGCCTCTCGTGTAAGAGCTGAACCTATCATTGCTGACAAACCTATTTCTC CGGTGTCCTTTTTCTGTCCAATGGCCATTTCAGTGAAATTCTAGAAAAGGCAGGGAGGCAGGTTTAGGCA ACTAAGTTGGAGTTTTACTCCTAAGCTAGAAGCTTCGCCCAGACCGGTGTGCTCCTGTCCTCGCACAGGT GGAAGATTGGGGTTCATCTTAGTGACCCTTTATACCTTTGTGTATACATACGGGCTGCAGACTTTGTGAT TGCTCGGTGTGCTTAAGCTGTTCCCTTCAACACAGCAGAGGGCTGCCACAGCCGAGTGTCAGTTCTTGCG CCAGGGTGGATGGACGTGAGATTCAAGTCTAACGGCCTTGTCCACGTTCCCACCATCCCTTTTCTCCATT CGATATCCTCACCCTTCCAGATGGATTCATCCTTCTTGCTTTTTTTTTTTTTTTTATGTTTTTGCTTTGC TTTTTTGAGACAAGGTCTCTCCATATATCCCAGACTATCCATGAATGATCCTCCTACCTGTTTCTAGAGT GCTAGGATTACAGGCATGCATGACCACACGTGGCCTCATCCTTTCTTCCTTTCCTGTTTGCAACCTTGCT TATTATATCAGAAAGGAGGGGAATACTGGGGGTCTGGGAGGAGGAAACCTGGGGCGAAAAACCTGTAGCA CACAAAACCTGTACACACTGTCTGAAGGAAGGGGGTGGGGAGCGCTCAGTCTGCTCCACTCTGCTGGGCG GCAAGACCTAGAACGGGCCCTGCACTGTCCCACCATGGGTTGTGGTCTCAGAAATCGCTTCAGCAGCTCT TCCCTGGAGGCTGTGACAGAGCAGCCAGGAGGAGCCGACTAGGAGTCCTGGCTTCCAACCTCTCTGAAGG AAGCAGAGGGATG
Notes:	The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:CACGTTTCACAGGGCTACCT; Reverse Primer - name:unspecified, sequence:CATCCCTCTGCTTCCTTCAG. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13801 same embryo
	EMAGE:13798 same embryo
	EMAGE:13800 same embryo
	EurExpress:euxassay_019566 same experiment
	MGI:4828488 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source