EMAGE mouse gene expression spatial database

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EMAGE:13808

Hoxc6 homeobox C6 ( MGI:96197)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_019596. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_019596_01	euxassay_019596_02	euxassay_019596_03	euxassay_019596_04

euxassay_019596_05	euxassay_019596_06	euxassay_019596_07	euxassay_019596_08	euxassay_019596_09

euxassay_019596_10	euxassay_019596_11	euxassay_019596_12	euxassay_019596_13	euxassay_019596_14

euxassay_019596_15	euxassay_019596_16	euxassay_019596_17	euxassay_019596_18	euxassay_019596_19

euxassay_019596_20	euxassay_019596_21	euxassay_019596_22	euxassay_019596_23	euxassay_019596_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13808_wholemount_strong.wlz

13808_wholemount_moderate.wlz

13808_wholemount_weak.wlz

13808_wholemount_possible.wlz

13808_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13808_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

ventral grey horn

strong

regional

strong expression: see section 11 moderate expression: see section 12 13 14 15 16 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T55078
Entity Detected:	Hoxc6, homeobox C6 ( MGI:96197)
Sequence:	sense strand is shown >T55078 ACAGCCTGGGAGGAAAAGAGGAAAAGCGAGAAGAGACAGAAGAAGAGAAGCAGAAAGAGTGACCAGGACT GTCCTTGCACCCCTCTCTTCTCCCTTGCTCATCCCAGCTCCCCGAATCACACACTCTGTATTTATCTCTG GCACAATGATGTGTTGTGACTTCCTAAAACAAAACAGGGAGTCAGACGTGGACCTAAAAGTCAGCTTTGG ACCCCCTCCCTCACTGCACAACTCTTCACTCGTCTCTTCCTCTAGCTCCCTCGCTAGCTTCTTCCTGGCT TGTCTGCAGGCCCCTTCCCTCGCCTAGGCCTAGGAGACCTGAACCTCGCTTCAGACTCCTCAGATGTCTC TCCAAGTGAGCCCTAGGCTCCCTCCCCACCCCCGACGCCCTTCACCCCCGCCCTCCGGCGCAGAGAGCTG GCTCCAGGGCCTGGGGCCTCCACTCGGGGGCCTCAGGGCCCGGCCTGACAGCCGGAGGGCAGGAGGCCTC AGGAGGGCGCGCCTTGGCCCTGCAACAACCCCCAGGGCCTCTCCCCAGTCCCTGCCCGGTCCCTCTGCCC CAGCAAATGCCCAGTCCAGGCAAATTGTATTTAAAGATTCCTGGGGGTCATTATGGCATATTTCGAACTG TGACCGTTTCTGTGTGAATATTTCTAGCTGTATTTGTGGTCTCTGTATTTATATTTATGTTTAGCACCGT CAGTGTTCCTATCTCATTTAAAAAAGGATTTAAAAAAAAAAGAGGGAAAATAACAAAAAGAGAAAAAAAG TGAATGACGTTTGTTTAGCCAGTAGGAGAAAATAAATAAAGAAATAACCCCCTACGTGTTATCCTCCTGT ATAAATCCGACCTCTGGATCCGTTCTCGAAT
Notes:	The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:ACAGCCTGGGAGGAAAAGAG; Reverse Primer - name:unspecified, sequence:ATTCGAGAACGGATCCAGAG. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13809 same embryo
	EMAGE:13810 same embryo
	EurExpress:euxassay_019596 same experiment
	MGI:4825431 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source