EMAGE mouse gene expression spatial database

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EMAGE:13818

Dpf1 D4, zinc and double PHD fingers family 1 ( MGI:1352748)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_019654. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_019654_01	euxassay_019654_02	euxassay_019654_03	euxassay_019654_04

euxassay_019654_05	euxassay_019654_06	euxassay_019654_07	euxassay_019654_08	euxassay_019654_09

euxassay_019654_10	euxassay_019654_11	euxassay_019654_12	euxassay_019654_13	euxassay_019654_14

euxassay_019654_15	euxassay_019654_16	euxassay_019654_17	euxassay_019654_18	euxassay_019654_19

euxassay_019654_20	euxassay_019654_21	euxassay_019654_22	euxassay_019654_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13818_wholemount_strong.wlz

13818_wholemount_moderate.wlz

13818_wholemount_weak.wlz

13818_wholemount_possible.wlz

13818_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13818_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon lateral wall mantle layer

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 weak expression: see section 15 16 17 18 19

telencephalon mantle layer

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 19 20 21 22 23 weak expression: see section 16 17 18

olfactory cortex mantle layer

moderate

regional

moderate expression: see section 13 14 15 19 20 21 weak expression: see section 18

medulla oblongata

moderate

regional

moderate expression: see section 09 10 12 13 14 weak expression: see section 06 07 08 15 16 17

metencephalon

moderate

regional

moderate expression: see section 05 09 10 12 13 14 weak expression: see section 06 07 08 15 16 17 18

midbrain mantle layer

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 12 13 14 15 weak expression: see section 16 17 18

spinal cord mantle layer

weak

regional

weak expression: see section 08 09 10 13 14 15 16 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T55033
Entity Detected:	Dpf1, D4, zinc and double PHD fingers family 1 ( MGI:1352748)
Sequence:	sense strand is shown >T55033 AGGATCTTTGGGTGGTGCTATCTCCTGCCTCTTGGAGCTCCTGGCGCTCCCCACCCGGTGTCCCCAGTGG AAGGGATGGGGTGAAGCCCAGAGTGGGGGGGGGCAAGGTGTTCTCCCTCTGCAAGTGGAATGTTACCCTG TGGGTGGCTGGGTCCAACAGGGTCCCTCCTGTCCCCCCTCTTCATCCCTTGACAAATGGGCACCAGGCTT CTGCTCTCCTCAAAGCCATACCCCCGCCTTTGGGCGGGCATAGAGGGGTAGTGGATGCTAGCCAGCAGCA CGGAAAGAGCCTTTTTCTAAAGAAAAAGACAAAACGTGGAAAAAAAAGGGAAAAAAATTAATATATACAA AGAGTCCTATAAAGCCTGGCTGGGTGGAGAGGCACTGTTGAGTGTCTGCTGGGGACCTGACTTTACCAGT TTCCTGAATGGCGCCTCCCCACCTCATTTCTGGAGTTGCAATGGTCTCAACTCCCATCTGAGGTGGGTAC CACCCCTTCCTCAGTACCCACCGTGGATACTGCATGTGAACTATGGTTTTGAACTCTTCCTCCTCCTCCT TGAGAGCCCCGCCCTGCGCCCGCGTGGTGCCTGCCTGCCAGGCCTGGGGCGTGCAGCCGGGGAGGCGGGT GGGGTGAGGCAGGCAGGCAGCCAGCCCCCTGCAGTGAGAAGCACAGATTGCAATGGACTCAGTTTTTTTT TTTTTTTTTTTTTTTTTTCCTTTCTCCCTTCCCACCCCTTTCCTTCCCTACCCAGCCAGGCTGGGCTGCC TCCTGCCCCCCTCGCTAGCCATTTGGGGGTGGCAAGGGGGTGTGGTTGTTTCTCGTGGTTGTGTGTGTTT GTTGTTCGGGTTTTTAAAAGGGGAAATTGAGACTGCAAGTGGGGGAGGTGGAGGGTCTGGGGGAGTCTGC CCCCAATCCAGGAGTACCCCCCTTGCCACCAAGTCTCCTTTATTGCCTGCCTCTGCT
Notes:	The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:AGGATCTTTGGGTGGTGCTA; Reverse Primer - name:unspecified, sequence:AGCAGAGGCAGGCAATAAAG. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13819 same embryo
	EMAGE:13817 same embryo
	EurExpress:euxassay_019654 same experiment
	MGI:4824378 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source