Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:13849

Zbtb2 zinc finger and BTB domain containing 2 ( MGI:2685949)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:13849 EMAGE:13849 EMAGE:13849 EMAGE:13849 EMAGE:13849
"Pseudo-wholemount" of euxassay_019671. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_019671_01 euxassay_019671_02 euxassay_019671_03 euxassay_019671_04
EMAGE:13849 EMAGE:13849 EMAGE:13849 EMAGE:13849 EMAGE:13849
euxassay_019671_05 euxassay_019671_06 euxassay_019671_07 euxassay_019671_08 euxassay_019671_09
EMAGE:13849 EMAGE:13849 EMAGE:13849 EMAGE:13849 EMAGE:13849
euxassay_019671_10 euxassay_019671_11 euxassay_019671_12 euxassay_019671_13 euxassay_019671_14
EMAGE:13849 EMAGE:13849 EMAGE:13849 EMAGE:13849 EMAGE:13849
euxassay_019671_15 euxassay_019671_16 euxassay_019671_17 euxassay_019671_18 euxassay_019671_19
EMAGE:13849 EMAGE:13849 EMAGE:13849 EMAGE:13849 EMAGE:13849
euxassay_019671_20 euxassay_019671_21 euxassay_019671_22 euxassay_019671_23 euxassay_019671_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:13849Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
13849_wholemount_strong.wlz
13849_wholemount_moderate.wlz
13849_wholemount_weak.wlz
13849_wholemount_possible.wlz
13849_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:13849_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T55212
Entity Detected:Zbtb2, zinc finger and BTB domain containing 2 ( MGI:2685949)
Sequence:sense strand is shown

>T55212
TCAGAGATAAAGCGGGCACTGCTATGCAAATCTGGAAGGTGTGGGAGACTTTAAACCCGAGAGAGAGAAT
GTTTTTAATCTTTGTCCTCTAGGTGTCTGATTTCAGAATGTATCCTTTGAGATTATGTCCGGTTAAGGAA
AATCACTAGTTAAGAGAACTACTTTAACAAATGCAAAAGAGTAATAATGTGACGGCAATCTTCATTTTTG
GATAAAACCTGACGGGCTATGAATTTGTCTGCGAGCGAACGCATATGGATACTTGCTACAGGCAAGGGGA
TTGGTGCGCATGCCTTACCCCATGGCTGGCCTTTTTCCAAGAAGAACAAGAAGATGCTGTGTTTCGGTTC
CTGACACACTTGTTTTAAAATGAGATTTGAAAACGTGATCCTGTAGAATGTCTTTCTGAACAACAATGGC
CAATGGTGAGCTTGTGTGCTTGTGTGTGAGCCAGCTAGCCAACTGCCTGTAAATCAGTTGTGCGCCCTGC
AGTGACGCACCACACAAGGCAAGTTGTGAGCCAAAAGTATAACTTTAATGAGGGCTAAAGTTGATGCTGC
CTGGGCGCCAGGTGGGATGGGAGCAGGAGGATTAGGAGTTTGAGGCCAGCCTGGGCAAGACCTTGTCCAA
AAAAATAAAAAAATGCAATGCCCATAGTTCATTGGTGACATCTACTAAAGCATCAAAAATAACAAATCAG
GACACAAAATCTTTTTGGAGGAAAAATAAACACAATTGATTTTGTTGTATATCCTCCAGTTTTTGTTAAC
ACCTGAAATTTTCCCCAGTGGCCTAACAGCATGGAAAGCTGTCCATTAATCTTCCCTTGGGCCAATAAGA
AAGTGGTCTCCCAGGATG
Notes:The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:TCAGAGATAAAGCGGGCACT; Reverse Primer - name:unspecified, sequence:CATCCTGGGAGACCACTTTC. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:13850 same embryo
 EMAGE:13851 same embryo
 EMAGE:13848 same embryo
 EurExpress:euxassay_019671 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS