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EMAGE:13964

Stx19 syntaxin 19 ( MGI:1915409)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:13964 EMAGE:13964 EMAGE:13964 EMAGE:13964 EMAGE:13964
"Pseudo-wholemount" of euxassay_007866. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_007866_01 euxassay_007866_02 euxassay_007866_03 euxassay_007866_04
EMAGE:13964 EMAGE:13964 EMAGE:13964 EMAGE:13964 EMAGE:13964
euxassay_007866_05 euxassay_007866_06 euxassay_007866_07 euxassay_007866_08 euxassay_007866_09
EMAGE:13964 EMAGE:13964 EMAGE:13964 EMAGE:13964 EMAGE:13964
euxassay_007866_10 euxassay_007866_11 euxassay_007866_12 euxassay_007866_13 euxassay_007866_14
EMAGE:13964 EMAGE:13964 EMAGE:13964 EMAGE:13964 EMAGE:13964
euxassay_007866_15 euxassay_007866_16 euxassay_007866_17 euxassay_007866_18 euxassay_007866_19
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euxassay_007866_20 euxassay_007866_21 euxassay_007866_22 euxassay_007866_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:13964Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
13964_wholemount_strong.wlz
13964_wholemount_moderate.wlz
13964_wholemount_weak.wlz
13964_wholemount_possible.wlz
13964_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:13964_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T3086
Entity Detected:Stx19, syntaxin 19 ( MGI:1915409)
Sequence:sense strand is shown

>T3086
TGGCCTCGAGGCCAGATTCGGCACGAGGAGTCANAGGTTGAAAATGGTCCATCATCAGTGGTCACAAGGA
TACTGAAGTCTCAGTATGCTGCGATGTTCCGCCGCTTCCAGCAAACCATGTTTCTATACAATGACACAAT
AGCTTTAAAGCAAGAAAAATGCAAAACGTTTATTGTTCGTCAGCTTGAAGTGGCCGGAAAAGAAGTTTCT
GAAGAAGAAGTGAATGACATGCTTCACCATGGAAAATGGGAAGTTTTCAACGAAAGCTTACTTACAGAAA
CCAGTATCACTAAAGCACAGCTCTCGGAAATTGAACAGAGACACAAGGAACTTGTGAATTTGGAGAACCA
AGTGAAGGACCTCCGGGACCTTTTCATTCAGATATCTCTTCTGGTAGAGGAACAGGGAGAGAGCATCAAC
AGCATTGAGGTGATGGTGAACAGCACAAAAGATTATGTTAACAACACCAAAGAGAAATTTGGACTGGCTG
TAAAATACAAAAAAAGAAACCCCTGCCGGGCCCTGTGCTGTTGCTGTTGCCCACGCTGTGGCTCCAAATA
AAGATGCT
Notes:The probe template was PCR amplified from IMAGE:1546943 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1546943 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:13966 same embryo
 EMAGE:13963 same embryo
 EMAGE:13967 same embryo
 EMAGE:13962 same embryo
 EMAGE:13965 same embryo
 EurExpress:euxassay_007866 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS