EMAGE mouse gene expression spatial database

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EMAGE:14017

Slc22a13 solute carrier family 22 (organic cation transporter), member 13 ( MGI:2143107)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007886. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007886_01	euxassay_007886_02	euxassay_007886_03	euxassay_007886_04

euxassay_007886_05	euxassay_007886_06	euxassay_007886_07	euxassay_007886_08	euxassay_007886_09

euxassay_007886_10	euxassay_007886_11	euxassay_007886_12	euxassay_007886_13	euxassay_007886_14

euxassay_007886_15	euxassay_007886_16	euxassay_007886_17	euxassay_007886_18	euxassay_007886_19

euxassay_007886_20	euxassay_007886_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14017_wholemount_strong.wlz

14017_wholemount_moderate.wlz

14017_wholemount_weak.wlz

14017_wholemount_possible.wlz

14017_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14017_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

medulla oblongata basal plate mantle layer

moderate

regional

moderate expression: see section 13 14 weak expression: see section 06 07

facial vii ganglion

strong

regional

strong expression: see section 02 03 16

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 05 moderate expression: see section 15

trigeminal v ganglion

strong

regional

strong expression: see section 02 03 04 05 06 07 08 16 17 18 19

vagus x ganglion

strong

regional

strong expression: see section 06 moderate expression: see section 15

trigeminal v nerve

moderate

regional

moderate expression: see section 09

dorsal root ganglion

strong

regional

strong expression: see section 04 05 06 07 08 09 12 13 14 15

neural retina

moderate

regional

moderate expression: see section 02 03 04 weak expression: see section 21

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1372
Entity Detected:	Slc22a13, solute carrier family 22 (organic cation transporter), member 13 ( MGI:2143107)
Sequence:	sense strand is shown >T1372 TCCTCGAGNCTGTTGGCCTACTGGATACCTGTGTGGTGTTCCTAGACTCAGGAGTGAGAAGCAGACATGG CTCAATTTGCACAGGTCATGGCTGAAGTGGGCGACTTTGGTCGCTTTCAGGTGCGGTTGACCATCCTGAT GGGCATTCCCAATTTCCTGGCTGCATTCTTCATATTTGGCCAAGTCTTCATGGTCCTTGATGAGGCTCAC CACTGTTCAGTGTCCTGGGTTAAGAACCACACTTTCAACCTAAGTGCCGCTGAGCAGCTGGCTATAAGCA TCCCCAACGACACAGCGGGCAGACCCGAGTCCTGCCTCATGTTCCGGCCACCTCCTGACAGTGCCAGCCT GGAAGACATCCTGAGCCACCGCTTCAATGAGACACAGGCCTGTGACTCAGGCTGGGACTATCCTGAGAAC CGGCCTCAGTCCCTAAAGAAAGAGTTTGACCTGGTGTGTGATCGAAAGAACCTGAAGAAGACCTCCCAGT CGGTGTTCATGGCTGGTCTCCTTGTTGGGGCCCTGGTCTTTGGGCCTGTCTGTGACTGGATTGGCCGCAG ACCCTCCCTCCTGATGCAGGTGCTTCT
Notes:	The probe template was PCR amplified from IMAGE:2331778 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2331778 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14020 same embryo
	EMAGE:14018 same embryo
	EMAGE:14019 same embryo
	EurExpress:euxassay_007886 same experiment
	MGI:4828132 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source