EMAGE mouse gene expression spatial database

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EMAGE:14026

Ppp1r1a protein phosphatase 1, regulatory (inhibitor) subunit 1A ( MGI:1889595)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001697. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001697_01	euxassay_001697_02	euxassay_001697_03	euxassay_001697_04

euxassay_001697_05	euxassay_001697_06	euxassay_001697_07	euxassay_001697_08	euxassay_001697_09

euxassay_001697_10	euxassay_001697_11	euxassay_001697_12	euxassay_001697_13	euxassay_001697_14

euxassay_001697_15	euxassay_001697_16	euxassay_001697_17	euxassay_001697_18	euxassay_001697_19

euxassay_001697_20	euxassay_001697_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14026_wholemount_strong.wlz

14026_wholemount_moderate.wlz

14026_wholemount_weak.wlz

14026_wholemount_possible.wlz

14026_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14026_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon

weak

homogeneous

weak expression: see section 08 09 10 11 12 13 14 15 16 17 18 19

telencephalon mantle layer

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 19 20 weak expression: see section 21

olfactory cortex marginal layer

moderate

regional

moderate expression: see section 12 13 14 18 19

olfactory cortex ventricular layer

moderate

regional

moderate expression: see section 12 13 18 19

telencephalon ventricular layer

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 17 18 19 20 weak expression: see section 21

hindbrain

weak

homogeneous

weak expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19

midbrain

weak

homogeneous

weak expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 04 15

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 16 17 18 19 20 21

vagus x ganglion

moderate

regional

moderate expression: see section 05

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 06 07 moderate expression: see section 04 05 15 16 17

spinal cord

weak

homogeneous

weak expression: see section 03 04 05 06 07 08 09 10 11 12 13 14

dorsal root ganglion

moderate

regional

moderate expression: see section 02 03 04 09 10 11 12 13

neural retina

weak

regional

weak expression: see section 01 02 03 04 05 21

nasal cavity olfactory epithelium

weak

regional

weak expression: see section 09 10 11 12 13 15 16 17 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T3116
Entity Detected:	Ppp1r1a, protein phosphatase 1, regulatory (inhibitor) subunit 1A ( MGI:1889595)
Sequence:	sense strand is shown >T3116 TGGCCTCGAGGCCAGATTCGGACGAGGGAGGGAGCGCGCCCGCCCCAGCCCGAGGTCCGCCGCTGCCTGA CCGGGAGCCATGGAGCCCGACAACAGCCCACGGAAGATCCAGTTTACGGTCCCGCTGCTGGAGCCTCACC TGGACCCGGAGGCGGCGGAGCAGATTCGGAGGCGCCGCCCCACCCCTGCCACACTTGTGCTGACCAGTGA TCAGTCCTCCCCAGAAATAGATGAAGACCGGATCCCCAACTCACTTCTCAAGTCCACCTTGTCAATGTCT CCACGGCAACGGAAGAAGATGACAAGGACCACACCCACCATGAAAGAGCTCCAGACGATGGTTGAACATC ACCTAGGGCAACAGAAGCAAGGGGAAGAACCTGAGGGAGCCACTGAGAGCACAGGGAACCAGGAGTCCTG CCCACCTGGGATCCCAGACACAGGCTCGGCGTCAAGGCCAGATACCCCCGGGACAGCACAAAAGTCTGCA GAATCCAACCCCAAGACTCAGGAGCAGTGTGGTGTGGAGCCCAGAACAGAGGATTCTTCAG
Notes:	The probe template was PCR amplified from IMAGE:1547348 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1547348 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14028 same embryo
	EMAGE:14027 same embryo
	EMAGE:14025 same embryo
	EurExpress:euxassay_001697 same experiment
	MGI:4827372 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source