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EMAGE:14031

C1qtnf3 C1q and tumor necrosis factor related protein 3 ( MGI:1932136)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:14031 EMAGE:14031 EMAGE:14031 EMAGE:14031 EMAGE:14031
"Pseudo-wholemount" of euxassay_001714. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_001714_01 euxassay_001714_02 euxassay_001714_03 euxassay_001714_04
EMAGE:14031 EMAGE:14031 EMAGE:14031 EMAGE:14031 EMAGE:14031
euxassay_001714_05 euxassay_001714_06 euxassay_001714_07 euxassay_001714_08 euxassay_001714_09
EMAGE:14031 EMAGE:14031 EMAGE:14031 EMAGE:14031 EMAGE:14031
euxassay_001714_10 euxassay_001714_11 euxassay_001714_12 euxassay_001714_13 euxassay_001714_14
EMAGE:14031 EMAGE:14031 EMAGE:14031 EMAGE:14031 EMAGE:14031
euxassay_001714_15 euxassay_001714_16 euxassay_001714_17 euxassay_001714_18 euxassay_001714_19
EMAGE:14031 EMAGE:14031 EMAGE:14031 EMAGE:14031 EMAGE:14031
euxassay_001714_20 euxassay_001714_21 euxassay_001714_22 euxassay_001714_23 euxassay_001714_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:14031Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
14031_wholemount_strong.wlz
14031_wholemount_moderate.wlz
14031_wholemount_weak.wlz
14031_wholemount_possible.wlz
14031_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:14031_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
limb
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 09 22 23 24
vertebral axis musculature
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
telencephalon ventricular layer
weak weak
homogeneousweak expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21
lower jaw molar
strong strong
regionalstrong expression: see section 06 07 08 19 20 21
upper jaw molar
strong strong
regionalstrong expression: see section 06 07 08 18 19 20 21
nucleus pulposus
strong strong
regionalstrong expression: see section 12 13 14
tail
strong strong
regionalstrong expression: see section 13 14 15 16 17 18
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T1394
Entity Detected:C1qtnf3, C1q and tumor necrosis factor related protein 3 ( MGI:1932136)
Sequence:sense strand is shown

>T1394
CNCGGTACCTCCAGCCCCTGTCAAGCTTCCCTGCGAGACTCTTGTCGATTTGCCGATTTGCCGAGCCATG
CTCGGGAGGCAGCGCATCTGGTGGCACCTGCTGCCTTTGCTTTTCCTCCCATTTTGCCTGTGTCAAGATG
AATACATGGAGTCTCCACAAGCTGGAGGACTGCCCCCAGACTGCAGCAAGTGTTGCCATGGAGATTATGG
ATTTCGTGGTTACCAAGGGCCCCCTGGACCTCCAGGTCCTCCTGGCATTCCAGGAAACCATGGAAACAAT
GGGAACAATGGAGCTACTGGCCATGAAGGGGCCAAAGGTGAGAAAGGAGACAAAGGCGACCTAGGCCCTC
GAGGAGAACGGGGGCAGCATGGCCCCAAAGGAGAGAAAGGCTACCCAGGGGTGCCACCAGAACTGCAGAT
TGCATTCATGGCTTCTCTAGCAACTCACTTCAGCAATCAGAACAGTGGC
Notes:The probe template was PCR amplified from IMAGE:2372664 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2372664 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:14032 same embryo
 EMAGE:14029 same embryo
 EMAGE:14030 same embryo
 EurExpress:euxassay_001714 same experiment
 MGI:4823539 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS