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EMAGE:14040

Fam195a family with sequence similarity 195, member A ( MGI:1915491)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:14040 EMAGE:14040 EMAGE:14040 EMAGE:14040 EMAGE:14040
"Pseudo-wholemount" of euxassay_001688. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_001688_01 euxassay_001688_02 euxassay_001688_03 euxassay_001688_04
EMAGE:14040 EMAGE:14040 EMAGE:14040 EMAGE:14040 EMAGE:14040
euxassay_001688_05 euxassay_001688_06 euxassay_001688_07 euxassay_001688_08 euxassay_001688_09
EMAGE:14040 EMAGE:14040 EMAGE:14040 EMAGE:14040 EMAGE:14040
euxassay_001688_10 euxassay_001688_11 euxassay_001688_12 euxassay_001688_13 euxassay_001688_14
EMAGE:14040 EMAGE:14040 EMAGE:14040 EMAGE:14040 EMAGE:14040
euxassay_001688_15 euxassay_001688_16 euxassay_001688_17 euxassay_001688_18 euxassay_001688_19
EMAGE:14040 EMAGE:14040 EMAGE:14040
euxassay_001688_20 euxassay_001688_21 euxassay_001688_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:14040Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
14040_wholemount_strong.wlz
14040_wholemount_moderate.wlz
14040_wholemount_weak.wlz
14040_wholemount_possible.wlz
14040_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:14040_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
axial musculature
moderate moderate
regionalmoderate expression: see section 03 04 05 06 17 18 19 20
adrenal gland
strong strong
homogeneousstrong expression: see section 06 07 08 13 14
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T3089
Entity Detected:Fam195a, family with sequence similarity 195, member A ( MGI:1915491)
Sequence:sense strand is shown

>T3089
TGNCTCGAGCAGATTCGGACGAGGCGCACCATGTACACTATCACCAAGGGGCCCAGCAAGCTGGTCGCGC
AGCGCCGCACAGGTGCGCAGGGCGGGCGGGCAGGCGGGACGTCCTGACCGCTGGCTCGCGGCGGCTGACA
GCGCCCTCCGCCCCGGCCCGCAGGTCCCACACAGCAGCAGGTGGAGAGCAGGCTTGGCGAGCTCCTGAAA
TGCCGGCAGCCGGTGCCGCCGACCGCGCTACCCGCGCACCTGCAGCCCTCGGCACAGACTCAGGGACCCT
GGCCCCTTGCAAGTTCTGGGCCAAGACTTGTATTCAATCGAGTAAACGGCCGGCGACCCCTCACCACCTC
CCCATCCCTCGAGGGGACCCAAGAGACCTACACAGTGGCCCACGAGGAGAACGTCCGATTTGTGTCCGAA
GCCTGGCAGCAGGTAGAGCGACAACTGGATGGCGGCCCTGCCGATGAGAGTGGCCCACGGCCTGTGCAGT
ATGTGGAGAGCACTCCTGATCCCCGGCTACAGAACTTTGTACCTATTGACCTCGATGAG
Notes:The probe template was PCR amplified from IMAGE:1546962 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1546962 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:14041 same embryo
 EMAGE:14037 same embryo
 EMAGE:14038 same embryo
 EMAGE:14042 same embryo
 EMAGE:14039 same embryo
 EurExpress:euxassay_001688 same experiment
 MGI:4824733 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS