EMAGE mouse gene expression spatial database

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EMAGE:14115

Pxk PX domain containing serine/threonine kinase ( MGI:1289230)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001792. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001792_01	euxassay_001792_02	euxassay_001792_03	euxassay_001792_04

euxassay_001792_05	euxassay_001792_06	euxassay_001792_07	euxassay_001792_08	euxassay_001792_09

euxassay_001792_10	euxassay_001792_11	euxassay_001792_12	euxassay_001792_13	euxassay_001792_14

euxassay_001792_15	euxassay_001792_16	euxassay_001792_17	euxassay_001792_18	euxassay_001792_19

euxassay_001792_20	euxassay_001792_21	euxassay_001792_22	euxassay_001792_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14115_wholemount_strong.wlz

14115_wholemount_moderate.wlz

14115_wholemount_weak.wlz

14115_wholemount_possible.wlz

14115_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14115_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hypothalamus mantle layer

weak

regional

weak expression: see section 08 09 10 11 12 13 14 15 16 17 18 19

diencephalon lateral wall mantle layer

weak

regional

weak expression: see section 08 09 10 11 12 13 14 15 16 17 18 19

telencephalon mantle layer

weak

regional

weak expression: see section 03 04 05 06 07 08 09 13 14 15 16 17 18 19 20

telencephalon marginal layer

weak

regional

weak expression: see section 10 11 12 13 14 15 16 17 18 19 20

olfactory cortex marginal layer

weak

regional

weak expression: see section 10 11 12 13 16 17 18

hindbrain

weak

homogeneous

weak expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

midbrain

weak

homogeneous

weak expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19

spinal cord

weak

homogeneous

weak expression: see section 08 09 10 11 12 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4409
Entity Detected:	Pxk, PX domain containing serine/threonine kinase ( MGI:1289230)
Sequence:	sense strand is shown >T4409 GCGGCGGGTGGCGGGGCTCGGCGCCTCCGGTTCCTGCCCCGGCTTCAGAGGCAGCGGCGGCGGCGGCCCG GGATGGCCTTCATGGAGAAGCCGCCGGCTGGCAAGGTGCTGCTGGACGACACGGTGCCGCTGACAGCGGC CGTCGAGGCGAGCCAGAGTCTGCAGTCGCACACGGAATATATCATCCGGGTGCAAAGAGGAATTTCTGCA GAAAACAGCTGGCAGATCGTCAGAAGATACAGCGACTTCGATCTGCTTAACAACAGCTTGCAAATTACAG GCCTGAGCCTGCCTCTTCCTCCTAAGAAACTGATTGGAAACATGGACCGTGAGTTCATCGCTGAGAGGCA GCGAGGCCTGCAGAACTACCTTAACGTCATCATGGCAAATCATGTCTTGTCCAACTGTGAGCTGCTTAAG AAGTTCCTAGACCCAAACAACTATTCTGCAAACTATACTGAGATCGCCTTACAGCAGGTTTCCATGTTCT TCCGATCAGAACCAAAGTGGGAGGTGGTAGAACCACTGAAGGACATAGGTTGGAGGATAAGGAAGAAATA TTTCTTGATGAAAATAAAAAACCAACCAAAGGAGCGGCTGGTGCTAAGCTGGGCTGATCTTGGCCCAGAC AAGTACTTGTCAGATAAAGATTTTCAGTGTCTAATCAAACTTCTGCCTTCCTGTGTGCACCCTTACATCT ATCGGGTCACCTTTGCCACGGCTAGTGAGTCCTCCGCATTGCTGATTAGAGCCTTTAATGAGAAGGGAAC TTTGAAGGATCTGATCTACAAGGCAAAACCAAAGGACCCCTTCCTAAAGAAATACTGCAACCCTAAGAAA ACCCAGGGCCTTGAACTCCAGCAAATAAAAACATATGGGCGGCAGATATTAGAGGCACTAAAGTTTCTTC ATGACAAGGGATTCCCTTACGGCCACCTCCATGCAGCCAATGTGATGCTGGATGGCAACACTTGCCGGCT GCTAGACCTCGAAAACTCACTGCTGGGTCTGCCTTCCTTCTACCGATCCTACTTCACACAATTCAGGAAA ATCAATACATTGGAGAGTGTAGATGTGCACTGTTTTGGCCACTTACTCTATGAAATGACTTATGGACGAC CTCCCGACTCCGTGCCTGTGGACTCCTTTCCTCCTGCATCATCCTTGGCTGTGGTGGCCGTATTGGAGTC TACGCTGTCTTGTGAAGCCTGTAAAAATGGCATGCCTACCGTCTCCCGGCTCTTACAGATGCCATTATTC AGTGATGTTTTACTAACAACTTCTGAAAAGCCACAGTTTAAGATTCCCACAAAACTAAAAGAGGCATTGC GAATTGCCAAGGAATGCATAGAGAAGAGACTCACCGAGGAACAGAAGCAGATTCACCAGCATCGAAGACT AACGAGAGCCCAGTCACACCATGGGTCTGAGGAAGAAAGGAAAAGGAGAAAGATCTTAGCTCGAAAGAAG TCAAAACGATCTGCTGTTGAAAACAGTGAGGAGCAGCCAGTGAAGCACAGTAACTCCAATAACTCAGCAG GATCTGGGGCCAGCTCACCTCTCACATCCCCATCATCTCCAACTCCACCGTCCACAGCAGGACTGTCTTC AGCATTACCTCCACCTCCTCCGCCACCTCCTCCTCCACCTCCACCAGCAGGTCCCTCGCCAACCTCAGCC ACGGAGACGCCTGCTCCGTTCCTGCCCCAGCCTGTGAATGGTGTGAACCGTGGGGCCTTGCTCAGTTCCA TCCAGAATTTCCAAAAGGGAACTTTGCGGAAAGCCCAAACCTGTGATCACAGCGCTCCCAAGATCGGCTG ATGCTTCTTGGTAACGCTGAGCAGGCTCAGCCCTCTGCCCCGTCCCACCCCTCTTCCTAGGTGAGCTCTT CCACAGCTTCCCTCAAGGCCTTCCCTGGGTGACTCCCTGCAGAGCCAGCCACACTCCAGTTAGGAGGCTT GAAGAGGGAAGTGCGCTCCAAATAGAATACAGTGGGGCCAGCACTGCTGCCTTAATATTTTGCTCCTTGT GATGCTATTGGGCTTCCTTTAGGGGCAGCACTGTTTGGGTATTATTTTCACCAGCGAAAGAAAAATGTCA GAAAATCAAAAGCAATGGCAGTATTCTGTCACAGCAGCAATTGGCCTTTGTCACTCTGTCCTTCCCACTC TATTATAGAAGTGTTCTCATAGCTCATTCCTCTGCAGCGTCTTTTGGTTAGTTCTTAGGCAGCTCACCTC AGATGCTAACGTTGTAACCTGTCAGTTACACTGCTGAGGGAGAAACAGCGGCTCATCACTCTCCCTACAC AGAGGATGCGGGGGAGGAGACGGGGGAGCTCCTCAGAGCTCAGTGCGGACTGAACTCTGTTCACTGTGCT CAGAACAGCACCTCGGGATCTGTACAGCCACAATGCGCCAGACAGGACATTAACCACAGATAAAGGGTTG TCTTAGTACTCAGCCATTAAATCCAGCTTGGAGTAGAGGGAGGGCCAACATTACAAGTGAATCTGGCATC TATAGTAATCACTTCTGATTTGATTTATCCAACTTAAGCATCTTTTGATATTACTGTTATCAGAAATCTT TTATCCTATGATTTGCTTAAACCTTTTAAATAAATTGCACTTTAAAGGATTATAAAAAATCCATTTTAAA ATTCAAATACACACATCAGTGTTGGTTACTATGCAGAAAGAATGTTATTGTGTATAGATTCATGTAATCT GTGATATATGTTCAGCTGTATTTTTTATTAAAATAAATCATGTCACTAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4922274 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4922274 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14114 same embryo
	EMAGE:14113 same embryo
	EurExpress:euxassay_001792 same experiment
	MGI:4827546 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source