EMAGE mouse gene expression spatial database

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EMAGE:14123

Mettl21a methyltransferase like 21A ( MGI:1914349)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001806. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001806_01	euxassay_001806_02	euxassay_001806_03	euxassay_001806_04

euxassay_001806_05	euxassay_001806_06	euxassay_001806_07	euxassay_001806_08	euxassay_001806_09

euxassay_001806_10	euxassay_001806_11	euxassay_001806_12	euxassay_001806_13	euxassay_001806_14

euxassay_001806_15	euxassay_001806_16	euxassay_001806_17	euxassay_001806_18	euxassay_001806_19

euxassay_001806_20	euxassay_001806_21	euxassay_001806_22	euxassay_001806_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14123_wholemount_strong.wlz

14123_wholemount_moderate.wlz

14123_wholemount_weak.wlz

14123_wholemount_possible.wlz

14123_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14123_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4153
Entity Detected:	Mettl21a, methyltransferase like 21A ( MGI:1914349)
Sequence:	sense strand is shown >T4153 GCAGAGATAGACAGAAGAAATGGCCCTGGTACCCTATGAGGAGAGCGCGGCAATAGGGCTCCAGAAATTC CATAAGCCTCTCGCCACCTTCTCTTTTGCGAACCACACCATCCAGATCCGTCAGGACTGGAGGCAACTGG GAGTTGCTGCAGTGGTGTGGGATGCGGCTGTCGTCCTTTCCATGTATCTGGAGATGGGTGCTGTGGAGCT CAGGGGCTGCTCTGCTGTGGAGCTGGGTGCTGGCACAGGGCTGGTGGGCATAGTGGCTGCCCTGCCGGGT GCTCAGGTGACTATCACGGATCGGAAAGTAGCATTAGAGTTTCTTAAGTCAAACGTTGAAGCCAACTTAC CTCCCCATATCCAACCTAAGGCTGTTGTTAAGGAGCTGACTTGGGGACAAAATTTGGAAAGTTTTTCACC TGGAGAATTTGATCTCATACTTGGAGCTGATGTCATATACTTAGAAGATACCTTCACAGACCTTCTTCAA ACACTGGGACATCTCTGTAGCAACAATTCTGTGATTCTTTTAGCTTGCCGAATCCGCTATGAACGGGATA GTAACTTCTTAACAATGCTGGAGAGGCAATTCACTGTGAGTAAGGTTCACTACGATCCTGAGAAGGACGT ACACATTTACAAAGCACAGAAGAGAAACCAGAGGGAGGACTTGTAGCGGGCAGTATTTTCTAAGAATTGA ATGTACCAGGTAAGGTCTTACAGCTAGAACTCTTAGCCATGTTAATGACGTGGCTGACTGCAAGTCAGTC CGCTTAGAGCTGCTCAAGGAACAAAGCACATGTGTACTTTGTAGTCGGTGCCTTGCCTCATAGCTGTAAC TGTCAGCCAGATTGGCTCACTTCTGCAAGTGGACTTGTGAGGTCTGGCTGGTGTTTCGTTCTTATCCTGT GTGCTCACTGGGTAAGCAGTCCCCACTGATGGAGACTGGTGACCTGGAGAGTCAGTCAGACATTTTGGGA TGGATTTTGTCTCAGTTTCCTTGTCTGCTGAGTGTAAACAGATTTATTACATATATTCCCTGAAATGTGT GTACTGTTGGAGTCTCTTGTGTTTAGTCTTTTTTGTGTCGGCCGTGGGCTGGTCTGAAACCGCAGGCCAG GCTGACCTCAAGCTTGCAGTGACTGTCTTCCCTCTGCCGCCAACTGCTGGGATTACAGGTGTGTGCCATG CCACCTGGCTGACTGTTTAGTTCTTAAGCCCCTTGAGATGAAGATGAGTTCTCTGGGTCTCATGTCATAC CTTTTTACCAGATTTCAGGAGCAAGTATATAAGCAATGAAATAAAAATAAAATTTTACATTTGAAATTAC CTAAACTTGTAGGTTAGAAATTACTTCTAAAACCATGCTTTTGTTTGTTTCAACTTTGCTGTTTCTCAGT TTTGCTGTATCTATTTCTTAGGGTTTTTTTTTTTTTCCTTCTAGTATGTCAGTTTTTCAGAGTTCAATCT CATTTCTTCACAAAGGTCAGCTCCCATGGTGCAGTCTGTATTGTACCTATAATCACCCACTTGGTCTACA GCTCCTGGTGGGCTGCAAGCTGCGGGGAGGCCTTGTAGCTTTGCTTACCCAGGGTCCAGTAAAAACCAAG TAGTCAAGGAGAATTTCAGTTATAGGTAATTGATGTTTCGTGGTGTTAAGGATCAAAACCAGGGTTTTAC AGACCCTCGGCAAACATTAGCCTTGAGTTATTACGTCCCCAGCCAAAGGTTACTAGTTTTTTGTTTTCTC TGGGTAGACTTTATGAATAATGTTATCAGTACAAGCTTCTTCTTTTACATTAAAGCTTGACTAAAAGCTT AAGCAGTTTAGAGCATAGCTATTATAACTGCACAGTGGCAGTGGGTGATAGAAATTTGTTGAATAGACTA TTCAAAGTAAGACCTTTTTTTAAAAATTATTAAATATTTTGGATATAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4483032 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4483032 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14122 same embryo
	EMAGE:14120 same embryo
	EMAGE:14119 same embryo
	EMAGE:14124 same embryo
	EMAGE:14121 same embryo
	EurExpress:euxassay_001806 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source