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EMAGE:14131

Olfr628 olfactory receptor 628 ( MGI:3030462)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:14131 EMAGE:14131 EMAGE:14131 EMAGE:14131 EMAGE:14131
"Pseudo-wholemount" of euxassay_001844. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_001844_01 euxassay_001844_02 euxassay_001844_03 euxassay_001844_04
EMAGE:14131 EMAGE:14131 EMAGE:14131 EMAGE:14131 EMAGE:14131
euxassay_001844_05 euxassay_001844_06 euxassay_001844_07 euxassay_001844_08 euxassay_001844_09
EMAGE:14131 EMAGE:14131 EMAGE:14131 EMAGE:14131 EMAGE:14131
euxassay_001844_10 euxassay_001844_11 euxassay_001844_12 euxassay_001844_13 euxassay_001844_14
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euxassay_001844_15 euxassay_001844_16 euxassay_001844_17 euxassay_001844_18 euxassay_001844_19
EMAGE:14131 EMAGE:14131 EMAGE:14131 EMAGE:14131
euxassay_001844_20 euxassay_001844_21 euxassay_001844_22 euxassay_001844_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:14131Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
14131_wholemount_strong.wlz
14131_wholemount_moderate.wlz
14131_wholemount_weak.wlz
14131_wholemount_possible.wlz
14131_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:14131_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
nucleus pulposus
moderate moderate
regionalmoderate expression: see section 10 11 12
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T5336
Entity Detected:Olfr628, olfactory receptor 628 ( MGI:3030462)
Sequence:sense strand is shown

>T5336
TCCGGCAGTCGATCTCATGCGACTCGGGCTTGGAGTCTGTGCAGTTCTGGATTGGGATTCCTTTCTGTAT
CATGTACATCATTGCACTTTTGGGGAATTCTCTACTCCTAGTTGTCATCAAAGTTGAGCGAAGCCTCCAT
GAACCCATGTACCTCTTTCTAGCAATGCTTGGAGCAACAGATATTTCTCTTAGTACTTGCATCTTACCAA
AAATGTTAGGAATATTCTGGTTTCACCTTCCAACCATATACTTTGATGCCTGCCTCTTACAGATGTGGCT
GATTCACACCTTCCAGGGTATTGAATCAGGCATCTTGTTTGCCATGGCAATGGACCGTTATGTGGCAATC
TGTTATCCTCTAAGACATGCATCCATCTTTACCCAGCGACTCCTCACTCAAATAGGAGTAGGAGTCACAC
TCAGAGCAGCCCTGTTTGTAGCTCCATGTCTCTTTCTCATCAAATGCAGGTTGAAATTCTACTGGACCAC
TGTTGTTTCTCATTCATACTGTGAGCATATGGCCATTGTGAAGCTGGCAGCAGAAGATGTTCATGTCAAC
AAGATCTATGGTCTGTTTGTGGCCTTCAGTATACTTGGACTAGACATAATTTTCATCACACTTTCCTACA
TTAGAATATTTATAACTGTCTTCAAGCTCCCTCAAAATGAAGCAAGACTCAAAGCCTTTAAAACATGCAT
TGCCCATATTTGTGTCTTCTTGGAGTTTTACCTTCTGGCCTTCTTCTCCTTCTTTACACATAGGTTTGGC
TACCATGTTCCATCTACATTCACATTCTCCTGTCCAACCTTTACCTACTTGTCCCACCTTTGCTCATCCT
ATGTTTATGGTGTGAAGACAAACAGATTCTATATGCCCCCCTCTCAAAAAAAAAAAAAAAAACA
Notes:The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:ATCTCAACAGCACCATCTTC; Reverse Primer - name:unspecified, sequence:GAATCTGTTTGGTCTTCACAC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:14132 same embryo
 EMAGE:14136 same embryo
 EMAGE:14133 same embryo
 EMAGE:14134 same embryo
 EMAGE:14135 same embryo
 EurExpress:euxassay_001844 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS