EMAGE mouse gene expression spatial database

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EMAGE:14157

Dgat2 diacylglycerol O-acyltransferase 2 ( MGI:1915050)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003953. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003953_01	euxassay_003953_02	euxassay_003953_03	euxassay_003953_04

euxassay_003953_05	euxassay_003953_06	euxassay_003953_07	euxassay_003953_08	euxassay_003953_09

euxassay_003953_10	euxassay_003953_11	euxassay_003953_12	euxassay_003953_13	euxassay_003953_14

euxassay_003953_15	euxassay_003953_16	euxassay_003953_17	euxassay_003953_18	euxassay_003953_19

euxassay_003953_20	euxassay_003953_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14157_wholemount_strong.wlz

14157_wholemount_moderate.wlz

14157_wholemount_weak.wlz

14157_wholemount_possible.wlz

14157_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14157_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

epidermis

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

nasal cavity respiratory epithelium

moderate

regional

moderate expression: see section 13 16

viscerocranium

moderate

regional

Expression in the turbinate bone.

liver left lobe

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11

liver right lobe

strong

regional

strong expression: see section 12 13 14 15 16 17 18 19 20

bladder

strong

regional

strong expression: see section 11 12

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4939
Entity Detected:	Dgat2, diacylglycerol O-acyltransferase 2 ( MGI:1915050)
Sequence:	sense strand is shown >T4939 CCGCGCTTCGCTGGCTTTCTGCTCATCTAGGGTGGCAGCGGCTACCTACCTCAGCTCTCGCCCTGCTGCC GCCACGGCCTGGGCGCTGTCCCTCAGCTCCCGGAGCTCAGCGCGAAGCCCTGGCCCCGGCGGCCGGGGCA TGGGTCAGGGGCGCGGCGTGAGGCGGCTTTCTGCACGGCCGTGACGTGCATTGGCTTCAGCATGAAGACC CTCATCGCCGCCTACTCCGGGGTCCTGCGGGGTGAGCGTCGGGCGGAAGCTGCCCGCAGCGAAAACAAGA ATAAAGGATCTGCCCTGTCACGCGAGGGGTCTGGGCGATGGGGCACTGGCTCCAGCATCCTCTCAGCCCT CCAAGACATCTTCTCTGTCACCTGGCTCAACAGATCTAAGGTGGAAAAACAGCTGCAGGTCATCTCAGTA CTACAATGGGTCCTATCCTTCCTGGTGCTAGGAGTGGCCTGCAGTGTCATCCTCATGTACACCTTCTGCA CAGACTGCTGGCTGATAGCTGTGCTCTACTTCACCTGGCTGGCATTTGACTGGAACACGCCCAAGAAAGG TGGCAGGAGATCGCAGTGGGTGCGAAACTGGGCCGTGTGGCGCTACTTCCGAGACTACTTTCCCATCCAG CTGGTGAAGACACACAACCTGCTGACCACCAGGAACTATATCTTTGGATACCACCCCCATGGCATCATGG GCCTGGGTGCCTTCTGTAACTTCAGCACAGAGGCTACTGAAGTCAGCAAGAAGTTTCCTGGCATAAGGCC CTATTTGGCTACGTTGGCTGGTAACTTCCGGATGCCTGTGCTTCGCGAGTACCTGATGTCTGGAGGCATC TGCCCTGTCAACCGAGACACCATAGACTACTTGCTCTCCAAGAATGGGAGTGGCAATGCTATCATCATCG TGGTGGGAGGTGCAGCTGAGTCCCTGAGCTCCATGCCTGGCAAGAACGCAGTCACCCTGAAGAACCGCAA AGGCTTTGTGAAGCTGGCCCTGCGCCATGGAGCTGATCTGGTTCCCACTTATTCCTTTGGAGAGAATGAG GTATACAAGCAGGTGATCTTTGAGGAGGGTTCCTGGGGCCGATGGGTCCAGAAGAAGTTCCAGAAGTATA TTGGTTTCGCCCCCTGCATCTTCCATGGCCGAGGCCTCTTCTCCTCTGACACCTGGGGGCTGGTGCCCTA CTCCAAGCCCATCACCACCGTCGTGGGGGAGCCCATCACTGTCCCCAAGCTGGAGCACCCGACCCAGAAA GACATCGACCTGTACCATGCCATGTACATGGAGGCCCTGGTGAAGCTCTTTGACAATCACAAGACCAAAT TTGGCCTTCCAGAGACTGAGGTGCTGGAGGTGAACTGACCCAGCCCGCGCGTGCCAGCTCCTGGGAGGGA CGACTGCAGATCCTTTTCTACCGAGTTCTCGAGTGCATTTTGTTCTGTAAATTTGGAAGCGTCATGGGTG TCTGTGGGTTATTTAAAAGAAATTATAATTTGTTAAACCATTGCAATGTTAGATGTTTTTTAAGAAGGGA AGAGTCAGTATTTTAAGCTCACTTCTAGTGTGTCCTGCTCAAGGTGGAGGCTGATATTTATGGGCCTTGG TGGTTTCTTACCCACCCCTTCTAGCGTTCCCCAGACGACAGACACTTGGCCCTGGCTAGCTGGGCAAGGG CAGTCCTTAGTGACTCCAGGGATTCTTGAGAGGCAGAGGCCATGTCCCACCCGTGGCTGCAGGTCGGGTT CCTCGTACCAAGGGGAGGCTGAGGGCACAGCTGGCCCCACTTGGGGAGGGTAGATAACATCTGGACTGCC CGGCTTGGGTCTCTGCTCCTCACCCTAGCCCTCTTCTCCAATCTGAGCCTACCCTGGCCTCCTGTCTCCT GGCTAGGGACACGGCTGTCCCACAGGTGCCGTCTTGGGTTATCTCGCTGCTGTTGGCTGGTTTCACTCTG GAGGTTGGCACCATGGACACAGCTCAGCGTTGCTCTGGCGCATATCCTCCTGAGCCACACCCCAAGTCTG GTGTGAGGAAGGGCTTCTCTTCTCTTCACAGAGGTGCCTGGCTTCTTGTGCAGCACACTGGGTCCAGGAC AGGAGGACCCCCCCAAACCAAGCCTCACGTGTGTGCCTTTATGAGGCGTTGGGAGAAAGCTACCCTCCTG TGTATTCTGTTTTCTCCATGAGATTGTTGTGCCATGTCACACTTTTGTATATTCCTAGACTAATAAATGG AAACAAGAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5125951 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5125951 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14156 same embryo
	EurExpress:euxassay_003953 same experiment
	MGI:4824286 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source