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EMAGE:14192

Edf1 endothelial differentiation-related factor 1 ( MGI:1891227)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:14192 EMAGE:14192 EMAGE:14192 EMAGE:14192 EMAGE:14192
"Pseudo-wholemount" of euxassay_004011. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_004011_01 euxassay_004011_02 euxassay_004011_03 euxassay_004011_04
EMAGE:14192 EMAGE:14192 EMAGE:14192 EMAGE:14192 EMAGE:14192
euxassay_004011_05 euxassay_004011_06 euxassay_004011_07 euxassay_004011_08 euxassay_004011_09
EMAGE:14192 EMAGE:14192 EMAGE:14192 EMAGE:14192 EMAGE:14192
euxassay_004011_10 euxassay_004011_11 euxassay_004011_12 euxassay_004011_13 euxassay_004011_14
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euxassay_004011_15 euxassay_004011_16 euxassay_004011_17 euxassay_004011_18 euxassay_004011_19
EMAGE:14192 EMAGE:14192 EMAGE:14192 EMAGE:14192
euxassay_004011_20 euxassay_004011_21 euxassay_004011_22 euxassay_004011_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:14192Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
14192_wholemount_strong.wlz
14192_wholemount_moderate.wlz
14192_wholemount_weak.wlz
14192_wholemount_possible.wlz
14192_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:14192_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T5009
Entity Detected:Edf1, endothelial differentiation-related factor 1 ( MGI:1891227)
Sequence:sense strand is shown

>T5009
CGAGCAGCTGCCAGAGCCGCCGGACGGATCGCTGCCCCCGCCGCGCCATGGCCGAGAGTGACTGGGACAC
CGTGACGGTGCTGCGCAAGAAGGGCCCCACGGCCGCACAGGCCAAGTCTAAGCAGGCCATCTTAGCGGCT
CAGCGACGAGGAGAAGATGTGGAGACATCCAAGAAATGGGCTGCTGGCCAGAACAAGCAGCATTCCATTA
CTAAAAACACAGCCAAGCTCGACCGGGAGACCGAGGAGCTGCATCACGACAGGGTGACCCTGGAGGTGGG
CAAAGTGATCCAGCGGGGCCGGCAGAGCAAAGGCCTGACCCAGAAGGACCTGGCCACGAAAATCAATGAG
AAGCCGCAAGTCATCGCAGACTATGAAAGTGGACGGGCCATTCCGAATAACCAGGTTTTGGGCAAAATTG
AGAGAGCCATCGGCCTCAAGCTCCGGGGGAAGGACATCGGGAAGCCTATCGAGAAGGGGCCGAAGGCGAA
ATGAACACAAAGCCTCGAAGCTGTGGTTCCGGCTGAGCTCTCCTGGCTGGTCCCCCAGGACCACCAGCAC
TGCCGTGGGTCTCCTCACATGGTCAAACGGAACACGGGGGGTTAGGCCTGTGTGCCCCCTCAACCCATAA
CTGCTGTCAAACAAAGCAAACCTTGTAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:5250117 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5250117 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:14193 same embryo
 EMAGE:14191 same embryo
 EMAGE:14194 same embryo
 EurExpress:euxassay_004011 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS