EMAGE mouse gene expression spatial database

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EMAGE:14238

Bcl2l11 BCL2-like 11 (apoptosis facilitator) ( MGI:1197519)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001763. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001763_01	euxassay_001763_02	euxassay_001763_03	euxassay_001763_04

euxassay_001763_05	euxassay_001763_06	euxassay_001763_07	euxassay_001763_08	euxassay_001763_09

euxassay_001763_10	euxassay_001763_11	euxassay_001763_12	euxassay_001763_13	euxassay_001763_14

euxassay_001763_15	euxassay_001763_16	euxassay_001763_17	euxassay_001763_18	euxassay_001763_19

euxassay_001763_20	euxassay_001763_21	euxassay_001763_22	euxassay_001763_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14238_wholemount_strong.wlz

14238_wholemount_moderate.wlz

14238_wholemount_weak.wlz

14238_wholemount_possible.wlz

14238_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14238_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

renal cortex

moderate

homogeneous

moderate expression: see section 09 10 11 17 18 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4117
Entity Detected:	Bcl2l11, BCL2-like 11 (apoptosis facilitator) ( MGI:1197519)
Sequence:	sense strand is shown >T4117 CTCCCCGCAGGCACTGGGTCACCAGCTGGTTGGAGCTCTGCGGTCCTTGCCATCGTCGCCGTCACCGGAT TCCTGCAGCCGCTCTGCGAGCTGTGTTCCACTTGGATTCACACCACCTCCGCTGCTCCCGCCGCCACCGC CTCGGCGCCCTTCCCCTGGCCCTCGTCCACCCAATGTCTGACTCTGATTCTCGGACTGAGAAACGCAAGA AAAAAAGACCAAATGGCCAAGCAACCTTCTGATGTAAGTTCTGAGTGTGACAGAGAAGGTGGACAATTGC AGCCTGCTGAGAGGCCTCCCCAGCTCAGGCCTGGGGCCCCTACCTCCCTACAGACAGAACCGCAAGGTAA TCCCGACGGCGAAGGGGACCGCTGCCCCCACGGCAGCCCTCAGGGCCCGCTGGCCCCACCGGCCAGCCCT GGCCCTTTTGCTACCAGATCCCCACTTTTCATCTTTGTGAGAAGATCTTCTCTGCTGTCCCGGTCCTCCA GTGGGTATTTCTCTTTTGACACAGACAGGAGCCCGGCACCCATGAGTTGTGACAAGTCAACACAAACCCC AAGTCCTCCTTGCCAGGCCTTCAACCACTATCTCAGTGCAATGGCTTCCATACGACAGTCTCAGGAGGAA CCTGAAGATCTGCGCCCGGAGATACGGATTGCACAGGAGCTGCGGCGGATCGGAGACGAGTTCAACGAAA CTTACACAAGGAGGGTGTTTGCAAATGATTACCGCGAGGCTGAAGACCACCCTCAAATGGTTATCTTACA ACTGTTACGCTTTATCTTCCGTCTGGTATGGAGAAGGCATTGACAGGATCTACATGCAGCCAGGATACGT GGCGGACATGGCTCTTGTTCAGACTGGGAGAACCCCCACGCGTCATGTCCCTCTCTTGGTGCTGCGACAG TGTGTCCAGTGGTTCTATCCCAGAGAGATGTGCTGAGCATGGACAGCGCTCTGCACTGTGTCGATGTGAA CGGAACCTCTGTTCATCACCACATGGCCGAGTTTTCAGTAAATATTTGTTGTGAATGTAAACAAGGGAGG GCTTTTCTCTTTTTAATGTACAGATCCTAGGAACAGAGAAATATGCAAGAGAGGTGTTTAAAAAAAAAAA AAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4459720 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4459720 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14237 same embryo
	EMAGE:14241 same embryo
	EMAGE:14240 same embryo
	EMAGE:14239 same embryo
	EMAGE:14236 same embryo
	EurExpress:euxassay_001763 same experiment
	MGI:4823450 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source