EMAGE mouse gene expression spatial database

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EMAGE:14266

Dnajc22 DnaJ (Hsp40) homolog, subfamily C, member 22 ( MGI:1920028)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003987. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003987_01	euxassay_003987_02	euxassay_003987_03	euxassay_003987_04

euxassay_003987_05	euxassay_003987_06	euxassay_003987_07	euxassay_003987_08	euxassay_003987_09

euxassay_003987_10	euxassay_003987_11	euxassay_003987_12	euxassay_003987_13	euxassay_003987_14

euxassay_003987_15	euxassay_003987_16	euxassay_003987_17	euxassay_003987_18	euxassay_003987_19

euxassay_003987_20	euxassay_003987_21	euxassay_003987_22	euxassay_003987_23	euxassay_003987_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14266_wholemount_strong.wlz

14266_wholemount_moderate.wlz

14266_wholemount_weak.wlz

14266_wholemount_possible.wlz

14266_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14266_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

midbrain mantle layer

moderate

regional

moderate expression: see section 08 09 10 19 20

stomach

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11

hindgut

moderate

regional

moderate expression: see section 14 15 16

midgut

moderate

regional

moderate expression: see section 09 10 11 12 13 14 15 16 17 18

liver left lobe

moderate

regional

moderate expression: see section 01 02 03 04 05 10 11 12 13 14 weak expression: see section 06 07 08 09

liver right lobe

moderate

regional

moderate expression: see section 15 16 weak expression: see section 17 18 19 20

kidney calyx

moderate

regional

moderate expression: see section 09 10 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4972
Entity Detected:	Dnajc22, DnaJ (Hsp40) homolog, subfamily C, member 22 ( MGI:1920028)
Sequence:	sense strand is shown >T4972 GGTGAGAGCAGTTACAGAGGGGCCATTTTGAATCTGTGTTCAAAGGCAATGGCTGTTCTGAAGCAGCCTG GAAGCCTGGGCTGCCAGGTACTGCGGCCCTGCTGGGACTGCCTGAGGGCCTAAGGATGACCCTCCAGACT GCAGTGATTCTCACAGAGGACCTTGAGATTTCTGCAATTTGATCAATGTCAAACGCCACTGGATTTTCTC TGAGACTCTTGCCCTAGAGGATGGCCAAAGGGCTGCTGATGACCTATGTCCTTTGGGCTTTGGGGGGTCC TGTTGGGCTCCACCACCTGTACCTGGGAAGGGACAGCCATGCCCTGCTCTGGATGCTCACCCTGGGAGGT GGTGGGTTGGGCTGGCTCTGGGAATTCTGGAAGCTCCCGAGCTTTGTGGCTCAAGCCAACAGAGTCCAGA GTTGGAAACAGAGGCCGGAAGAGGAGAGACCACCTCTCAGTCTCCTTCGCTTTGCTTCCCAGATAGTAGT GGGTGTCTATTTTGGTCTTGTGGCTCTAGTCAGCCTTTCTTCCATGGACAACTTCTATATAGTGGGCCTC CCACTGGCTGTTGGCTTAGGGGTCTTGCTGGTAGCTGCTGTTGGTAACCAGACCTCAGACTTTAAGAATA CCTTAGGAGCGGCCTTCCTCACGTCCCCTGTGTTCTATGGCCGCCCCATAGCCATCCTGCCTATCAGTTT GGCTGCCAGCATTACAGCCCAGAAGCATCGACGATACAAAGCCTCAGCGGGGTCAGAGACACTTAGTGTG CGGCTCTACCGCGTGGGCTTGGCTTACCTCGCCTTCACCGGCCCTCTGGCCTACAGTACGATGTACAACA CAGCTGCCACCATTAACTACGTGGCAGAAACCCTGGGTTCCTTCTTGAGTTGGTTCAGCTTTTTCCCACT TCTTGGCCGCCTCGTGGAGTCTGTCTTGCTTCTGCCTTGCCGGATCTGGTGGCTACTGGTCGGAGGTCCT GGCTTCAACAGCAGCCGGTTCCAGGAGTGGGAAAAGCTCTATGAGTTTGTCGACAGTTTTCAGGATGAGA AACTTCAGCTGGCTCACCAGGTTCTGGGCGTCCCAGAAGGAGCAACTAATGAAGAAATACATCGGAGTTA CCGGGACCTGGTGAAAGTCTGGCACCCAGATCACAACCGGCACCAGACGGAGGAGGCCCAGAGACACTTC CTGGAGATCCAGGCTGCGTATGAAGTCTTGAGTCAGCCCAAGAAGCCTAGAGCATCCTAGAGGTGAAAAG CAACCACTTGAGGATGGACTCAGTCTTGTCAGCACCATCCTCAAAACCCAGGACCTGACAGTTAAAGAAA CTGCTGGCAGCAGAGGGGAGGGAAACCTTACAGGGATGAGAAGGGTAGAGAATATATTTATGTGTTGAAT TCTTGACTACCGTCTCCTCGATTATACTCTCTTAAGACGGAAAAGTAAAAGGAATATTATAAAAAAAAAA AAAAA
Notes:	The probe template was PCR amplified from IMAGE:5136672 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5136672 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14263 same embryo
	EMAGE:14267 same embryo
	EMAGE:14264 same embryo
	EMAGE:14268 same embryo
	EMAGE:14265 same embryo
	EurExpress:euxassay_003987 same experiment
	MGI:4824355 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source