EMAGE mouse gene expression spatial database

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EMAGE:14323

Prkab2 protein kinase, AMP-activated, beta 2 non-catalytic subunit ( MGI:1336185)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004124. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004124_01	euxassay_004124_02	euxassay_004124_03	euxassay_004124_04

euxassay_004124_05	euxassay_004124_06	euxassay_004124_07	euxassay_004124_08	euxassay_004124_09

euxassay_004124_10	euxassay_004124_11	euxassay_004124_12	euxassay_004124_13	euxassay_004124_14

euxassay_004124_15	euxassay_004124_16	euxassay_004124_17	euxassay_004124_18	euxassay_004124_19

euxassay_004124_20	euxassay_004124_21	euxassay_004124_22	euxassay_004124_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14323_wholemount_strong.wlz

14323_wholemount_moderate.wlz

14323_wholemount_weak.wlz

14323_wholemount_possible.wlz

14323_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14323_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

medulla oblongata basal plate mantle layer

moderate

regional

moderate expression: see section 09 15

trigeminal v ganglion

moderate

regional

moderate expression: see section 06 07 08 15 16 17 18 19

spinal cord

moderate

regional

moderate expression: see section 09 10 13 14 15 16 weak expression: see section 11 12

dorsal root ganglion

moderate

regional

moderate expression: see section 09 10 14 15 16 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5637
Entity Detected:	Prkab2, protein kinase, AMP-activated, beta 2 non-catalytic subunit ( MGI:1336185)
Sequence:	sense strand is shown >T5637 GGGGTGTCTTTTCTCCTAGTGTGACCCAGCCTTACTTCCTGAGCCCAATCATGTTATGCTGAACCATCTC TATGCACTGTCCATTAAGGACAGTGTGATGGTCCTTAGCGCAACCCATCGCTACAAGAAGAAGTATGTCA CCACGCTGCTGTATAAGCCCATCTGAGGAAGGCGTCACGCCTTGCCCCCAAGGATTCTGGAGAAGCATCT CCCTTGCCTTTGAACTGAGCTGGCCTCCTCTGAGGCTGGCAGGCTGGTTTGTTTTGAGGCTGCTGTGTGT GTCAGAGCCTCTGATAGGATGCTCTGCTTTGCATTTGATTGCAGATGAGAGCTTTATGAGTTCACGGAGT TTATTTTAAGAAACACAAAACCATATATATGCATATGAGAGAAAGGTTACCGGAAGTCTCCTGGCCCCGC TGTACAGGTTCTCTCTGCCTGTGGGTGGTCGCCAGGGCCTGACTGGGAAGCAGTGGCCACAGGAATGGGC ATCCCAGAAGCTTCTTTTTCTAAAGTGAGAGAATAGCAAACTCTTAGGATCTTTGTTGGGCAGAGGTTCT GGACCACTGGTGTCCAGGGGTGAGATGTGTGCCAGGCTGCTGCCCTCCTTAGAGCTGCCTCAGTGCAGGG TGGCTTTCCTTGCATGGACTGATTCTGTATTCCCACACTATGTGACACAATCTCCTGTGTGTTTATATGA CACTAGATGCCCCACAAGAACCCTCTTCATTTTATGTACCCAATTTAATAAGTCCCCTCAGATCTCATAC ATGGCCCAGTAATGCAAAACTCACTGGGCAAGGGACCCTTCTGAAAAGTCTGTGTGATTAGACTCTCCTG AGAGACTAGAGGTGCCAGGGTCTCCTTTTTAGCTCAGCGAAGGCCTAACCCAGTGTGTTACAATTTATTT TAACATCTAGGGGAAAATGAAGCTCTTTTTGTTTCAGTTTGGTCCTGTATATTGGCTGTGTGAGCCTGTT TACGGTTTTTCTTTATCAGAGCATTCAGTTTGTAGATAGGTTTGGAATTAGGGAAAACTTCCGTATCTTT CATCCCTGAAAGATTTTTTTTTTATGTGCCTACATTTTTCTTTAATTAAAAAAAAAAAGCTTATCCATCC ATGTTAACAGGCTGTTAAAGGACCTCAGGCTTTTGACAAATGGTTCTTAAGCTTTTCTTCTCTAGTCTAG GCAATTCTACAGTCTTGGGTTAATATGGCCAGCCATGTGTACCTCGCTACCCAAGCACAGCTCTAGACAC AAGTTCATGCAGACTCACTGGATGCTCATGCAGGCTCACTGGGTGCTCATGCGAGGTTTCCCTACCATTA AAGATGTGTGAGGCAAGGAACTACCCTCGGTGTTCATAACCGTGCACAGTATTGCTCTTTGCACCAAATA TTAACAAAGTATTTTTTTCTTTCGCCTCTTCCCTTTAAATGTGTTTGATGATGGAATGGGGTCACTGATA ACCACTGACTGAGAAGTATTCCAACCTACTCAGGATTGAAAGGAAATATTTTTTCCAGTATCTTGACACT TCCATTTTACAGATAAATGTAAGTCAATCATTAGGCACTGTTTCTGGTTGAGAGCCTTCCTGTCTCCTGA GATGAGCCAGCCACATTGAAAGGGACCTGCTGGTCAGTTCACCTACCTCCTGGCTCTCCCACATGAGGGT GGCACAGCAAGTCCTCTGCACACCTCTGAGGCCTCTTATCACAAGGGTGAATAGGAAGATGGGGAGGTTT CTGTCCTGCAGGGAGTACAGATCGCTCAATGCCTTGGTACATGAGTCCTGGGAGATGCCGCTTCAGCCAT CCCAAGCCACCTCCCTCAGCTGAGACTCTGTGGAGAACCCTCGTGGTCCAGTCAGCTTTCTGAATAGTGT TCCATTTTGAAAGCCAAGGCCTCATCTTGGTTTTTCATCAACTCTAAGGCCCTTCAGTTATGTCGAAAGG TTTTATTTATTTTTTCTGTTTTTCCTCAGTGCTGATTTAGAAGTGGGCCACCCAGGAGGATTCAAAAGCC TTGCTTGTTTCCCAGTAGGATTGTTGTATCTACACTTCCTGTTAACAGTCTAAGCTGGGATCATCTGTGT TTGTGCATTAAGTTTTGACCCCTCAAAGTTGTAGCTATGGAAGGGTGGGGAAAGCTGTCTTCAGTCTCTC ACTCAGGCTGATGCTTCTCTGACGGGTGAGGCTGTGGTGATAGAGGGGTGGGTGCTGATGGCCTCATCTG GAAATGCAGGAGAGAGTTAGAAGGGCTGGCTGATAGCTCACCAGCACTGTCCCTGTCCTGGCCCCTGACC CTGGGGAATGCTGCCCCTGTCACCAGGAAACACTTGTAAAGAGACAGAGTGAGTACCGCTTTTCTTTAGT TACTTTAAGGGGCAGTGTGTTTTCTACAATAAAAAGTCGGACAGAATTAAGAAACAAATCAAGGCAAAAC TATTTCTTTTTCTTTCCCACCTATGGGAATTTTGCAGGGTCAGTTGGTAGTAAAACGGCCCTTAAGGAGG CTAACACAACACCTGTAGTGTAGATCAGTGCCTGTGAGGCCAGGGAAGCAGCGAAGGAGCCCAAGGGAGA TAGCAGGAACTGAAGGCTCAGGGAGCTTGGCACTGAAAATGTCCCCTTAAAGAGTTCAAGCAGACATCAC CTGGTACAAACCACTTAGCAGCCCAGAAACTGTCTTCTTACCCTGTTCTCACCCTTTCCTCTGCGTTCTG TCTGACTTCACTTATCAGTAAGACAGGTTCACCAGAGTCTCAAGGAAAAAGACCTAGACAGTGTGAAGAG CTTTCACTCGAGCCATGGTTATTAGCCTCCTGTCAACCTAGACCTGAGTAGGATTCACACCTCTGAAGGG CTCAGCTAGCTTCTTCAAGAGCCCTGGCCTCTTGGGCCGGAAGTACAAAGGAAGAAAACCCGTTTCTGAG AGAAACCTTCGCCCAGCCGTCCCTGCTGGTTTTGGTTTTTTGTAGATCTAATGGATCTTTCATAAACCTT TTGAAAAGTGTCACTATTGCATGCATGCTGTTAAAGTGATGTGACGTGGAAGTGGGAACAGGGCCTTTAA ATGTTTGACCTCAAAACAATATGCAATAGTCCCTGTGGGTGTGTGTGTGTGTGCACATATGTGTATGTGG TATTCCTTCTGTTCTCAGAGATACTTAGTCCTCATATTCTTGTAACCTGGGGCTTAAGAGCAGATAAATA TTTTGTTGGTGTGTTATGACTAATCCTCAAAGCCAAGTTCTTGCTGTCTTCTGTCAGCGGCAGACTCCAG TTACTCATGAATATGAGACCAGGAAGAAATAAGGGTCTTTCAGGACTCTGGATCTAGGCATTTGTCTGTC CTTAAGTGACAATCACAACTGGAGATTGAAAGACTGATTTCTGCAGTGAACAAACATGTTTGGAGTAAAA AAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6494114 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6494114 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14326 same embryo
	EMAGE:14327 same embryo
	EMAGE:14325 same embryo
	EMAGE:14324 same embryo
	EurExpress:euxassay_004124 same experiment
	MGI:4827415 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source