EMAGE mouse gene expression spatial database

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EMAGE:14415

Vmp1 vacuole membrane protein 1 ( MGI:1923159)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004235. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004235_01	euxassay_004235_02	euxassay_004235_03	euxassay_004235_04

euxassay_004235_05	euxassay_004235_06	euxassay_004235_07	euxassay_004235_08	euxassay_004235_09

euxassay_004235_10	euxassay_004235_11	euxassay_004235_12	euxassay_004235_13	euxassay_004235_14

euxassay_004235_15	euxassay_004235_16	euxassay_004235_17	euxassay_004235_18	euxassay_004235_19

euxassay_004235_20	euxassay_004235_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14415_wholemount_strong.wlz

14415_wholemount_moderate.wlz

14415_wholemount_weak.wlz

14415_wholemount_possible.wlz

14415_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14415_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6105
Entity Detected:	Vmp1, vacuole membrane protein 1 ( MGI:1923159)
Sequence:	sense strand is shown >T6105 CCAGCGGCGGGTGTGAGAGTCGGTGTTGTGAGCCGCTTCCAGGATCCTGCGATCCGGAGAAGCCGGGGTC TGAGCGGCTCCTCCAGAGTTCCTGATCTATGAAATGGCAGAGAATGGAAAAAATTGTGACCAGAGACGCG TAGCAATGAGTAAGGATCAGCACAATGGAAGTCTCACAGACCCCTCTTCAGTTCATGAGAAGAAGAGAAG GGATCGGGAAGAAAGACAGAATATTGTCCTGTGGAGACAGCCACTCATTACCTTGCAGTATTTCTCTCTG GAAACTCTTGTAGTTTTGAAGGAATGGACCTCAAAATTGTGGCATCGTCAAAGCATTGTGGTGTCCTTTT TACTGCTGCTTGCTGCGCTTGTAGCTACGTATTATGTGGAAGGAGCGCACCAACAGTATGTGCAGCGGAT AGAGAAGCAGTTTCTTTTGTATGCCTACTGGATAGGCCTGGGGATTTTGTCCTCTGTTGGTCTTGGAACA GGACTGCACACCTTTCTGCTTTATCTGGGCCCACATATAGCTTCAGTTACATTAGCTGCTTATGAATGCA ATTCGGTGAATTTCCCTGAGCCACCCTATCCTGACCAGATTATCTGCCCAGAGGAAGAAGGCGCTGAGGG AGCCATTTCTTTGTGGAGTATCATCTCAAAAGTTAGAATTGAAGCCTGCATGTGGGGCATTGGAACAGCC ATTGGAGAGCTGCCTCCATATTTCATGGCCAGGGCAGCTCGCCTCTCAGGTGCTGAACCAGATGATGAAG AGTATCAGGAATTTGAAGAAATGCTGGAACATGCAGAGGCTGCACAAGACTTTGCATCACGGGCTAAACT GGCAGTTCAAAAACTAGTACAGAAAGTTGGATTTTTTGGAATTTTGGCCTGTGCTTCTATTCCAAACCCC CTGTTTGACCTGGCTGGAATAACGTGTGGGCACTTCCTTGTACCTTTCTGGACCTTCTTTGGTGCAACCC TGATTGGGAAAGCAATCATTAAAATGCATATCCAGAAAATATTTGTTATAGTAACTTTCAGCAAGCACAT CGTGGAGCAGATGGTGACTTTCATTGGTGCTGTCCCTGGCATAGGTCCGTCTCTGCAGAAGCCTTTTCAG GAGTACCTGGAGGCGCAGCGGCAGAAGCTTCATCACAGAAGTGAAGCGGGCACACCGCAGGGAGAAAACT GGTTATCCTGGATGTTTGAGAAGCTGGTGGTTGCAATGGTGTGTTACTTTGTCCTGTCTATTATTAACTC CATGGCACAAAACTATGCCAAACGAATCCAGCAGCGCTTGAACTCAGAGGAGAAAACTAAATAAGCAGAG AGTTTTTATCTGCAGAAGTTAACATGGTGGAGTCCTGCCTTACACTGGGAGGGCTCTAACCAGGAAGGAA GGTCCCCGTTTCCAACCTGTGTTGATTTTTAAAACATTCTTCCTGAAAGCAGTTTAGTCCATATGTTACA CTGACATACTTTATTTGTTAAGGTAAGGTGTCTACACCCTCAGTTCAATCCACGTCGTATTCTTTAGGGT GGATGTGATGTTCTGATGCAAACTTAACAAAAACTGGCCTTCTGATACCTTCACAGGGCCACACAGAACC TTCTGAAGTATGTTAAATATGTCAGGCTTTTTAGGCTTGTCACAAATGATTGTTTTTTTCTAAGTCACCA AATGTATATAAGTTATATATATTGGATAGCAATCTTGCATGTCTATCATGGAACAATTTAATATGCCTTC CTTTCCCACCCTCAAAAAGGCCATTTTATGACGCATTGCACACCCTCTGGGGAAATTGATCTTTAAATTT TGAAACAGTATAAGGAAAATCTGGTTGGTGTCTCACAAATGAGTACGCCATTTTTTATTCTGTATATTTA GAATGAAGTCGTGAAAAACTTTATAAAGACATCTTTGATCATTCCGAAAAAAAAAAAAAAAAAAAAAAAA A
Notes:	The probe template was PCR amplified from IMAGE:3493738 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3493738 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14414 same embryo
	EMAGE:14413 same embryo
	EMAGE:14417 same embryo
	EMAGE:14416 same embryo
	EurExpress:euxassay_004235 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source