EMAGE mouse gene expression spatial database

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EMAGE:14431

Zfp454 zinc finger protein 454 ( MGI:2679253)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004237. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004237_01	euxassay_004237_02	euxassay_004237_03	euxassay_004237_04

euxassay_004237_05	euxassay_004237_06	euxassay_004237_07	euxassay_004237_08	euxassay_004237_09

euxassay_004237_10	euxassay_004237_11	euxassay_004237_12	euxassay_004237_13	euxassay_004237_14

euxassay_004237_15	euxassay_004237_16	euxassay_004237_17	euxassay_004237_18	euxassay_004237_19

euxassay_004237_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14431_wholemount_strong.wlz

14431_wholemount_moderate.wlz

14431_wholemount_weak.wlz

14431_wholemount_possible.wlz

14431_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14431_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7903
Entity Detected:	Zfp454, zinc finger protein 454 ( MGI:2679253)
Sequence:	sense strand is shown >T7903 CAAGCTTCTAAGGGAAGCCCCACGGTGATCTGCAGTGTCCAGAGACTGAAGGTCAGGTCTGGGAGTTGAG AACAGAGGGAGTGCAGATGCTTCTAGCCAGAGAACCCCAGCAATTGTAAAGCTCCTCCCCTGCCTCCTTC CTACTCAATGCTCCTCAGCTCACCAGAGAAAAGGAATGGCTGTCAGCCAGCTCCCGGCCCTGGTCCAGGA CTTGGTGACCTTCAAGGATGTGGCCGTGCTTTTCACCCAGGAGGAGTGGGGTCAGCTGAGCTCTGCCCAG AGGGCCTTGTATCAGGATGTGATGCTAGAGAACTACAGTAACCTGGTCTCACTGGCAGGTCTCTTAGGAT CCCAACCAGATATGTTCTTTCCCCTGGAGGAAGTGGAAGAATGTGTGTCAGAGGAGGCTCCTGAGGGCTT TGTTCTGGATGCCGCAGATGAACCTCACTTGGGTCCTCGATCTGCGTGTGAAGCTCAGGTTCCGGTTACA GGGGAAATTACAGTGGCCATGAGTAAGAAATTCCTCAAGGCAGATATCCCTGAGAAAGAGTCGGATCAAT GGATAGCAAAGGAAAGAGCATGTGCTAACTTGTTCAATTGGCAGTGCCAGGAAGGTCAGGAGGTGAAGTT GCAGCAAGTGGTCCTCACCCAGCAGAACACTCCATCAAAACTGAGTGAGCAGAGAGTCAGAGCAGAGAGC TCATCACCCATCCAAAGTCAGAGATCTCAAGCAAGCAAAACGGCTTTTGAATGCAGCGAATGCGGGAAAG CCTTCTCTAAGAGTTCCACCCTTAAGAAGCATCAGAAACTTCATACCGAAAAACTCAACCCAAGTCAGAA ATCACCAATGAAAGAGAAGCGATACAAGTGCCGAGAATGTGGGAAAGCCTTCCACCAGAGTACACACCTC ATCCATCACCAAAGAGTCCACACTGGCGAGAAACCATACCAGTGCAAGGACTGTGGCAAGGCCTTCTCAG TGAGCTCTTCCCTTTCCTACCACCAGAAAATCCATACCGGAGAAAAGCCCTTTGAATGTAATGTTTGTGG AAAGGCCTTTATCCGGAACATACATCTTTCCCACCATCATAGAATGCACACTGGAGAGAAACCTTTCCAA TGCAACCTTTGTGACAAAGCTTTCGTTTGCAGAGCACATCTTACCAAACACCAGCACATTCACAGTGGCA AGAAGCCCTATAAATGTAATGAGTGTGGGAAAGCCTTCAATCAGAGTACAAGTTTTCTGCAGCATCAGAG GATCCACACTGGAGAGAAACCCTTTGAATGCAACGAGTGTGGCAAGGCCTTCAGGGTGAACTCTTCCCTT ACTGAACACCAGAGAATCCACACTGGGGAGAAACCATATCAATGTATCGAATGTGGCAAAGCTTTCAGGG ATAACTCATCCTTTGCACGCCATCGGAAAATTCACACTGGAGAGAAACCTTACAGATGTGGCTTGTGTGA GAAGGCTTTCAGGGACCAGTCAGCCCTAGCACAGCATCAGAGAACTCACACTGGGGAAAAGCCTTACACG TGTAACATATGTGAGAAAGCCTTCAGTGACCATTCGGCCCTTACCCAACATAAGAGAATTCACACGAGAG AAAAACCGTACAAATGTAAAACCTGTGGGAAAGCTTTTATCCGAAGCACACACCTCATTCAACACCAGAG GATTCACACGGGAGAGAAACCTTACAAATGTAACACATGCGGGAAAGCCTTCAACCAGACTGCAAACCTC GCTCAGCACCAGAGGCATCACACTGGAGGAAAGTGATAGAAAGGCAGTTAATAGGTGCTGAGGGGATGAT GTCATTAATTATTGAATAGAAGAGAGTACATAATCACACTGAAGTCTTTTATCATCATGGTTGGATTTAC TCGGAGCCAGGTTGCCTAATGCTAAGGGTCTTGAGAATCTTATGAATGGTGAACACACCTTACTCTTCAG TCTGCCACAGTTGGATAGTAGTGCCTTTTTATTGGCCTGAATTACTATTAGAACCAAAATGAAAATTCAT TAAAAGCATAAATGTCAGTTTATCAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6510760 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6510760 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14433 same embryo
	EMAGE:14432 same embryo
	EMAGE:14430 same embryo
	EMAGE:14435 same embryo
	EMAGE:14434 same embryo
	EurExpress:euxassay_004237 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source