EMAGE mouse gene expression spatial database

EMAGE:14488

Trappc2l trafficking protein particle complex 2-like ( MGI:1916295)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005340. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005340_01	euxassay_005340_02	euxassay_005340_03	euxassay_005340_04

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euxassay_005340_10	euxassay_005340_11	euxassay_005340_12	euxassay_005340_13	euxassay_005340_14

euxassay_005340_15	euxassay_005340_16	euxassay_005340_17	euxassay_005340_18	euxassay_005340_19

euxassay_005340_20	euxassay_005340_21	euxassay_005340_22	euxassay_005340_23	euxassay_005340_24

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Expression Pattern Description

Spatial Annotation:

Annotation colour key:

wholemount mapping

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Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8268
Entity Detected:	Trappc2l, trafficking protein particle complex 2-like ( MGI:1916295)
Sequence:	sense strand is shown >T8268 GCACAGCCTGCCAATATGGCGGTGTGCATCGCGGTGATCGCCAAGGAGAATTACCCTCTGTACATCCGAA GCACTCCGACAGAGAGTGAGCTCAAGTTCCACTACATGGTGCACACGTCTCTGGATGTGGTAGACGAGAA GATCTCTGCCATGGGGAAAGCACTGGTGGACCAGAGGGAGCTGTACCTGGGCCTGCTCTACCCCACCGAG GACTACAAGGTATATGGGTATGTGACCAATTCCAAGGTGAAATTTGTCATGGTCGTGGATTCCTCCAATA CAGCCCTTCGGGACAACGAGATCCGCAGTATGTTCCGGAAGCTGCATAACTCTTACACAGATGTGATGTG CAACCCCTTCTACAACCCGGGAGACCGCATCCAGTCAAGGGCCTTTGATACCATGGTGACTTCCATGATG ATCCAGGTGTGCTGAGTGGGGACCTCTGCCTGCCTCAGTCGTTCAGAAGAGAAGCCTGCGTTCACCCCTG TTGGATGATACTTATTTATTCCCTTTCTGCTCCCTGAGTGTACCCTGTGGGCAGGGCTGTGTCCCCTGTG CTTTTCTACTAAAGGCCTCACAAGATGAGAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:1366538 using vector (pT7T3D-PacI) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:1366538 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14489 same embryo
	EMAGE:14487 same embryo
	EMAGE:14486 same embryo
	EMAGE:14490 same embryo
	EMAGE:14485 same embryo
	EurExpress:euxassay_005340 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source