EMAGE mouse gene expression spatial database

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EMAGE:14541

Rpp14 ribonuclease P 14 subunit (human) ( MGI:1914303)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005422. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005422_01	euxassay_005422_02	euxassay_005422_03	euxassay_005422_04

euxassay_005422_05	euxassay_005422_06	euxassay_005422_07	euxassay_005422_08	euxassay_005422_09

euxassay_005422_10	euxassay_005422_11	euxassay_005422_12	euxassay_005422_13	euxassay_005422_14

euxassay_005422_15	euxassay_005422_16	euxassay_005422_17	euxassay_005422_18	euxassay_005422_19

euxassay_005422_20	euxassay_005422_21	euxassay_005422_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14541_wholemount_strong.wlz

14541_wholemount_moderate.wlz

14541_wholemount_weak.wlz

14541_wholemount_possible.wlz

14541_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14541_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8736
Entity Detected:	Rpp14, ribonuclease P 14 subunit (human) ( MGI:1914303)
Sequence:	sense strand is shown >T8736 CCGAACTTGGGCTGCTGCGTGGGAACTCCGACCCATGCCGAATTCTAGCGGCGTGCAAGAGTCAGGAACG CGGTTATTCCAGTGATCCTCTCCGTCCTGGGCCGAGGCGGTTTAGCCTCGCGGGGCAGGTGTCCATGTTG GAAGATGCCAGCGACTGCATATGAAAGAGTAGTTTACAAGAGCCCCTCAGAGTATCACTACATGAAAGTC TGTCTAGAGTTTCAAGAACATGGGGTTGGACTGAACGTGGCACAGTTCAAACAGCTGCTCGTCTCAGCCC TGAGAGACCTGTTTGGGGAGGTCGGTGCCGCTCTGCCTGTGGATGTTCTCACCTACGATGAGAAGACACT GTCGGCCATCTTGAGAATATGCAGCAGTGGTCTGGTCAAATTATGGAGTTCTTTGACCTTGTTTGGAGCC TATAAAAGCAAGAAGTGTGCCTTCAGAGTGATTCAGGTGTCTCCCTTTCTCCTTGCATTATCTGGCAATA GCAGGGAACAAGTATTGGACTGAATACTCTTACAGGTCCAGATACTTCCACTCTCCACAGTGTCCTTAGG TGTCTACATTATTTGAAAACAGACGAAGGCTAAAGCCCCTGTTGGTGACATTTGGAGATACTGAAGATGT GGCGTGGGCTTCAGAGGAGACTGTGGCAACATCATGTGGTACCAACAGGGCAGTGTTTTCAGTATGTGCA CACGAAAGTTGGAGACCGAGCGGAGCTCAGCAGAGCCTTCACACAGCAGGATGTAGCTACCTTCTCAGAG TTAACGGGAGATGCCAATCCTTTGCATTTAAGTGAAGATTTTGCAAAACACTCCAAGTTTGGAAAGACAA TTGTTCACGGAGTTTTGATAAATGGACTTATTTCAGCTCTCCTGGGCACCAAAATGCCAGGGCCAGGCTG TGTGTTTCTTTCCCAGGAAATTAAATTTCCTGCTCCTCTATATGTTGGAGAGGTGGTCTTAGCATCTGCA GAAGTGAAAAGGCTGAAGCAGTCTGTTGCTGTTGTTGCAGTGTCCTGTTGTGTAATAGAAAGTAAGAAGA CAGTTATGGAAGGCTGGGTTAAAGTTATGGTCCCGGAAGCTCCTAGATCCTGAAATACAAATTTAAAGTT ATACATTTAAAAACCTGTGCTCTTATTATCAAATGACACCCGGGGAGGTGGGCTGCTGCTCTTCCTGGAG GAATGCTGGTATATCCAGAAGCTGTGTGTGGAAGGGGAGACTTCCAGATTGGTGCATGGTTTGTCCAGAC TTAAATATGTGCAAGATCTCATTGTCTATCAAGGTTTGGATTTTTTTTTTCCAATTAAAAAAACAATTGA GAGGGGCTGGAGAGATGGCTTATCAGTTAGGATCACTGGCTGCTCTTCCAGAGTATCTGGGTTCAATTCC TAATACCCACATAGCTGCTCACAACTGTATGTAACTTCAGTCTCAGGTGATCCAACACCATCTTCTGGCC TGTGCCGAAACTACCTGCACATGGTACACAGACATACATGCATACGTGCAAGCAAAATACAAATACAATA ATAGTAAGATAAAAATCTTTTTAAATTGGGAAGACTATCTTCATGTTTTACTTTCAGAATTTGAAACAGT TATATTTGTATTTGATAACGTCTACGTCAGTGTTGAGGGATTTTCTTGCTGGATTTATACCACCATGTAT ACCAAGTGCAGCAATGCAAGTATAACTGCAGCAATGCAAGTAGAGCCATCTCTGAACACCTAATTCTGAG ACCCTTTCTCAGCGGCTTCTGGGCTAGGCTTATAATTCCTTACACCAAGATGTAATCTTTTGTTGCATGT GGTGCCACTGGCCTTTAGTTCCCACTTAGGAGGCAGAGGCAGGCAGAATTTAAAGCTAACCTGTGTGCGT AGCAAGTTCTAGAACAGCCAGGGCTACATAAAGAGACCCTGTCAAACCAATAAAAACAAAAAATGACATT TAATTCAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6404792 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6404792 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14542 same embryo
	EMAGE:14539 same embryo
	EMAGE:14538 same embryo
	EMAGE:14543 same embryo
	EMAGE:14540 same embryo
	EurExpress:euxassay_005422 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source