EMAGE mouse gene expression spatial database

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EMAGE:14570

Zdhhc21 zinc finger, DHHC domain containing 21 ( MGI:1915518)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005443. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005443_01	euxassay_005443_02	euxassay_005443_03	euxassay_005443_04

euxassay_005443_05	euxassay_005443_06	euxassay_005443_07	euxassay_005443_08	euxassay_005443_09

euxassay_005443_10	euxassay_005443_11	euxassay_005443_12	euxassay_005443_13	euxassay_005443_14

euxassay_005443_15	euxassay_005443_16	euxassay_005443_17	euxassay_005443_18	euxassay_005443_19

euxassay_005443_20	euxassay_005443_21	euxassay_005443_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14570_wholemount_strong.wlz

14570_wholemount_moderate.wlz

14570_wholemount_weak.wlz

14570_wholemount_possible.wlz

14570_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14570_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8784
Entity Detected:	Zdhhc21, zinc finger, DHHC domain containing 21 ( MGI:1915518)
Sequence:	sense strand is shown >T8784 GCGGCGGAGCGGGCGGCGGTACTCACAGAGAACTGGTATTGAAGGGAGGCCCATACGATGGAATATGAAA TATAAAGAAATAAATAAAAGAATGACTCAAGAAGATAGCACTTCTAAATGCAAGTGCCAGAAAAATCACA GTTCATCTCAGTGAATTTGCAAGTGGATTGTCTTGCCTGTGGGCGGCAGGCGGGTACAGGACTGCAGAAT GGGTCTTCGGATTCACTTTGTTGTTGACCCGCATGGTTGGTGCTGCATGGGCTTGATTGTCTTTGTCTGG TTATACAATATTGTTATAATTCCCAAAATTGTCCTCTTTCCTCACTATGAAGAAGGACATATTCCTGGCA TTTTAATAATAATATTCTATGGCATTTCCATATTTTGCCTGGTTGCCTTAGTGAGGGCCTCCTTAACTGA TCCTGGAAGACTCCCTGAAAATCCCAAGATCCCACATGCAGAGCGGGAGCTGTGGGAGCTCTGCAACAAG TGCAACCTGATGAGGCCGAAGCGCTCCCACCACTGCAGCCGCTGCGGCCACTGCGTCAGGAGGATGGACC ATCACTGTCCTTGGATAAACAATTGTGTGGGTGAAGACAACCATTGGCTCTTTCTGCAGTTGTGTTTCTA CACTGAGCTGCTTACTTGCTACGCACTGATGTTTTCTTTCTGCCATTATTATTATTTTCTTCCACTAAAA AAGCGTAACTTGGATCTCTTTGTTGTTCGCCATGAGCTGGCTATCATGAGACTAGCTGCCTTCATGGGCA TCACTATGTTAGTTGGAATAACTGGACTCTTCTATACACAACTGATTGGCATCATCACAGATACAACATC TATTGAAAAAATGTCAAATTGTTGTGAAGAAATATCGCGGCCCCGAAAGCCATGGCAGCAGACCTTCTCA GAAGTTTTTGGCACTCGTTGGAAGATCCTGTGGTTCATTCCTTTCAGGCAGCGGCAACCACTGCGAGTTC CCTACCACTTTGCCAATCACGTCTAAACAGATGGATGGTGGGCACAGATGGGTCCTCCATGCTGGAAATG CGTTACAGGTTTTATGAGAATAGAACTATGACAGTCTTCAAGTCAATTAAAATCCACCTACCAATCTTAG GCATCATAATGTGCCCCAGGCTTCATTTTAATAGTGATCTTGATCCTGTTGTGGGACTAATACAAGATCT ATATTTAAGTTTTAAAAGCATGTTTACTTTTAAATTAGCCTTCCACCAGGGTTTCTTTAAATGCGTTTGT TACTAAACTCAGAAGGCAGTGATTGGGATGAAACAATTGTACATACATTTTCTTCAGTACTCAAAGTTTT TTTAAATTTTAATTTATAGGAAATTTGACATGTTTTATGTTTACCTTTAAGCAGTCCTAACCAAATCTGA ATTTAATTCTTCAAGTTTACAAAACTGGATACACTTTGTTATGTATAAGTTAAAATGTTACTACTTGCTT TTATATGTGCTGTAGGCTTTTACAGTGCAGTGAGCAACAAGGGGCAGTGCCTGGCTAGGAGCAGCTCTCT GATGCCCTTCTTTCTCCTTCTCAAAACCACAGACGACTCCCCGAGTGTAGTGCACCAACTACTACCTTAC TGTGAGGGAAAAACTATGTTTCCCTTCTGCTGCAATCACTGATTGCTTTTAGAATATTTGCTCCCAGGAG AAATGTGTGACCAGTCAGGTTTATATTCCTGTTTTACCCTAAGATTCTAATGACTGAAGAGGGTAGGAAG TTTGCAATAATTCCAGTTACTTCTTCAAGTGCAGTTATATTAGAAAGCAGATGTTTTAAAGTGCTAAGCT GAATGGTGTGTAACAGTGTAAGGAGGAGAACTGTGCTTTTAAAGTATGGATACTTTTATTTATCTGCCAT TAGATCTCACTGTACACATTGAAGATTTATATATTATAGAATCGATACTGGTCAACTCATATCAAAGTGT AAAAGTGGGTCACTAAATTTATATACCAGTAATCACCCAAGTTGAAATTGAATTTCCACTTGTAATTCGC ATCACTCTAGAGACCTTTTATTAATGTCAGGAAATTACTGTAAAATAGAATGATCTATGGTAGTGATGGT CTCTTAACAAACATGAGCCTTTTGTATACAAAGACATTTTGTATGATTTAATTTTTTTCATTTAGAATAC TGCAATTCCATATGTTTGTGTGCTTAGTAATCTTTTCCCTGAAAAGAGTATTGAACAAATCTACCATTTG TGAACCATCTACATACTTTTCTTATAAGTAGTTGTAAAGTAAGGAATCATAGCTGGGAAATACTGTGGCT AGTTCTTTCAGATACTGTAAATGTCTTATAAAATATTCTGAATAGAATAATCTCTTTGCAGAAATATTAA TACCATATTTTCATGTTCTAATTATGTTTATTTAAAAACTTAGAAGTGGCAGATGAAAATATTTATTGGT GCTAAGACATATAGTAAGTTTAATATAAGAAACATTTATAAAGGTTAGGAAACATAAAATTAGTATGTTT TAGTAAGGGCGAAGGGGTTGGGTTTTGTGTAAGCTGATTGACTAACTTGGCCAATGACAGTTACTGAAGG AAGTGGTGTACATAGACTGTTGGGAGGTAGAAGTTGAATGCCTTCAGCTGTATATATTCAGTTCACTCAA GACCTGGAGCATCCAAAGCAGAGCCAAACGAAAGCCCAGCGTGGAGCTTTGTAAACTGCTTTATACTTCT GGAGCAGTAGTAGCTCTGACACAAGGTAACGGGCGTGATCCCTTCCCCATGCTGGAGTTCGTGGGTTTGG TCTGCTTACATGTACCACTTCTGGGCACATTATCTACCCTTTCTCTTTACTCTCCCTGGTCCTGACCCCT CTCTGCAGTTTCCTAACTAGTCATTGTACTTGATGCACTAGTTTGATCTACAGAAATGCTGTTATTGATG TATTTACATAAGAATCTATATTGCAGTATATCTATATATACTTACATAGTATTGTTAAATGTATATGTCT AACCCCCGGAGGGGTTTGTAATCACTCTAAAGATTCCTAGAGAAAAACCTGTTGGCCATTTCCTAGGGAT TAAGCTCCTATGTTCTTTCTGCGCCCTCTTTTGGCTAATTGATGTAATTATCCTGGCTCTTGAGGTTTTT ACTATCCTGTTAGTGGGTTGTATAGCCAATCTGTGAACATGCCAGAGGTATACATGCCAGTGTATACTTA TAGGAACATATGTATAAAAGTGTAAAGTTTTATAAAAATTGAAAATGAATGTGTTATTGTTGGTGATGTT CCTTCTTGGTAAACTGCCTGGCTTCATGTATGTATATTCTTGTACACTTTTTACTGTCAGTCACAAATTT CGATTAGTTTAAGCTGTTTCATGTATTTTACTTTAAACCTTATTTTGGCAACTTATCTTGTTAGCATTAT AAAAACTTACTTAAAATACTATTTTACTACAAAACAAAACATTTATTACAGTTGCTGTGGTAACTGTTAT TCGGTATGTTTTACTATTTTAAAAAGTACATTTAATTTTTTCAATTCAATTTTGGGATTGTCCCCTTCCA CACACACACAAAGGTTCAATTACCTTAAAATAATTAGTAATTGGTTAATAATATTATTTTTCTTTGCTTT TTAAAACGCTTGTCAATGAGAGTTAACCCTGTAGATTATTCAATAGCCTCTTTATTTGTCCCTCTCAGTA ACCATGGGAATAACTACCCATGTGGGGTCTCAGGAAGCGATGGAGCAAGGGCAACCCCTTCATATCTACA GTGCATTGAAGTATTTATAAGCTTCCTGTTTAATTTTAGCAAAATGTGTGTTAAGTGACATTTTTACATT AGCAAAGTCACCTCATGGGATCCTGCAGTTAAAGTGTTAAGACGAGGCTGCAGATACTTGGTAAACAATT AGAATTGGGCACGCAAGGTGGCTCCATACAGAAAGGCATTTGCTGTACACGGACCCACTTAGGTGAAGAA GGGGAAAAAAGCTCCTGGCTTCATAGAGTTGTCCGCTGACCTCCACAAACATGCCTTGTCAGTCACGCCC CTCCCCATCATCATTGTTCATTCATCGCCTCACACACAGCACACACAAACGTTTCTGATATCCAATTGGA TAAGCTTGATGTAGGTAACAAAGGGCTGGGTTCAGAATCTCCTAAGCATTAAACAGTCTACTAAGGAGTC TTTGTTACCAGGCTTCCATTTCAGATTACTAAGCTAGCTGATGTTACATGAAAATAAAAACCTATTTTGT TTTATAATTTATATTTAAAATGTTTTAGTTAACATAAAAGCCATTTAAATTTGTAGCTAACATAAGATCC TGTTTTTCAGAAGACAATTTACTTTTAATAGTAATAAAGTAGGGGCTGATGTGTACCTCAAAAATTATAG TGCACAAGATGCCCAGGATTTAATGCCAAGAACCAGGAAAAGACAAGAAGAGGATGTAGACAGTAGCTAC ACACTAGATGCAGAAGAGCTGAGTTGAACATCTCAGAACGTTTCATTAATGTGCAGAAGTGGTTCCATGA AGGACGGTGGCTCATTCCAGCTATTCTTAATGAGACTTTGGGTTATTTTCCTACAAGCAACTGTTTTGCT CGAGAGATGTATTAGTTCTAAACAGTAATCACAAAGACTAAAATTAAGAACACACTTCCTAACGGGAGAT ACAAATGAATTTAGATGAAAGCCACATAGTTTTTGTGTTAGTTCATGTGTAGTTTGAATGTTCTTACATG TATTGCTAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6406930 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6406930 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14569 same embryo
	EMAGE:14568 same embryo
	EMAGE:14571 same embryo
	EurExpress:euxassay_005443 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source