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EMAGE:14587

Zdhhc19 zinc finger, DHHC domain containing 19 ( MGI:2682948)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:14587 EMAGE:14587
euxassay_005482_01 euxassay_005482_02

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:14587Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
14587_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:14587_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T9868
Entity Detected:Zdhhc19, zinc finger, DHHC domain containing 19 ( MGI:2682948)
Sequence:sense strand is shown

>T9868
ATTAAGGGAAAGACCAGCTAGTATGAAGTCTTATAATTTACCTATACACCCTGCTTGTACTGGGACCTGA
GGATTGAATTCCGATCCATGTACCTGCACAGCAAGCCCTTTGCCCACTGAGCTGTGTACTCTGCCCTGGC
CATGCCTTTCTTAAAGGATGCTGTGACACTTGTGAAGGAACCACAACAGCTGCCCTCCATCCCTCTTTCA
TGGTTCCCCTCGAGCGTGTTTGCTGCCTTCAATGTAACGCTGCTGCTGTTTTTGAGTGGCCTTTTCTTCG
GATTCCCTTGTAGGTGGCTGGTTCAGAACGGGGAGTGGGCCTTTCCTGCCATCACAGGACCACTCTTCAT
CCTCACCTTCTTCAGTCTCGTCTCGCTCAACTTCTCAGACCCTGGTATCTTACATCGAGGCTCCACCAAA
GAGGACCCCATGACGGTGCATGTTGTTCGAGTGAACCAAAGGGCTTTCCGCCTGGAATGGTGCCCCAAGT
GCCTCTTCCATCGCCCACCCCGAACCTACCACTGCCCGTGGTGCAACATTTGCGTGGAGGACTTCGACCA
CCATTGCAAGTGGGTCAATAACTGCATCGGTCACCGCAACTTCCGCCTCTTCATGCTGCTGGTCCTGTCA
CTCTGTCTCTACTCGGGAGCCCTGCTGGTCACCTGCCTGACATTCCTATTTCGCACAAGGCATCTGCCCT
TCTCCCTGGACAAGGGGATGGCCATCCTGGTGGCTGTGCCCGCTGCGGGCTTTTTGATTCCGCTCTTCCT
GCTGCTGCTGATCCAGGCCCTATCTGTGAGCAGGGCGGAGAGCTCCTACGAGAGCAAGTGCAGGTACCAT
CCGGAATACAACCCTTTTGACCAGGGCTTTGCCAAGAACTGGTATCTGGCAATGTTTGCACCACTGGGTC
CCAATTACATGTCTGAAGTTGTCTGTCTGCAAAGACCAGTGGGGACAGCATGGATCCAAGAGAAGACAAA
GCCCTCACCACCACGTCGTCCTAAACACTGTAGGCCAGGTCCTCCGGGGCCCCAGCACCAACCTCGGCGC
GTTCCAGGGAAGGGGCCCCCAGGGAGTGGAGAGGCTGCAGCTCTCCAAGAGATGCGAAGGCTGCCTGCAT
CTGTGGAAAAGTCCCCAGGAGGTCCACGCCAACCCACGGCTGAACCTGCCGCTGGAGACCCCTAGATGAG
ACTCTCCCCAGTGCACACAGAGCCAGGACCACCTGAACTTCCTTCCGCCTCTGAGTCTGAATCCTTGTGG
TCCACTTGGGACCTACCCATCCTCATTAAATATTAATTGTTGATTTCAGAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:6773873 using vector (pDNR-LIB) specific primers. Forward Primer - name:M13 forward (-21), sequence:TGTAAAACGACGGCCAGT; Reverse Primer - name:T3-pDNR-LIB rv, sequence:CAAATTAACCCTCACTAAAGGGTGTTCACTTACCTACTGGGC. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6773873 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:14584 same embryo
 EMAGE:14586 same embryo
 EMAGE:14588 same embryo
 EMAGE:14585 same embryo
 EurExpress:euxassay_005482 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS