EMAGE mouse gene expression spatial database

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EMAGE:14646

Map1lc3b microtubule-associated protein 1 light chain 3 beta ( MGI:1914693)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005494. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005494_01	euxassay_005494_02	euxassay_005494_03	euxassay_005494_04

euxassay_005494_05	euxassay_005494_06	euxassay_005494_07	euxassay_005494_08	euxassay_005494_09

euxassay_005494_10	euxassay_005494_11	euxassay_005494_12	euxassay_005494_13	euxassay_005494_14

euxassay_005494_15	euxassay_005494_16	euxassay_005494_17	euxassay_005494_18	euxassay_005494_19

euxassay_005494_20	euxassay_005494_21	euxassay_005494_22	euxassay_005494_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14646_wholemount_strong.wlz

14646_wholemount_moderate.wlz

14646_wholemount_weak.wlz

14646_wholemount_possible.wlz

14646_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14646_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9613
Entity Detected:	Map1lc3b, microtubule-associated protein 1 light chain 3 beta ( MGI:1914693)
Sequence:	sense strand is shown >T9613 CACACAAGGGAAGTGATCGTCGCCGGAGTCAGATCGTCTGGCTCGGGACCCCCGCGCGCCATGCCGTCCG AGAAGACCTTCAAGCAGCGCCGGAGCTTTGAACAAAGAGTGGAAGATGTCCGGCTCATCCGGGAGCAGCA CCCCACCAAGATCCCAGTGATTATAGAGCGATACAAGGGGGAGAAGCAGCTGCCCGTCCTGGACAAGACC AAGTTCCTGGTGCCTGACCACGTGAACATGAGCGAGCTCATCAAGATAATCAGACGGCGCTTGCAGCTCA ATGCTAACCAAGCCTTCTTCCTCCTGGTGAATGGGCACAGCATGGTGAGTGTGTCCACTCCCATCTCCGA AGTGTACGAGAGTGAGAGAGATGAAGACGGCTTCCTGTACATGGTTTATGCCTCGCAGGAGACATTCGGG ACAGCAATGGCTGTGTAAGACTCCAACAAAGCCAATGGTTGTTAAGCCCTTACCAAGGCAAAAAGGGATG TTACCAGCGGACGCTGGACGGCTCACCACCCACAGATGAGAACGTAGGCACCCACATAGGGTATTAGGAA CTGTTCATCAGCCAGAAACTGAGCTCCATGCAAGTGCACTCAGCTTGGAAACTCGTCTAAACTAGGCTAT TTTGTGTTCAAACTTTAGAAGTTAAAAATAAAATACTTTTTGCATCCTAAGTTGCCAATAAAAAGATCAA GTTATTTTGACATTTTTCTCCCTAGTAGGGACTTAAAACAGAAGAGCAGTGTCAGGGGCAGTCTCTTCCT GGGACCTGCTGCCTTCTCTAAAAGGGCTCCCACTCAGCAGAGAAGCCCCTCTGAAGGCCCAGGTTGCCTA GCAGAGGCCCCCAGAGGGCCCTGGCTTTGCCCTAAGCAGGTCTGGTGTTGTAGGATATAGCTCTAAGCCG GGTCCTGGTCTTGACCCCAGCCACACCCTTTCACTCAGCAGTGCAGGGACATGCAGCTCGCTGCTGTCTA GATGTTACCATACGCCCTTCTGCTGAGGCCAGGTCTCCGCTTGTCCAGGGGTTGCTGACCCAGCTTAAGC GACTGGAGAGCTGTTTCTCTCCTGGTTTGAATTCTGTCTGGTCCATGCCTGCTTCAGGGAGAGCAAGCGC TGGCCTTAGGATGTGGTTGTCAAGTGGTAGACTGCAAGTCCAATGCTCCAGACTTGGCTCACAGCTCTTT GTTGGTGTGTAACTGTCTCTGTAAGGGTGGTTCTGACAGCTGTTGGGATTTCCCCATTTCATGTCAGTAC ATACATGTCAGCTGTGAGGACAACAGCAACAGAAGACATGCTTTTGATAGACATGCCACTTCAGCTAAAG CTAGCCTCTGCTTCCTGCATGTGGCCCGCTGTGACACCAGGCCATCCCTGGTCACTCACTCACTCGTGGT CTAAGGATCTCAGAGCTGCCTGCTGTCATGGACTGAAGCCAGCATAGCCACCTCCCACCTAGCTCCCCAC AGGCACCCACCTTCCCTGCATGCAGCTGTCTTTGCTAACCCTCAATGTTATTTTACACTGTAAATTCTCA CTGCTCTGTCTTGTGTAGGTTGTATACGTCATTATGTCCCTGGTTTTTATAACTATGGTGCGATCAGTAA GGATTCCTGTAATACTGCTTTAAAGATGCTGCTCAGAGGGCGGGCGGCCTCCATTATAGGACACCTGTAC ACTCTGATGCACTAATAAAGGCACAACGAACCTGAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30634082 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30634082 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14643 same embryo
	EMAGE:14642 same embryo
	EMAGE:14645 same embryo
	EMAGE:14647 same embryo
	EMAGE:14644 same embryo
	EurExpress:euxassay_005494 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source