EMAGE mouse gene expression spatial database

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EMAGE:14677

3830406C13Rik RIKEN cDNA 3830406C13 gene ( MGI:1917937)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005605. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005605_01	euxassay_005605_02	euxassay_005605_03	euxassay_005605_04

euxassay_005605_05	euxassay_005605_06	euxassay_005605_07	euxassay_005605_08	euxassay_005605_09

euxassay_005605_10	euxassay_005605_11	euxassay_005605_12	euxassay_005605_13	euxassay_005605_14

euxassay_005605_15	euxassay_005605_16	euxassay_005605_17	euxassay_005605_18	euxassay_005605_19

euxassay_005605_20	euxassay_005605_21	euxassay_005605_22	euxassay_005605_23	euxassay_005605_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14677_wholemount_strong.wlz

14677_wholemount_moderate.wlz

14677_wholemount_weak.wlz

14677_wholemount_possible.wlz

14677_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14677_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4980
Entity Detected:	3830406C13Rik, RIKEN cDNA 3830406C13 gene ( MGI:1917937)
Sequence:	sense strand is shown >T4980 GCGTCGTCCATGGCGCGTTCCCGGAAGAGCTCAGAGAAAACTTATAGCCCAGCTTTGATTAAAGATGGCT TCCTTGTTTACTCAAGAAATTCACCTTTCTAAAAGACATGAAGAAATATTATCACAAAGATTAATGTTAC TTCAGAAAATGAAAAACAAATTTGGAGATGAAAACACAGAAAGGGCATCTCTCCTCCAAGCCACTGAGAC TGCTTCTAGAAGAAACCTCCGACTCTTGAAGGACATAGATGCTGCAGAGGAGGCTTTTCAGACCAAGCTT ATCCCACACCCACAGCCTTCGATGCTTTCTCTTGAGACTCGTTACTGGGCATCAGTAGAAGAACATATCC CCAAGTGGGAACTGTTTCTTTTAGGAAGAGCACCATATCCAATTGGTGCTGAAAATCAAAATGAGGTACC ATTCGTTCAAACCGAGGCAAAGTTGTAACTTATTCATGTGCTCTTTGAAACTTTTTTTTTTTATAAAACT ATGTAAATCCATGGCGGGGACTTAGGGAAGAAGTTTCATTTTGTTATTGTTTTCTATTGTCTCATTGTTT CCTCAGCAGCAACGTCAGTTTGAGAGCCCTCTGAAAGGTCTCAGGTTAGATCCTTGCACAGTCATAGACT TGCCAAGAAAAGTGTAAAAATTAATGGTAAGACATAATGTTTGTTAAATATGGTATTAAACCAGTGTTAT GTGTGCTTAAAACCCACACCCTCATTCCCAACTAAACAGGTTGGCTGCAGGTATCCTAAAGTTGCGATTT TTCATCATTCACTTTGGGAACAGAAGCAGGAACAAGGTGCTGCCTTCCTTACATGAAAGTCTGCCACGCT TAGCTGCTCTTGTTCAATACTAGTTATCAGAAAATAGATTCCTGGGGTTGTGTGCTTCCTACAGACTCCT CCAAGTCCTTAACTCGAAAGCATTCAATATGTGGCCAGTTTGGGACAGCGCGAGTGGTTTTGAAAAAAAA AAATCTATGAGAGCTGGGAATGGCTACTGAATTCTGACAGGCTTATTTGAAGATTACCAACGTTGTGAAA ACAAATACCCCTTCCTAAATGTCATCTTTCAACTCGTCCTCCCCTCCTTTCTATTATTATCTCTAAAACT TAAGAATAAAACCCAGAAAGCTGTGGATCACGGCCCTCTTGAGTCTCCAGTTCCTTCCTGGCTGCCGAGC ACTGGGGAGAAGACAGAGCGAGCTTTCCTAGCTGTGGAGACAGCTTCTAGTGAGGGGGAGCCTACACTGT TCCACAGTGGGGATTCCAGTCACAAATGTGCCTTCACTGTGGTAAAGGTTCTTCTTTTGTTTTTGTTTTT TGTTTTTATTGCCTTTAAGATAAAAATCTCACCCTGTAGACCAACCTGGCCTTGAAGTCACAGCCATTCT CCTGTTTTAGCCTCTGATAACTGAGATTACAGGTATGAGCCCCAATATTTAGGTCATTTGTATTTTTAAA AGAAAACAACAGAAAAAGTTAAAATGCATATAAGCCCTCACTACAACAAAAACTAGTAAATCATCCATTG TGTAACATGTTCCTGTTCACTGCCATCTTGGAAATGTCCTGTTGAGTGGGTGTTGGTGTGGGTAACAATA ACATTGTTTTCATAATATGTGCTGGCAGATTAGCACACTTCTCTGCAGTGTTAACAGTTGTAATTAAGGA ATAAACAGCACAAGGTGTCAGTCCGATGACAATTTCATCTATAGTGTCACAATACAAAATGTCACAAAAT ATTAGAGCTGTTACATACATAGCTATAAAAAGTATCGGCTTAAGAGCTGAAGGCATCTTTAAGAGAGGAG ACAGAGAGGAGGCAATGGAACACTGACTTAGCGGCCAGAACAATTTTATACATTCTCAAGACTGTCATGA AAACAAATTGATAATTTTTTCTGTTACATTACAGTTTGGGCTCCAGCCATGCAGTTGATTTAGCAGGGAA ATGCAGGTTTCTCAGCAACTCCAAATCAGTAAACAGATTTAGACGTAGACAGGAACACGAGGAAGGGCTC TTTTTAAACATCCAGTCTCCCAGCATATGCACATAAAAAGGTGAATGAAGCAACGTGAAACGTACTTCTC TTCAGTCAAGTGAAAAAGGCTAAACATTTGTTAGCTGGTAAAATATTGTGTCAAGTAAGCCGGAAGATTC CAAAATTAAACAGTGGTTGATATGTTAACGTCCTCAAATATTTGAGTGCACACTTCCTTTGAGATCTGTA AATTAAATCACCAGTAGGTTGAATTACAATACAAAATGAATGGCATTGTAAAAAAAAAAAAAAAAAAAAA AAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5137611 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5137611 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14679 same embryo
	EMAGE:14680 same embryo
	EMAGE:14675 same embryo
	EMAGE:14678 same embryo
	EMAGE:14676 same embryo
	EurExpress:euxassay_005605 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source