EMAGE mouse gene expression spatial database

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EMAGE:14693

Chtf18 CTF18, chromosome transmission fidelity factor 18 homolog (S. cerevisiae) ( MGI:2384887)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005618. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005618_01	euxassay_005618_02	euxassay_005618_03	euxassay_005618_04

euxassay_005618_05	euxassay_005618_06	euxassay_005618_07	euxassay_005618_08	euxassay_005618_09

euxassay_005618_10	euxassay_005618_11	euxassay_005618_12	euxassay_005618_13	euxassay_005618_14

euxassay_005618_15	euxassay_005618_16	euxassay_005618_17	euxassay_005618_18	euxassay_005618_19

euxassay_005618_20	euxassay_005618_21	euxassay_005618_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14693_wholemount_strong.wlz

14693_wholemount_moderate.wlz

14693_wholemount_weak.wlz

14693_wholemount_possible.wlz

14693_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14693_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4988
Entity Detected:	Chtf18, CTF18, chromosome transmission fidelity factor 18 homolog (S. cerevisiae) ( MGI:2384887)
Sequence:	sense strand is shown >T4988 GCGCGGTGGCGGCGGCGCGGGAAGTTCTGAGGGACTCTGAGCTGTGGGAGTTGTGGTCTGGCTCCATGGA AGACTACGAGGAAGATCTGTATGGTGTGGAGGATGATTTCCAAAACCAGTTCGCAGCTGAGCTCGAAGTG CTGGCTGAGCTGGAAGGAACACGGGACCAGGCTCCTCCTGGGACCCTCCAAACTCCAGCGAGCCGCCCTC CGCTAACCTTCGAAGAGGCCATCGCTGGTGGGGACACTGTCCCGCGTCCCTGCCCTGCAGGGTCGCCAGG AAATGTCAACCGCAATACTAGAAAGAATGTTCGTAGGGACCAGCCCGCGCCCTCCAGCCCCATGGTCAAA CGACCCAGGTTGGACGTGGTGAAGAAGCTGAACTTTGAGCCAGATATGGAGGAGCTCTTATATCCAGATT CCCCTCCTGGGGACATTACCCCTCCACCGAGTCCAGAGGTCTTCCCTGAGATGTTGGATGCTGGGTATTC AGATGCTAATGCTGACAAAGACCTCATGCAGACATTGCCAAGTCCCCGAAATCGCAATCCTGTCCTGAGA CGGCCTCCTATCTTAGAAGATTATATCAATGTGACATCCACAAGTGGAGAACGGGCCTTTTTGGTGCTTC GGGCTGACCTCATAGGCCCTGGGGTGCAGAACCCTCTTCTAGATGTCCACTGGCGAGGCTGTGGCCAACT GGACCTGCTGGGTGTCCCCTTCGCCTCCCTGAAGGAGCAAGTTGATAGCAAGAGACGGCAACAACTGCTG GAGGATGCCCAACAGCTTTCAGACACTCTACACAGCCTCAGGTCTGAAGGAGAGGAGGCTGTGCTTGAAG GGCCCCCTGCGGAGGAGCCAGCCCCTGGCCAGAACACCGCTCAGCACTGCCTCTGGGTGGATGAGTTTGC ACCTCAGCATTACACGGAACTGCTCAGTGACGATTTTACCAACCGCTGCCTACTCAAGTGGCTGAAGCTA TGGGACCTTGTGGTGTTTGGCCGTGAGAGACCTGCCCGGAAGCCCAGACCTGGTGTAGAGACCACCCGGG TTGGGAAGGAGGCTACAGCCCCTGGCAAGTGGAAAAGCCATGAGCAGGCACTGGAGGAGATGCTAGAAGC TGAACTGGACCCTAGCCAGAGGCCTCGGCAGAAGGTGGCACTGTTGTGTGGCCCTCCAGGACTAGGCAAG ACCACGCTGGCACATGTGGTCGCAAGGCATGCAGGGTATTGCGTGGTAGAGATGAATGCAAGTGATGACC GTAGCCCGGAGGCCTTCCGTACACGCATTGAAGCAGCTACGCAAATGGAGTCGGTTCTGGGTGTGGGTGG GAGGCCCAACTGCCTGGTTATTGATGAGATCGACGGGGCCCCCACGGCGGCCATCAACGTTCTTTTGGGT ATCCTGAATCGCAAGGGTCCGCAGGAGGCCGACCAAGGAGGCACAGCTGTGGCCGCAGGGGGGCGGCGGC GCAGGGCCGAGGGGGGTCTCCTAACCAGGCCTATCATCTGCATCTGCAATGATCAGTTCACACCTTCTCT GAGGCAGCTGAAGCAGCAGGCTCTTCTGCTCCACGTCCCCCCAACTCTGCCCTCCAGGCTGGTGCAGCGG CTCCAGGAGATCTCCCTGCAGCATGGCATGCGCTCTGACCCGGGTGCACTGGTTGCCCTCTGTGAGAAGA CCGACAATGACATCCGTGCCTGCATCAACACCCTGCAGTTTCTGTATGGGAGGGGCCGGCGGGAGCTGAG TGTGAAGGCTGTGCAGACCACTCATGTCGGCCTGAAGGACCAGCGCAAGGGGCTATTCTCTGTGTGGCAA GAGGTCTTCCAGTTGCCCAGGACTCAAAGGAGAATCGTGGGCCAGGACCTGATGCTGCCAGCCCACGCTC TCCTGCTCAGCAATGGTGACAAGGGCTCTCTGACCCTGGCCTCCCAGCGCTTTTACCACATCCTGCGTGT GACCACCTCTGCGGGCGAGCATGAGAAGGTTGTCCAGGGCCTGTTTGACAACTTTCTACGCCTTCGGCTT CGGGACTCCAGCTTAAGCACCGTGTGCTGTGCCCTCGACTGGCTGGCCTTTGATGACCTGCTGGAGCAGG CTGCCCACCGTGGCCAGAGCTTCCAGTTGCTGTGCTACTTGCCCTTCGTGCCTGCGGCCTTCCACGTGCT CTTTGCCTCCAGCCATGTGCCACGAATCACTTTCCCCAGCAGCCAGCAGGAGGCCCAGACTCGGATGAGC CAGACAAGGAACCACATACAGACACTGGTGTCAGGTATGGCACCGACCACGCGTAGCCGGGCCACACCAC AGGCCCTTGTTCTAGATACTCTCTGCCTGCTCCTGGATGTCCTCGCACCCAAGCTGCGCCCCGTGAGCAC ACAGCTGTACAGTGCCCATGAGAAGCAGCAGTTGTCCTGCCTTGTGGGTACCATGCTTGCCTACAGTCTC ACCTACCACCAGGAGCGCACACCTGATGGGCAGTACCTCTACAAGCTGGAGCCGAATGTGGAAGAGGTCT GCCGCTTCCCTGAACTACCTGCCCGAAAGCCCCTCACCTACCAGGCTAAGCAGCTTATTGCTCGGGAGAT TGAAATGGAGAAGATGCGCAGGGCAGAGGCTTTGGCCTGGGCTCGAAGTGGCCCCCAGGTGGACCAGGGC TCCTCAGGGCCTGCAAGTCTGTGGACAGACAGTGGGGAAAAGGAGACAAGGCAGCCTGCCCCACGTAACC ATGAGCAGCGCCTAGAACACATCATGAAGAGAGCAACCGTGCAGGAGCAGCCTGAGAGGGACTTCTTTGG ACGTGTGGTCATCAGGAAAGTGGCAGTCCCGAGCAGAGAGGTTGAGGCCCCCCAGAAGGACGCGGACGAG TGGCGCATGGGTGTGGCAGTGGGCAGGAGTGAGGTGTGGTTCCGGTTCAACGAGGGCGTCTCAAATGCTG TTCGGCGCAGCCTGTACATCAGGGACCTGCTGTAGCCCCACTGCCCCAGGACATCAGATCGCACTGGCTC CCACAGGGAATGGACTAAAGCAGAGACACCCGGAGAAGTCTATTTATAAAGTCACACCTTACAGAGAGTG TGGGTCAGGCTCAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5149957 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5149957 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14694 same embryo
	EMAGE:14695 same embryo
	EMAGE:14692 same embryo
	EMAGE:14697 same embryo
	EMAGE:14696 same embryo
	EurExpress:euxassay_005618 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source