EMAGE mouse gene expression spatial database

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EMAGE:14713

Rps6kb1 ribosomal protein S6 kinase, polypeptide 1 ( MGI:1270849)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005666. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005666_01	euxassay_005666_02	euxassay_005666_03	euxassay_005666_04

euxassay_005666_05	euxassay_005666_06	euxassay_005666_07	euxassay_005666_08	euxassay_005666_09

euxassay_005666_10	euxassay_005666_11	euxassay_005666_12	euxassay_005666_13	euxassay_005666_14

euxassay_005666_15	euxassay_005666_16	euxassay_005666_17	euxassay_005666_18	euxassay_005666_19

euxassay_005666_20	euxassay_005666_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14713_wholemount_strong.wlz

14713_wholemount_moderate.wlz

14713_wholemount_weak.wlz

14713_wholemount_possible.wlz

14713_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14713_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T796
Entity Detected:	Rps6kb1, ribosomal protein S6 kinase, polypeptide 1 ( MGI:1270849)
Sequence:	sense strand is shown >T796 TCCTCGAGCCTGTTGGCCTACTGGAGACGCATTGAGCTTAAGCAGCCGGTGATGGCGGCAGCAGCCGTGG AGTCTGCGGCGGGTCCGGGCCCATGAGGCGACGACGGAGGCGGGACGGCTTTTACCTAGCGCCTGACTTC CGACACAGGGAAGCTGAGGACATGGCAGGAGTGTTTGACATAGACCTGGACCAGCCAGAAGATGCAGGCT CTGAGGATGAGCTGGAGGAGGGGGGTCAGTTAAATGAAAGCATGGACCATGGGGGAGTTGGACCATATGA ACTTGGCATGGAACATTGTGAGAAATTTGAAATCTCAGAAACTAGTGTGAACAGAGGGCCAGAAAAAATC AGACCAGAATGTTTTGAGCTACTTCGGGTACTTGGTAAAGGGGGCTATGGAAAGGTTTTTCAAGTACGAA AAGTAACAGGAGCAAATACTGGGAAGATATTTGCCATGAAGGTGCTTAAAAAGGCAATGATAGTGAGGAA TGCTAAGGACACGGCCCACACGAAAGCAGAGCGGAACATTCTGGAGGAAGTGAAAC
Notes:	The probe template was PCR amplified from IMAGE:1920943 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1920943 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14715 same embryo
	EMAGE:14711 same embryo
	EMAGE:14712 same embryo
	EMAGE:14714 same embryo
	EurExpress:euxassay_005666 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source