EMAGE mouse gene expression spatial database

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EMAGE:14718

Zfp810 zinc finger protein 810 ( MGI:2384563)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005671. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005671_01	euxassay_005671_02	euxassay_005671_03	euxassay_005671_04

euxassay_005671_05	euxassay_005671_06	euxassay_005671_07	euxassay_005671_08	euxassay_005671_09

euxassay_005671_10	euxassay_005671_11	euxassay_005671_12	euxassay_005671_13	euxassay_005671_14

euxassay_005671_15	euxassay_005671_16	euxassay_005671_17	euxassay_005671_18	euxassay_005671_19

euxassay_005671_20	euxassay_005671_21	euxassay_005671_22	euxassay_005671_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14718_wholemount_strong.wlz

14718_wholemount_moderate.wlz

14718_wholemount_weak.wlz

14718_wholemount_possible.wlz

14718_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14718_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6159
Entity Detected:	Zfp810, zinc finger protein 810 ( MGI:2384563)
Sequence:	sense strand is shown >T6159 AGCCGGTGTCCCAGGTTCGGGGGACTCTGTCGGTACCGGCACGGGTCTGCGCCGCGCAGCGAGGACGCGG GGTGTCTCGACTCCGCCATGATGTAGACTCGAGTTTTCCTGGGAAATCCTCATGACATACTACTTCCAGA AGGCCCAAGGGAGGAACTCTATTCCCTGGAAGCTGGAAATGGGCACTGCATGACCAAACCAGAGCTCATC TTTGAGTGGGAGCTTCGATTTGGACCATGGACCACAGCAGAAGCCTCAGTTGGATGCGTGTCCGGATCAT CTTGATCAAGAGGCACTAATGCCATCTCTGTTCCCGCTGACATGCTGCTGCCTTGAGAACCATAGTGAGG ACGCTGGGTGTCCTGGAATCTGGAAATGGTGTTGGTGTCATTTGAGGACGTAGCCGTGGACTTCACCTGG GAGGAGTGGCAGGCCCTGGACGCTGCTCAGAGGACCCTGTACAGGGACGTGATGCTGGAGACTTACAGCA GCCTGGTGTCCTTGGGGCACTGCATGGCCAAACCTGAGCTGATTTTCAACTTAGAACAGGGACTCGGGCC ACGGAGCACAGCAGGAGCCTCAGTCTGGAACCTCTCAGGTGTCAACAAAGTGAGTAGCCTGGTTGATACC ATCCAGGAAAATGATAGCAGACATTTCTGGCAAATTGAAATCAACAGCCATACAGCAAATGAAGAACTGG TTGAAGCAGAACTGAAGATTCATGAGGAAATCCACCGAGGGGCCAAATCCTATGAATGTGAAGTGTGTTT GGAAGCATTTTACCTGAAGCCACAGTATAGCACAAAGCAGAGCTATCACACATGCGAGAATCCATGTGAC TGTAAGAAGTGTAGAGAAGCTTTCTATCCTAAGTCTACCCTCAGTCAGTATCAGAGGCTTGAGAGAGGTG AGAAACCCCATGCGTGCTTAGAGTGTGGGAAATCCTTCTACTGCAAGTCTCACCTCACTGTGCATCAGAG GACCCACACCGGTGAGAAGCCTTATGACTGTAAAGAGTGCAGGAAGGCTTTCTACAGCAAATCCCAGCTC AATGTGCATCTGAGAATTCACACCGGTGAGAAGCCTTATGAATGTAAAGACTGCAGGAAGGCTTTCTACC GCAATTCTGACCTCACTGTACATCAGAGAACTCATACAGGTGAGAAGCCCTATGAATGTAAAGTCTGCAG GAAAGCTTTCTACTGCAATTCACAGCTCACTGTACATCACAGGACTCATACAGGTGAGAAGCCTTATGAG TGTCAAGTATGCAATAAAGCTTTCTACTGCAAGTCACAACTCGCTGTACATCACAGGACTCATACGGGCG AGAAGCCTTATCAATGTAAAGAATGCAGGAAGGCATTCCAGTGTAGGTCAGACCTCACTCGACATCAGAG AACTCATACTGGTGAGAGGCCATATGAATGTCCCGAGTGCAGGAAAGCTTTCTACCGCAAGTCAGACCTC ACAGTACATCAGAGAACACATACAGGGGAGAAGCCCTACGAATGTAAAGAGTGCAGGAAAGCTTTCTACT GTAATTCACAACTTACTGTACATCAAAGACAACACACAGGCGAGAAGCCTTATGAATGTAAAGACTGCGG GAAAGCTTTTCAGTGCAAATACGAACTCACTCGACATCAGAGAACACACACAGGCGAGAAGCCTTATGGA TGTAGAGAGTGCAAGAAAGCTTTTTACACCAAGTCAGATCTCACTCGGCATCTAAAGACTCACGCAGGGG ACAAACCCTGAAGATATAGAGCAGGGTAATACTTAATAAGTCCGTTCTTAACTAGCATCAGAGAACACAC ATAAAATTAGACTCAATCTCAGAGAATTTCTACTAATTTAAGAAGCCATTGATGTAAGAAAAGTCTCTAG CTCATCTCCATGTATAGACCTATCTATTACTTTAGGGAATGTGAAAAGACTGTGTGTGGTCAAATCTTTT GTGAGATCATTTAGTATATACAGGTGAATTTCCCTTTGAATATAAAGAATACTAAAGCACTTTTTTTTTT CTTAAATTATAAAGCACATTCAATGGCAGGAAATCTCATCCAGACTTTATCAATATATATGACATGGAAA ATGTTGATACCTAAGTCATGCCTCAGTCTACAGAAAACCTCATAAAGTAGTGGCGCACAGCTAATGATTT AGCCAGGCACACCTTTGCATTCTGACCCATGTGAGTGATCTTGGCAAGACTCATTTGGACTTGATGTTTA ACAGCACAGCAAGACACAAAATCAAGGCGGAGGAAATCTCGGCATGGAGGTGCTCAGCTCTCATCCTAGC ACTCGGGGCAGTGTCAGATGCATCAGTGTGAGTTTGTGCTGACCTCTTCCAAATAGAGTGTTCTGGGCCA GCTAGGGATCACAGTTAAATCCTGTCATGAAAAAAGTGAGAAAATGTTAAATGGCAATAAAACCCATAAG AAAGAATATAAAGGACCTTTTAAATAGTACTTGAAACTCAACTGACATCAGAAACACAACAAAATACACT AAAGCGTAGGAGTGTGATACAGGAGTACAGGTGACACCTTACATCATCATATTACATCACCATGTTACAT CATCAGGATGATTGCAGTATTTAGTTGTGGACACACACAGTAGGAACCCCATCAGTCTATAGGTTATAAA AGCTGCACTCACACTTTAGCAAGCATGACATCAAGTATGAAAGTGAAAACACATTTGAATGTAAAAAATG AGCTTGAGTGTGGCACGGTCACACATGCTTGTGAAACACACACATTTCCTGTTCTTCTGGGTTTGAGAGT TCTTACACATCACCTGGAGTTTCAACATTCATGCCCATGAGTCCATTTTTGGAGAATATAAAGCAATTCT GAAGAAGTTGCAGCAAATTGATCATGACTTTTTGAGTGCTGTCTGTAAAATCACACCTTGTTTCATAAGA CAAGTTCTACATATGCAGGCCAGGCTGACTATGAATTTCTTGTATCATGGGCAGTTCTCATATTCACAGT CATACTGATGCAGGCTCTTGAGATCTTGGATTAGAGGAATGGTCTTCCGTGCAACTTTAGTATGTGACAT TTTCAGTGAAATTTCAACATTTTTATACACCAAAAATTTGGGGGAGAAAATTCAGTTTATGTAACTTGCA CGTTAATTTCCAGATGGAATTGTCTGCGATGGCATTGGAAATGAAGCAGCCAACTGTATTCTACCAAATA AACACAATGATGTTTCTAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3496918 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3496918 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14721 same embryo
	EMAGE:14716 same embryo
	EMAGE:14720 same embryo
	EMAGE:14719 same embryo
	EMAGE:14717 same embryo
	EurExpress:euxassay_005671 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source